Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A screening method, molecular marker, primer and application of a molecular marker linked to a soybean cyst nematode resistance locus

A technology of soybean cyst nematodes and molecular markers, applied in the field of genetic breeding, can solve the problems of heavy workload, less QTL analysis, and difficulty in popularization and application, and achieve remarkable results and reduce the number of cysts

Active Publication Date: 2022-06-03
NORTHEAST AGRICULTURAL UNIVERSITY
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This locus is difficult to be popularized and applied due to technical means, cost and difficulty of operation, and requires high operator experience. In the case of multiple genetic backgrounds of the same physiological race and the same genetic background of multiple physiological races, the workload is relatively large, and Fewer QTL analyzes were obtained

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A screening method, molecular marker, primer and application of a molecular marker linked to a soybean cyst nematode resistance locus
  • A screening method, molecular marker, primer and application of a molecular marker linked to a soybean cyst nematode resistance locus
  • A screening method, molecular marker, primer and application of a molecular marker linked to a soybean cyst nematode resistance locus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055]

[0056]

[0059]

Embodiment 2

[0062] Example 2. Screening methods for molecular markers.

[0080] SSR_16_02-F and SSR_16_02-R primer pair obtained molecular marker is SSR_16_02;

[0081] SSR_16_03-F and SSR_16_03-R primer pair obtained molecular marker is SSR_16_03;

[0082] SSR_16_04-F and SSR_16_04-R primer pair obtained molecular marker is SSR_16_04;

[0084] SSR_16_06-F and SSR_16_06-R primer pair obtained molecular marker is SSR_16_06;

[0086] The molecular markers obtained by the SSR_16_08-F and SSR_16_08-R primer pairs are the SSR_16_08 molecular markers.

[0088]

[0090] rhg1 and Rhg4 site SNP analysis:

[0096] There is a mutation of cytosine and guanine (C / G) at 108bp on the Rhg4 PCR amplification product (Fig. 8), which is resistant to disease

[0098] a. QTL analysis of cyst nematode race 3 under multiple genetic backgrounds.

[0101]

[0104]

[0108]

[0109] Samples from this population containing major loci rhg1 and Rhg4 were removed and mapped (Table 7).

[0111]

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

A screening method, molecular marker, primer and application of a molecular marker linked to a soybean cyst nematode resistance locus belong to the technical field of genetic breeding. Aiming at the problem of how to obtain the main effective disease-resistant locus or disease-resistant gene for resisting soybean cyst nematodes on the same genetic background of the same physiological race and the same genetic background of multiple physiological races, the present invention provides a soybean cyst nematode-resistant The molecular marker linked to the resistance locus is located on chromosome 16 of soybean (J linkage group), and the molecular marker is one of SSR_16_01, SSR_16_02, SSR_16_03, SSR_16_06, SSR_16_07 and SSR_16_08, and the primers for each molecular marker As shown in SEQ ID NO: 1-12. The invention can be used for screening the cyst nematode resistance gene or for soybean cyst nematode resistance breeding.

Description

A screening method for molecular markers linked to soybean cyst nematode resistance loci Methods, molecular markers, primers and applications technical field The invention belongs to the technical field of genetic breeding, be specifically related to a kind of linkage with soybean resistance to cyst nematode disease resistance site Screening methods, molecular markers, primers and applications of molecular markers. Background technique There are many resistance sites of multiple physiological races in SCN, and the study found that Yue adopts a F 2 generational groups, When the resistance loci of each physiological race were tested, RFLP markers and SSR markers were used respectively, and the results showed that the loci located in An interval on Chr.18 (LG G) aggregates loci with resistance to multiple races, however, SCN 1, 2, 5 The loci with disease resistance of physiological races clustered in a certain interval on Chr.11 (LG B1). At present, among many di...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 韩英鹏赵雪李文滨吴德鹏王伟
Owner NORTHEAST AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com