A kind of piRNA and the test kit for detecting and/or predicting male reproductive dysfunction

A detection reagent and reagent technology, applied in the field of RNA detection, can solve problems such as lack of testicular reserve function

Active Publication Date: 2021-12-07
深圳中山泌尿外科医院
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the prior art, there is a lack of a way to use piRNA to quantitatively detect RNA to reflect the testicular reserve function of the reproductive system

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of piRNA and the test kit for detecting and/or predicting male reproductive dysfunction
  • A kind of piRNA and the test kit for detecting and/or predicting male reproductive dysfunction
  • A kind of piRNA and the test kit for detecting and/or predicting male reproductive dysfunction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] A method for quantitative detection of RNA in seminal plasma, comprising the steps of:

[0041] S1. To collect seminal plasma samples, the steps are as follows:

[0042] Male abstinence for 2 to 7 days, use the masturbation method to collect all the semen in a clean wide-mouth non-toxic plastic container. Specimens were incubated at 37°C until fully liquefied. Within 2 hours of sampling, the samples were centrifuged at 1500g for 5 minutes at room temperature to separate the sperm and seminal plasma. Pipette the supernatant into a new Eppendorf tube, centrifuge at 12,000g at 4°C for 5 minutes to remove cells and debris, pipette the supernatant seminal plasma into a new Eppendorf tube and freeze at -80°C for later use.

[0043] S2, the selection of relative quantitative reference, the steps are as follows:

[0044] (1) Chemical synthesis: the sequence of the synthetic Caenorhabditis elegans cel-miR-39-3p is

[0045] 5'-UCACCGGGUGUAAAAUCAGCUUG-3'. The synthetic produc...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to the field of RNA detection, in particular to a piRNA and a kit for detecting and / or predicting male reproductive dysfunction. The sequence of the reverse transcription primer of the piRNA is 5'-GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACNNNNNNNN-3'. A kit for detecting and / or predicting male reproductive dysfunction, including Caenorhabditis elegans cel-miR-39-3p and primers for detecting piR-32678 and cel-miR-39-3p by SYBR Green Real-time PCR method. The reverse transcription primer of piRNA in the present invention can quantitatively detect RNA, thereby reflecting the health status of the testicular reserve function of the reproductive system, and further helping to judge the etiology of male azoospermia and oligospermia.

Description

technical field [0001] The invention belongs to the field of RNA detection, and in particular relates to a piRNA and a kit for detecting and / or predicting male reproductive dysfunction. Background technique [0002] Infertility has become a global medical and sociological problem that affects human development and social harmony, and brings a heavy burden to family happiness and social harmony. According to the 2012 "China Infertility Status Survey Report", the infertility rate among the population of childbearing age in my country has reached as high as 12.5%, especially in economically developed areas, the incidence of infertility has even exceeded 15%. Male infertility accounts for about 50% of infertility, of which spermatogenesis disorder is the main cause. Impaired spermatogenesis refers to poor spermatogenesis in the seminiferous tubules, clinically manifested as oligozoospermia, weak, teratozoospermia, or non-obstructive azoospermia (NOA). More than half of the sper...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11C12Q1/6883
CPCC12Q1/6883C12Q2600/158C12Q2600/166C12Q2600/178
Inventor 吴彤华李龙飞宋明哲叶丽君陈春媚曾勇
Owner 深圳中山泌尿外科医院
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap