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Anti-pathogen hyperimmune yolk antibody, AAV vector vaccine based preparation method and preparation

A carrier vaccine and egg yolk antibody technology, applied in the field of virus vaccines, can solve the problems that antibodies cannot be obtained by one-time immunization, protein immune stimulation, etc., and achieve the effects of extensive tissue infection ability, high safety and good immunogenicity.

Pending Publication Date: 2020-07-14
鲲羽生物科技(江门)有限公司
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0017] The technical problem to be solved by the present invention is that the current anti-new coronavirus hyperimmune egg yolk antibody cannot be obtained by one-time immunization, there is a safety risk in inactivating pathogens, and protein immunization cannot continuously stimulate the immune system.

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  • Anti-pathogen hyperimmune yolk antibody, AAV vector vaccine based preparation method and preparation
  • Anti-pathogen hyperimmune yolk antibody, AAV vector vaccine based preparation method and preparation
  • Anti-pathogen hyperimmune yolk antibody, AAV vector vaccine based preparation method and preparation

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Embodiment Construction

[0067] At present, studies have proved that the new coronavirus SARS-CoV-2, like SARS-CoV, mediates the infection of the virus on the host through the binding of Spike protein on the surface of the virus to the receptor angiotensin converting enzyme 2 (ACE2) on the surface of the host cell. Invasion of cells, but some studies have also proved that IgG targeting the full-length Spike protein of SARS can mediate the SARS virus to infect macrophages and aggravate the infection.

[0068] For this reason, the present invention provides a kind of anti-pathogen hyperimmune egg yolk antibody, method and preparation based on AAV vector vaccine preparation, to prepare the egg yolk antibody against SARS-CoV-2 virus Spike protein and be used for preventing new coronavirus infection. It is more advantageous to use partial fragments of the SARS-CoV-2 virus Spike protein, such as the S1 protein or the receptor-binding domain (RBD) gene as an antigen to prepare egg yolk antibodies. The presen...

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Abstract

The invention discloses an anti-pathogen hyperimmune yolk antibody, an AAV vector vaccine based preparation method and a preparation. The preparation method of the hyperimmune yolk antibody comprisesthe steps of selecting a first gene sequence containing an antigen protein fragment from a genomic sequence of a pathogen, and synthesizing a pathogen protective antigen gene through codon optimization; packaging a recombinant AAV vector vaccine carrying the antigen gene; and immunizing a healthy laying hen, and preparing the yolk antibody from a hen egg of the laying hen. According to the method,recombinant adeno-associated viruses with high safety, quick and simple packaging, low cost and stable expression are used as the vector vaccine for expressing protective antigen proteins of virusesor bacteria, the hen egg can be collected by immunizing the healthy laying hen once, the antivirus or antibacterial yolk antibody as an active component in yolk is extracted, and the antibody is natural immune globulin and can kill the viruses or bacteria and prevent viral or bacterial infection.

Description

technical field [0001] The invention relates to the technical field of virus vaccines, in particular to an anti-pathogen hyperimmune egg yolk antibody and a preparation method and preparation based on an AAV vector vaccine. Background technique [0002] Viruses and bacteria have coexisted with humans on the earth for thousands of years, and humans have developed immunity to many viruses and bacteria. However, there is still no resistance to some viruses, and the viruses are also mutating and evolving over time, such as influenza viruses that are less harmful to humans, and plague that is fatal to humans. Therefore, in order to resist the attack of virus, multiple virus vaccines have occurred. Virus vaccines are generally injections. After one or more injections, virus antibodies are produced in the body, thereby preventing infection by such viruses. [0003] On February 11, 2020, the International Committee on Taxonomy of Viruses officially named the new coronavirus in Eng...

Claims

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Application Information

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IPC IPC(8): C12N15/66C12N15/85C07K16/10C07K16/02A61K39/42A61K9/12A61K31/04A61K31/12A61K31/14
CPCC12N15/66C12N15/85C07K16/10C07K16/02A61K9/12A61K47/44A61K31/04A61K31/12A61K31/14A61K2039/505
Inventor 闫科技
Owner 鲲羽生物科技(江门)有限公司
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