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Screening method of molecular marker linked with soybean cyst nematode resistance locus, molecular marker, primers and application

A technology of molecular markers and cyst nematodes, applied in the field of genetic breeding, can solve the problems of high operator experience requirements, less QTL analysis, and difficulty in popularization and application

Active Publication Date: 2020-07-31
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This locus is difficult to be popularized and applied due to technical means, cost and difficulty of operation, and requires high operator experience. In the case of multiple genetic backgrounds of the same physiological race and the same genetic background of multiple physiological races, the workload is relatively large, and Fewer QTL analyzes were obtained

Method used

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  • Screening method of molecular marker linked with soybean cyst nematode resistance locus, molecular marker, primers and application
  • Screening method of molecular marker linked with soybean cyst nematode resistance locus, molecular marker, primers and application
  • Screening method of molecular marker linked with soybean cyst nematode resistance locus, molecular marker, primers and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Example 1. Soil propagation and resistance identification of soybean cyst nematode disease.

[0053] In this study, the recombinant inbred line populations constructed from the three combinations of Heinong 37×Dongnong L-10, Suinong 10×Dongnong L-10, and Suinong 14×Dongnong L-10 were evaluated against soybean cyst nematodes. For the identification of the resistance of No. 3 physiological race, the acquisition process of the recombinant inbred line population is as follows: through the self-propagation of the F1 generation single grain derived from the above-mentioned parental combination hybridization to obtain the F2 generation single grain, continuous selfing from the F2 generation, combining The offspring were reserved to the F6 generation by the single seed transmission method, and the line propagation was carried out from the F6 generation to the F11 generation. The map construction and disease resistance identification of the F11 generation were carried out, and th...

Embodiment 2

[0062] Example 2. Screening method for molecular markers.

[0063] Molecular marker analysis of parents and offspring:

[0064] (1) The soybean variety Dongnong L-10 was used as the male parent, and the soybean varieties Heinong 37, Suinong 10, and Suinong 14 were used as the female parents, and the recombinant inbred line F was obtained after crossing 5:11 Generation: Heinong 37×Dongnong L-10, Suinong 10×Dongnong L-10, Suinong 14×Dongnong L-10; See Example 1 for the breeding method of the recombinant inbred lines.

[0065] (2) Extract the parental genome DNA of Dongnong L-10, Heinong 37, Suinong 10, and Suinong 14 respectively, and use SSR primers for PCR amplification to screen polymorphic SSR primers; the method is as follows:

[0066]According to the previous report on soybean cyst nematode resistance loci in this study, the SSR marker loci in the target QTL (qSCN16) segment (31.46Mb-35.79Mb) on Chr.16 (LGJ) linkage group of soybean chromosome 16 were selected Spot, and ...

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Abstract

The invention discloses a screening method of a molecular marker linked with a soybean cyst nematode resistance locus, the molecular marker, primers and application, and belongs to the technical fieldof genetic breeding. Aiming at a problem of how to obtain a major disease-resistant locus or a disease-resistant gene for resisting soybean cyst nematode under the condition of same physiological race and multi-genetic backgrounds and under the condition of multi physiological races and same genetic background, the invention provides the molecular marker linked with the soybean cyst nematode resistance locus. The molecular marker is located in the 16th chromosome (J linkage group) of soybeans. The molecular marker is one of SSR_16_01, SSR_16_02, SSR_16_03, SSR_16_06, SSR_16_07 and SSR_16_08,and the primers of the molecular marker are shown as SEQ ID NO: 1 to SEQ ID NO: 12. The method can be used for screening an anti-cyst nematode gene or breeding anti-cyst nematode soybeans.

Description

technical field [0001] The invention belongs to the technical field of genetic breeding, and in particular relates to a screening method, molecular marker, primer and application of a molecular marker linked to a soybean cyst nematode resistance locus. Background technique [0002] There are many race-resistant loci in SCN, the study found that Yue adopts an F 2 When testing the loci with resistance to each physiological race, RFLP markers and SSR markers were used respectively. The results showed that a section located on Chr. Race-resistant loci, however, loci with resistance to SCN 1, 2, and 5 physiological races gathered in a certain interval on Chr.11 (LG B1). Among the many disease resistance loci, the rgh1 locus on LG G and the Rhg4 locus on LG A2 are relatively recognized resistance loci. This is of great significance for the study of soybean production efficiency, the selection of high-quality soybean resources, and the screening of candidate genes for disease res...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 韩英鹏赵雪李文滨吴德鹏王伟
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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