Preparation method of injectable child epiphyseal plate regenerated hydrogel

An injection-type, water-regenerating technology, applied in the field of biomedical materials, can solve the problems of uncontrollable longitudinal growth of long bones, low cartilage-forming efficiency of scaffolds, and no layered structure, so as to be beneficial to popularization and use, and to promote the regeneration and repair of epiphyseal plate cartilage. , the effect of great market application potential

Active Publication Date: 2020-09-04
NANJING CHILDRENS HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although a lot of research has been done before, using different seed cells, screening different types of bioactive materials and regulation methods, but still facing the problems of low scaffolding cartilage formation efficiency and poor fusion of regenerated cartilage and surrounding normal tissues
More importantly, the regenerated cartilage does not have a layered structure similar to the natural epiphyseal plate, and cannot control the longitudinal growth of long bones

Method used

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  • Preparation method of injectable child epiphyseal plate regenerated hydrogel
  • Preparation method of injectable child epiphyseal plate regenerated hydrogel
  • Preparation method of injectable child epiphyseal plate regenerated hydrogel

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Embodiment 1

[0030] 1) Preparation of a sodium alginate double-layer drug-loaded microsphere

[0031] In this embodiment, the preparation of sodium alginate double-layer drug-loaded microspheres is mainly carried out by the flow focusing method and the microfluidic control method, and the main steps are as follows:

[0032] 1. Such as figure 2 As shown, the microfluidic chip is placed on the stage of the optical microscope, and six hoses are respectively connected to extend the six channel ports;

[0033] 2. Add 1% sodium alginate solution with IGF-1 to the single-channel syringe pump through a syringe, exhaust the air, and connect it to the No. 2 channel port through a hose;

[0034] 3. Draw 1% sodium alginate solution into two syringes, place them in a dual-channel syringe pump, and connect them to the two No. 1 channel ports through a hose;

[0035] 4. Use two syringes to draw 1% CaCl 2 The paraffin oil is placed in a dual-channel injection pump, which is connected to two No. 3 channel ports;

[...

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Abstract

The invention discloses a preparation method of injectable child epiphyseal plate regenerated hydrogel. The preparation method comprises the main steps as follows: 1, preparing double-layer drug-loading sodium alginate (SA) microspheres with an anti-bone bridge formation factor as an outer layer and a cartilage differentiation promoting factor as an inner layer; and 2, mixing the SA microspheres and bone marrow mesenchymal stem cells (BMSCs) in hydrogel to prepare biological ink. The hydrogel is injected in an injured part of epiphyseal plate cartilage, bone bridge formation can be inhibited and regeneration and repair of the epiphyseal plate cartilage can be promoted later through local, timed and quantitative release of Enbrel + Bevacizumab and IGF-1, and a new thinking and method are produced for solving the problem of epiphyseal plate injury repair.

Description

Technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a preparation method of an injectable regenerated hydrogel for children's epiphyseal plates. Background technique [0002] The epiphyseal plate, also known as the growth plate, is the area of ​​cartilage that controls the longitudinal growth of the immature long bones of children. Fractures, infections, tumors, or iatrogenic injuries can all lead to epiphyseal plate damage. About 30% of children suffer from epiphyseal plate damage and bone growth imbalance after fracture. Once the epiphyseal plate is damaged, the cartilage tissue of the epiphyseal plate will gradually be replaced by bone tissue, forming a "bone bridge", which will cause growth retardation, angulation or rotation deformity, and even further affect joint function, which will have a huge impact on children's physical and mental health. The current clinical treatment methods are mainly bone bridge resection ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38A61L27/20A61L27/50A61L27/52A61L27/54
CPCA61L27/3834A61L27/3852A61L27/20A61L27/50A61L27/52A61L27/54A61L2400/06A61L2430/06A61L2300/414A61L2300/624C08L5/04
Inventor 郑朋飞王金武柳毅浩强磊王彩萍
Owner NANJING CHILDRENS HOSPITAL
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