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Preparation method and application of total nutrient microorganism biocontrol organic fertilizer

A microbial, full-nutrient technology, applied in the preparation of organic fertilizers, biological organic part treatment, organic fertilizers, etc., can solve the problems of poor biological fertilizers and other problems, and achieve the effects of long duration, high control efficiency and fast response.

Active Publication Date: 2014-10-22
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention aims at problems such as soil-borne diseases and poor effect of existing biological fertilizers, and provides a pollution-free, efficient and practical biological fertilizer preparation method and application

Method used

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  • Preparation method and application of total nutrient microorganism biocontrol organic fertilizer
  • Preparation method and application of total nutrient microorganism biocontrol organic fertilizer
  • Preparation method and application of total nutrient microorganism biocontrol organic fertilizer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] A kind of preparation method of whole nutrient microbial biocontrol organic fertilizer, comprises the steps:

[0068] (1) Inoculate Trichoderma pseudokiningii TRIPI and Rhizopus nigricans RN-9 on the PDA plate medium, and culture the plate at 28°C for 48 hours;

[0069] (2) Trichoderma pseudokiningii (Trichoderma pseudokiningii) TRIPI after step (1) plate culture and Rhizopus nigricans (Rhizopus nigricans) RN-9 are punched at the edge of the colony with an internal diameter of 5mm puncher, and inoculated with The hyphae block was picked by ring and transferred to PDB medium, and the PDB liquid culture was carried out at 28°C and 120rpm for 10 days to obtain a liquid culture solution;

[0070] (3) Inoculate the liquid culture solution prepared in step (2) in the bran glucose medium at 1:1, and ferment and cultivate it for 5 days at 25° C. and 100 rpm to obtain a fermentation solution;

[0071] The components per liter of the above-mentioned bran glucose medium are as fo...

Embodiment 2

[0078] A kind of preparation method of whole nutrient microbial biocontrol organic fertilizer, comprises the steps:

[0079] (1) Inoculate Trichoderma pseudokiningii TRIPI and Rhizopus nigricans RN-9 on the PDA plate medium, and culture the plate at 25° C. for 60 h;

[0080] (2) Trichoderma pseudokiningii (Trichoderma pseudokiningii) TRIPI after step (1) plate culture and Rhizopus nigricans (Rhizopus nigricans) RN-9 are punched at the edge of the colony with an internal diameter of 5mm puncher, and inoculated with Ring-picked mycelium pieces were transferred to PDB medium, and PDB liquid culture was carried out at 25°C and 150 rpm for 15 days to obtain a liquid culture solution;

[0081] (3) Inoculate the liquid culture solution prepared in step (2) in the bran glucose medium at a volume ratio of 1:2, and ferment and cultivate for 3 days at 30° C. and 150 rpm to obtain a fermentation solution;

[0082] The components per liter of the above-mentioned bran glucose medium are as...

Embodiment 3

[0089] A kind of preparation method of whole nutrient microbial biocontrol organic fertilizer, comprises the following steps:

[0090] (1) Inoculate Trichoderma pseudokiningii TRIPI and Rhizopus nigricans RN-9 on the PDA plate medium, and culture the plate at 30° C. for 36 hours;

[0091] (2) Trichoderma pseudokiningii (Trichoderma pseudokiningii) TRIPI after step (1) plate culture and Rhizopus nigricans (Rhizopus nigricans) RN-9 are punched at the edge of the colony with an inner diameter of 5mm puncher, and inoculated with The hyphae block was picked by ring and transferred to PDB medium, and the PDB liquid culture was carried out at 30°C and 100rpm for 8 days to obtain a liquid culture solution;

[0092] (3) Inoculate the liquid culture solution prepared in step (2) in the bran glucose medium at a volume ratio of 1:3, and ferment and cultivate it for 4 days at 28° C. and 120 rpm to obtain a fermentation solution;

[0093] The components per liter of the above-mentioned bra...

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Abstract

The invention relates to a preparation method and application of total nutrient microorganism biocontrol organic fertilizer. The preparation method of the total nutrient microorganism biocontrol organic fertilizer comprises the following steps: firstly, carrying out plate cultivation on trichoderma pseudokoningii TRIPI and rhizopus nigricans RN-9; secondly, carrying out PDB liquid culture on TRIPI and RN-9 hypha blocks; thirdly, carrying out fermentation culture on a liquid culture solution, so that fermentation liquor is obtained; fourthly, inoculating the fermentation liquor to farmyard manure raw materials, uniformly mixing, stockpiling and fermenting, so that a fermented material is obtained; fifthly, adding other ingredients into the fermented material, and uniformly mixing, so that the total nutrient microorganism biocontrol organic fertilizer is obtained. The prepared total nutrient microorganism biocontrol organic fertilizer has high inhibition ratio on pathogenic bacteria and high relative control efficiency on fusarium wilt of cucumber; compared with biocontrol organic fertilizer prepared by adopting single strain fermentation liquor, content of pathogenic bacteria in cucumber rhizosphere soil, cucumber root system and cucumber stalk is low, and cucumber blade protective enzyme is quick in response, high in activity and long in duration of time.

Description

technical field [0001] The invention relates to a preparation method and application of a full-nutrient microbial biocontrol organic fertilizer, which belongs to the technical field of compound microbial fertilizers. Background technique [0002] Soil refers to the loose material layer covering the land surface of the earth, which has the characteristics of fertility and can grow green plants. The main components are minerals, organic matter, microorganisms, water, etc. There are many types of microorganisms contained in the soil, mainly including bacteria, fungi, actinomycetes, algae and protozoa. The more fertile the soil, the more microbes there are. However, due to the increase in the world's population, the housing area and the urban area have increased, resulting in a sharp decrease in the land area. In order to pursue economic benefits, farmers began to overuse land, resulting in a series of agricultural problems, among which soil-borne diseases are particularly ser...

Claims

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Application Information

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IPC IPC(8): C05F17/00C05G3/04C05G3/80
CPCY02W30/40
Inventor 陈靠山樊恒达张鹏英于志丹周南郭艳玲王太林王静
Owner SHANDONG UNIV
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