A method for establishing a non-obese non-alcoholic fatty liver disease model in rats

A technique for fatty liver disease and establishment of a method, which is applied in the field of establishing a non-obese non-alcoholic fatty liver disease model in rats, can solve problems such as incomplete elucidation of the mechanism and incomplete research on non-obese non-alcoholic fatty liver disease, etc. Simple modeling method

Active Publication Date: 2022-02-25
BEIHANG UNIV +2
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Problems solved by technology

Research on non-obese nonalcoholic fatty liver disease is incomplete and its mechanisms have not been fully elucidated

Method used

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  • A method for establishing a non-obese non-alcoholic fatty liver disease model in rats
  • A method for establishing a non-obese non-alcoholic fatty liver disease model in rats
  • A method for establishing a non-obese non-alcoholic fatty liver disease model in rats

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specific Embodiment approach

[0049] Experimental animals were provided by Beijing Huafukang Biotechnology Co., Ltd.;

[0050] Soybean is Zhonghuang 13 non-transgenic soybean, purchased from Chinese Academy of Agricultural Sciences;

[0051] The inflammatory factor Elisa kit was purchased from Jitai Yikesai Company;

[0052] The commercial standard rat feed was purchased from Beijing Huafukang Biotechnology Co., Ltd., the name and model were rat maintenance feed 1022, and its ingredients were as follows:

[0053] protein source soybean meal, fish meal fat source vegetable oil fiber source bran carbohydrate source corn, flour vitamins VA, VD, VE, VB1, VB2, VB6, pantothenic acid, etc. minerals Calcium hydrogen phosphate, stone powder, iron, copper, manganese, zinc, etc.

[0054] The guaranteed nutritional value of the above-mentioned feed is as follows:

[0055]

[0056]

[0057] All laboratory animals were cared for and handled in accordance with th...

Embodiment 1

[0059] A method for establishing a non-obese nonalcoholic fatty liver disease model in rats, comprising the steps of:

[0060] 1. Feed configuration: Prepare dry-fried soybeans. The dry-fried soybeans are prepared by Beijing Speifu Biotechnology Co., Ltd. The specific method is: dry-fry the soybeans with a temperature-controlled stir-fry machine, dry-fry them at 150°C for 15 minutes, and cool down to 70°C , pulverized; 60wt% of the above-mentioned dry-fried and pulverized soybeans were uniformly mixed with 40wt% of commercial standard rat feed to obtain human diet simulated feed.

[0061] 2. Establishment of rat model:

[0062] (1) Selection of experimental animals: 18 healthy male SD rats, 200-220g, 6-8 weeks, SPF grade;

[0063] (2) Experimental animal preparation: Maintain the experimental animals at 20-26°C for 2 weeks; feed them with a light / dark cycle of 12h:12h, and feed them with commercial standard rat feed and tap water. During the feeding period, the experimental a...

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Abstract

The invention discloses a method for establishing a non-obese non-alcoholic fatty liver disease model in rats. The human diet simulated feed containing high-temperature-treated soybeans is fed, and the high-temperature treatment is dry heat processing at 140-160°C for 12-18 minutes. . The invention adds high-temperature-processed soybeans to rat feed, and successfully constructs a non-obese non-alcoholic fatty liver disease model. The modeling method of the invention is simple, simulates the eating patterns of Chinese people, the rats are not obviously obese or thin, overcomes the defects of existing models, and provides a research basis for the pathogenesis of human non-obese non-alcoholic fatty liver. In addition, the pathological manifestation of the model of the present invention is mainly vesicular steatosis, which provides certain reference significance for the diagnosis of NAFLD.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for establishing a non-obese non-alcoholic fatty liver disease model in rats. Background technique [0002] Non-alcoholic fatty liver disease (NAFLD) refers to a clinicopathological syndrome characterized by diffuse hepatic fatty change caused by excluding alcohol and other definite liver damage factors. The disease spectrum includes Simple hepatic steatosis, non-alcoholic steatohepatitis (NASH), cirrhosis and hepatocellular carcinoma. NAFLD has now become one of the most common chronic liver diseases in the world, with a global prevalence of about 25.24% among adults. In 2018, the prevalence of NAFLD in my country was 29.6%. NAFLD has surpassed viral hepatitis to become the number one liver disease in my country, but there is still a lack of safe and effective therapeutic drugs for NAFLD in clinical practice. NAFLD not only brings serious economic burden to i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/02A23K10/30A23K50/50
CPCA01K67/02A23K10/30A23K50/50A01K2207/25A01K2227/105A01K2267/03
Inventor 薛丽君姚树坤李沣骥周元琛韩聚强张锋
Owner BEIHANG UNIV
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