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An eye drop suitable for corneal limbal stem cell deficiency and its preparation

A technology of corneal limbal stem cells and eye drops, which is applied in the field of medicine, can solve the problems of lack of corneal limbal stem cells, etc., and achieve the effect of preventing corneal epithelial conjunctivalization, low cost, and reducing economic burden

Active Publication Date: 2022-04-19
QINGDAO HAIER BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there are few reports on the use of exosomes extracted from stem cell culture medium for repairing corneal damage and relieving visual fatigue. Eye drops for neovascularization and conjunctivalization of the corneal epithelium, no related reports

Method used

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  • An eye drop suitable for corneal limbal stem cell deficiency and its preparation
  • An eye drop suitable for corneal limbal stem cell deficiency and its preparation
  • An eye drop suitable for corneal limbal stem cell deficiency and its preparation

Examples

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Comparison scheme
Effect test

Embodiment 1

[0058] An eye drop suitable for corneal limbal stem cell deficiency, comprising the following components:

[0059] Human adipose stem cell exosomes 1mg / L, excipients: sodium hyaluronate 0.5g / L, vitamin B6 1g / L, benzalkonium chloride 0.05g / L, the balance is normal saline for injection, pH 7.0.

[0060] The preparation method of above-mentioned eye drop, comprises the steps:

[0061] (1) Preparation of exosomes from human adipose-derived stem cells: select the third-generation human adipose-derived stem cells, use stem cell culture medium, and prepare exosomes at a volume concentration of 5% CO 2 Cultured in a constant temperature cell incubator at 37°C. After the cells were cultured to 80% confluence, they were replaced with MEM stem cell basal medium and cultured for 48 hours at a volume concentration of 5% CO 2 Cultured in a 37°C constant temperature cell incubator, collected the culture supernatant, and extracted exosomes by ultra-speed gradient centrifugation. Supernatant...

Embodiment 2

[0065] An eye drop suitable for corneal limbal stem cell deficiency, comprising the following components:

[0066] Human adipose stem cell exosomes 10mg / L, excipients: sodium hyaluronate 1g / L, vitamin B6 2g / L, benzalkonium chloride 0.1g / L, the balance is normal saline for injection, pH 7.0.

[0067] The preparation method of above-mentioned eye drop, comprises the steps:

[0068] (1) Preparation of exosomes from human adipose stem cells: select 3 generations of human adipose stem cells, use stem cell culture medium, and prepare exosomes at a volume concentration of 5% CO 2 Cultured in a constant temperature cell incubator at 37°C. After the cells were cultured to 80% confluence, they were replaced with MEM stem cell basal medium and cultured for 48 hours at a volume concentration of 5% CO 2 Cultured in a 37°C constant temperature cell incubator, collected the culture supernatant, and extracted exosomes by ultra-speed gradient centrifugation. Supernatant: 100000×g, 1h, remove...

Embodiment 3

[0072] An eye drop suitable for corneal limbal stem cell deficiency, comprising the following components:

[0073] Human adipose stem cell exosomes 20mg / L, excipients: sodium hyaluronate 2g / L, vitamin B6 3g / L, benzalkonium chloride 0.3g / L, the balance is normal saline for injection

[0074] The preparation method of above-mentioned eye drop, comprises the steps:

[0075] (1) Preparation of exosomes from human adipose stem cells: Select 4 generations of human adipose stem cells, use stem cell culture medium, and prepare exosomes at a volume concentration of 5% CO 2 Cultured in a constant temperature cell incubator at 37°C. After the cells were cultured to 80% confluence, they were replaced with MEM stem cell basal medium and cultured for 48 hours at a volume concentration of 5% CO 2 Cultured in a 37°C constant temperature cell incubator, collected the culture supernatant, and extracted exosomes by ultra-speed gradient centrifugation. Supernatant: 100000×g, 1h, remove the supe...

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Abstract

The invention relates to an eye drop suitable for limbal stem cell deficiency and a preparation method thereof, the eye drop comprising the following components: human adipose stem cell exosomes 1-40mg / L, auxiliary materials: sodium hyaluronate 0.5-2g / L, vitamin B6 0.5-3g / L, benzalkonium chloride 0.05-0.3g / L, the balance is medical saline, pH is 6.5-7.5; the eye drops involved in the present invention are used for the treatment of limbal stem cell deficiency The effect is remarkable.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to an eye drop suitable for limbal stem cell deficiency and a preparation method thereof. Background technique [0002] Limbal stem cells are a group of stem cells located at the Vogt barrier at the base of the limbus. Limbal stem cells proliferate and differentiate continuously to maintain the stability and renewal of the corneal epithelium. Ocular surface chemical damage or thermal burns, dry eye, long-term wearing of contact lenses (contact lenses) and other factors can cause decompensation of the number and function of limbal stem cells, resulting in the lack of limbal stem cells. Limbal stem cell deficiency makes the corneal epithelium lose its ability to regenerate and repair, leading to corneal epithelial conjunctivization, neovascularization, corneal stroma scarring, and even corneal autolysis and ulceration. Limbal stem cell deficiency is a common blinding eye...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0775A61K31/728A61K31/4415
CPCA61K35/28A61K31/728A61K31/4415A61K47/36A61K47/22A61K47/186A61K9/08A61K9/0048A61P27/02C12N5/0667C12N2509/00A61K2300/00A61K47/24C12N2500/38
Inventor 狄国虎曹启龙郭晶李慧胡艳华任巧盟
Owner QINGDAO HAIER BIOTECH CO LTD
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