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Single-domain antibodies and variants thereof against pd-l1

A single-domain antibody, PD-L1 technology, applied in the direction of antibodies, antibody medical components, anti-animal/human immunoglobulin, etc., can solve problems such as unmet medical needs and uncommercialization

Pending Publication Date: 2020-07-17
NANJING LEGEND BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is a clear unmet medical need as treatments targeting targets in this pathway are not yet commercially available

Method used

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  • Single-domain antibodies and variants thereof against pd-l1
  • Single-domain antibodies and variants thereof against pd-l1
  • Single-domain antibodies and variants thereof against pd-l1

Examples

Experimental program
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Effect test

Embodiment approach 1

[0347] Embodiment 1 is an isolated anti-PD-L1 construct comprising a single domain antibody (sdAb) portion that specifically recognizes PD-L1, wherein the sdAb portion comprises CDR1, CDR2 and CDR3, and said CDR1 comprises SEQ The amino acid sequence of any one of ID NO: 51-100 or a variant thereof comprising up to about 3 amino acid substitutions, said CDR2 comprising the amino acid sequence of any one of SEQ ID NO: 151-200 or a variant thereof comprising up to about 3 A variant of amino acid substitutions, the CDR3 comprising the amino acid sequence of any one of SEQ ID NO: 251-300 or a variant comprising up to about 3 amino acid substitutions.

Embodiment approach 2

[0348] Embodiment 2 is the isolated anti-PD-L1 construct of embodiment 1, wherein the sdAb portion comprises CDR1 comprising the amino acid sequence of any one of SEQ ID NO: 51-100; comprising any one of SEQ ID NO: 151-200 The amino acid sequence of CDR2 and CDR3 comprising the amino acid sequence of any one of SEQ ID NO: 251-300; or a variant thereof, the variant comprising up to about 3 amino acid substitutions in the plurality of CDR regions.

Embodiment approach 3

[0349] Embodiment 3 is the isolated anti-PD-L1 construct of any of embodiments 1-2, wherein the sdAb portion comprises any of the following:

[0350] (1) CDR1 comprising the amino acid sequence of SEQ ID NO: 51; CDR2 comprising the amino acid sequence of SEQ ID NO: 151; and CDR3 comprising the amino acid sequence of SEQ ID NO: 251; or a variant thereof, the variant being The plurality of CDR regions comprise up to about 3 amino acid substitutions;

[0351] (2) CDR1 comprising the amino acid sequence of SEQ ID NO: 52; CDR2 comprising the amino acid sequence of SEQ ID NO: 152; and CDR3 comprising the amino acid sequence of SEQ ID NO: 252; or a variant thereof, the variant being The plurality of CDR regions includes up to about 3 amino acid substitutions;

[0352] (3) CDR1 comprising the amino acid sequence of SEQ ID NO: 53; CDR2 comprising the amino acid sequence of SEQ ID NO: 153; and CDR3 comprising the amino acid sequence of SEQ ID NO: 253; or a variant thereof, the variant being ...

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PUM

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Abstract

Provided are constructs comprising a single-domain antibody (sdAb) moiety that specifically recognizes PD-L1. Also provided are methods of making and using these constructs.

Description

[0001] Submit sequence list as ASCII text file [0002] The submission of the following ASCII text file is incorporated herein by reference in its entirety: Sequence Listing in Computer Readable Format (CRF) (File name: 688096.97 Sequence Listing, date of indication: November 1, 2017, size: 271kb). [0003] Invention field [0004] The present invention relates to a construct containing a single domain antibody (sdAb) portion that specifically recognizes PD-L1, and methods of preparation and use thereof. [0005] Background of the invention [0006] The immunosuppressive receptor expressed mainly on activated T cells and B cells, programmed cell death receptor 1, also known as programmed death receptor 1 (PD-1), is related to CD28 and cytotoxic T lymphocytes A member of the related immunoglobulin superfamily related to protein-4 (CTLA-4). PD-1 and similar family members are type I transmembrane glycoproteins, which contain an extracellular Ig variable (V-type) domain that binds to its ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/28A61P35/00A61P31/00C12N15/13C12N15/85C12N5/10A61K39/395
CPCC07K16/2827A61K2039/505C07K2317/24C07K2317/569C07K2317/73C07K2317/76C07K2317/92A61P31/00A61P35/00C07K2317/22C07K2317/52A61K39/3955A61K45/06C07K2317/21C07K2317/31
Inventor 张亚峰武术杨帅周传初
Owner NANJING LEGEND BIOTECH CO LTD