Detection of modified live swine influenza virus vaccines
A technology of swine influenza virus and influenza virus, applied in the field of detection of modified live swine influenza virus vaccine, can solve the problems of undisclosed and undisclosed specific oligonucleotide probes
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[0535] The following examples are intended to illustrate the invention only. These examples should not limit the scope of the claims in any way.
[0536] Materials and methods
[0537] 1. Preparation of Primer / Probe Mixture
[0538] Table 1: Primer / Probe Sequences:
[0539]
[0540] Table 2: Primer / Probe Concentrations
[0541] Primer / Probe Mix Final concentration: NSfor gataataggctctctttgtg (SEQ ID NO: 1) 0.5μM NSrev aggtaatggtgaaatttctc (SEQ ID NO:2) 0.4μM WTfluprobe gtgtgatctttaaccgattatagagactttg (SEQ ID NO: 11) 0.25μM MLVfluprobe1 atggaaaagtagatcttgattaattaagagg (SEQ ID NO: 7) 0.25μM MLVfluprobe2 agtagatcttgattaattaagagggagc (SEQ ID NO: 5) 0.25μM
[0542] Primers and probes were purchased from Biosearch Technologies.
[0543] NS (nonstructural protein); for (forward); rev (reverse); WT (wild type); MLV (modified live virus)
[0544] 2. Preparation of Master Mix
[0545] Table 3: Preparation of master ...
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