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Probe library and kit for detecting lung cancer related 41 genes and application thereof

A kit and technology for lung cancer, applied in the field of biomedicine, can solve the problems of not being able to cover the spread of genes, and achieve the effects of good sequencing depth uniformity, maximum use, and reduced clinical testing costs

Pending Publication Date: 2020-10-09
CHONGQING UNIV CANCER HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the multiplex PCR used in this patent is only limited to some mutation sites on some exons of the EGFR gene, which obviously cannot cover enough gene distribution related to lung cancer treatment, so this patent has relatively large limitations in its use

Method used

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  • Probe library and kit for detecting lung cancer related 41 genes and application thereof
  • Probe library and kit for detecting lung cancer related 41 genes and application thereof
  • Probe library and kit for detecting lung cancer related 41 genes and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0201] 1. Build a sample library

[0202] 1. Extraction of DNA

[0203] Sample DNA was extracted using the HiPure FFPE DNA Kit (Magen) kit.

[0204] 2. DNA Fragmentation

[0205] According to the instruction manual of the DNA breaker (Bandelin), the DNA sample was fragmented so that the fragment length was 200-500 bases.

[0206] DNA was purified using the Beckman Coulter Ampure Beads kit (Cat. No. A63881).

[0207] 3. DNA sample bank quality inspection

[0208] Use Bio-Fragment Analyzer to conduct qualitative and quantitative analysis of DNA to confirm that the peak length of DNA fragments is reasonable.

[0209] 4. DNA end repair

[0210] Using T4 polymerase and Klenow E. coli polymerase fragments, blunt the 5' protruding sticky ends and 3' protruding sticky ends of DNA to generate blunt ends. At the same time, T4 polynucleotide kinase is used to phosphorylate the 5' end of DNA for subsequent blunt end ligation.

[0211]

[0212]

[0213] The reaction was carrie...

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Abstract

The invention provides a probe library and a kit for detecting lung cancer related 41 genes and an application of the probe library and the kit. Wherein the probe library comprises a lung cancer related 41 gene capture probe; the number of the lung cancer related 41 gene capture probes is 1464, and the nucleotide sequences of the lung cancer related 41 gene capture probes are shown as SEQ ID NO.1-1464. When the probe library is applied to the next-generation sequencing technology, the lung cancer related 41 genes can be detected at the same time in a high-throughput mode, and the lung cancer related 41 gene capture probes have the advantages of being high in coverage rate, high in accuracy and the like. The 41 genes related to lung cancer treatment sensitivity, resistance and toxic and side effects are incorporated into detection genes of the probe library; the detection result can directly evaluate the lung cancer treatment effect and toxic reaction in advance, can help to screen outproper drugs and treatment schemes, is helpful for doctors to make treatment decisions and reasonably take drugs for patients, and can also provide basic mutation spectrum data and detection strategies for future drug development and improvement of treatment accuracy.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a probe library, a kit and an application thereof for detecting lung cancer-related 41 genes. Background technique [0002] Lung cancer is the tumor with the highest incidence rate in my country. The treatment methods for lung cancer include surgery, chemotherapy, radiotherapy, and targeted therapy. How to choose the most suitable treatment method for each patient is the key to determine the treatment effect of lung cancer. With extensive research on molecular mechanisms such as drug resistance and drug resistance, people have a broad and in-depth understanding of the occurrence and development of lung cancer at the genetic level, and more and more molecularly targeted drugs for various specific gene mutation groups have entered or are entering clinical practice. Genetic testing plays an increasingly important role in lung cancer treatment decisions. At present, there are m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12Q1/6869C12N15/11C40B40/06
CPCC12Q1/6886C12Q1/6869C40B40/06C12Q2600/156C12Q2600/136C12Q2535/122
Inventor 施小龙吴永忠唐超王颖尹哲綦俊
Owner CHONGQING UNIV CANCER HOSPITAL
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