Method for detecting tetracycline in milk based on surface enhanced Raman technology

A technical detection, tetracycline technology, applied in the field of Raman spectroscopy, can solve the problems of lack of advantages and low detection limit, and achieve the effect of improving detection ability, strengthening grasping, and enhancing Raman signal

Active Publication Date: 2020-08-18
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, in the field of antibiotic detection, some scholars have proposed to use SERS technology to detect tetracycline in aqueous solution, but there are ve...

Method used

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  • Method for detecting tetracycline in milk based on surface enhanced Raman technology
  • Method for detecting tetracycline in milk based on surface enhanced Raman technology
  • Method for detecting tetracycline in milk based on surface enhanced Raman technology

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Experimental program
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Embodiment 1

[0042] 1. Preparation of SERS substrate based on PDMS cavity

[0043] (1) Preparation of silver nitrate solution

[0044] a. preparation concentration is the silver nitrate aqueous solution of 50 μ g / mL;

[0045] (2) Preparation of PDMS glue

[0046] a. Take part A and part B of Dow Corning Sylgard-184PDMS glue with a mass ratio of 10:1, and stir rapidly and vigorously to exhaust the air bubbles;

[0047] b. Place in a 23°C thermostat for 72 hours.

[0048] (3) Preparation of SERS substrate based on PDMS cavity

[0049] a. Take the prepared 100 μL silver nitrate solution and drop it into the cured PDMS glue with a pipette gun;

[0050] b. The PDMS substrate dropped into the silver nitrate solution was placed in a thermostat at 23° C. for 72 hours to cure.

[0051] 2. Detection of tetracycline based on PDMS lumen SERS substrate

[0052] (1) Dilute the tetracycline mother liquor, configure the complete concentration gradient standard solution, mix the concentration of 0.25mo...

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Abstract

The invention discloses a method for detecting tetracycline in milk based on a surface enhanced Raman technology, and belongs to the technical field of Raman spectra. According to the invention, the nano-silver substrate with a PDMS cavity structure is used as a Raman enhanced substrate, and 4-amino-1-butanol and the SERS substrate are combined for use, so that the SERS enhancement is superior tothat of single use of a PDMS cavity or nano-silver particles. By adding 4-amino-1-butanol, SERS enhancement is better than that of single use of a PDMS cavity or nano-silver particles, and the tetracycline detection limit can reach 0.2 [mu] g/L. The PDMS plasma cavity can be used as an SERS substrate to detect tetracycline in milk, a milk sample does not need to be pretreated, the detection time is within ten minutes, the detection limit is as low as 0.2 [mu] g/L, and the method has important significance in monitoring abuse and residue of tetracycline antibiotics.

Description

technical field [0001] The invention relates to a method for detecting tetracycline in milk based on surface-enhanced Raman technology, and belongs to the technical field of Raman spectroscopy. Background technique [0002] Tetracycline is a commonly used veterinary antibiotic with antimicrobial activity in the treatment and prevention of disease. However, the misuse of tetracyclines has led to the widespread dissemination of resistance genes in other animals and humans. In practical settings, low concentrations of tetracycline residues can still cause health damage. In the end, tetracycline will be enriched in the human body through foods such as milk and eggs, and eventually endanger human health. [0003] At present, the main methods for detecting tetracycline in milk are: chromatography, high performance liquid chromatography, and enzyme-linked immunosorbent assay. These methods have high precision and reliability, but most of the detection steps are complex and time-...

Claims

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Application Information

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IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 陈国庆杨子晨吴亚敏马超群李磊朱纯辜姣高辉
Owner JIANGNAN UNIV
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