Method for content determination and clustering analysis of caffeoylquinic acid components in periploca forrestii schltr

Abstract

The invention discloses a method for content determination and clustering analysis of caffeoylquinic acid components in periploca forrestii schltr. Quality of a periploca forrestii schltr medicinal material is comprehensively evaluated by measuring the content of the caffeoylquinic acid components in a periploca forrestii schltr ethyl acetate extract through an HPLC-UV method in combination with clustering analysis, principal component analysis and partial least square discriminant analysis. According to the method, the contents of six caffeoylquinic acid components are determined by combininga stoichiometric method; anti-rheumatoid arthritis efficacy quality of periploca forrestii schltr can be further evaluated, and a reference basis is provided for periploca forrestii schltr quality control; and the method has beneficial effects of good stability, good repeatability, high accuracy, a good periploca forrestii schltr chemical component separation effect, good chromatographic peak separation, a high response value and complete information.

Description

technical field [0001] The invention relates to a method for content determination and cluster analysis, in particular to a method for content determination and cluster analysis of caffeoylquinic acid components in S. Background technique [0002] Black bone vine is the dried root or whole plant of Periplocaforrestii Schltr., a plant of the genus Periploca in the family Asclepiadaceae. It is used by ethnic minorities in Guizhou Province. It is recorded in "Zhonghua Materia Medica" (Miao Medicine Volume) . Bitter in the mouth, pungent, has the effect of expelling wind and removing dampness, promoting blood circulation and eliminating carbuncle, and is used for rheumatic arthralgia, injury from falls, and irregular menstruation. Preliminary literature research shows that S. japonica has anti-rheumatoid arthritis (RA), cardiotonic, anti-tumor and immunosuppressive effects, and its main chemical components are mainly quinones, flavonoids, phenylpropanoids, etc. Steroids, terpe...

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Problems solved by technology

And the quality control method of Rhizoma Aegypti is mainly based on " the quality standard of Chinese medicinal materials of Guizhou Province, ethnic medicinal materials " (version 2003) at present, but in this standard the detection to Aegyptis is limited to proterties, microscopic and part physicochemical identification test, can not Very good control of the quality of the medicinal materials of the black bone vine

These functional components are used as the index components for the determination of the content of the effective part of the anti-RA of Aurantia chinensis, combined with the chemometric method, for the quality evaluation of the anti-RA efficacy of A.

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