Method for establishing non-obese rat model of non-alcoholic fatty liver disease

A technique for fatty liver disease and establishment of a method is applied in the field of establishment of a non-obese non-alcoholic fatty liver disease model in rats, which can solve the problems of incomplete research and incomplete elucidation of the mechanism of non-obese non-alcoholic fatty liver disease.

Active Publication Date: 2020-10-20
BEIHANG UNIV +2
View PDF4 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Research on non-obese nonalcoholic fatty liver disease is i

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for establishing non-obese rat model of non-alcoholic fatty liver disease
  • Method for establishing non-obese rat model of non-alcoholic fatty liver disease
  • Method for establishing non-obese rat model of non-alcoholic fatty liver disease

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach

[0049] Experimental animals were provided by Beijing Huafukang Biotechnology Co., Ltd.;

[0050] Soybean is Zhonghuang 13 non-transgenic soybean, purchased from Chinese Academy of Agricultural Sciences;

[0051] The inflammatory factor Elisa kit was purchased from Jitai Yikesai Company;

[0052] The commercial standard rat feed was purchased from Beijing Huafukang Biotechnology Co., Ltd., the name and model were rat maintenance feed 1022, and its ingredients were as follows:

[0053] protein source soybean meal, fish meal fat source vegetable oil fiber source bran carbohydrate source corn, flour vitamins VA, VD, VE, VB1, VB2, VB6, pantothenic acid, etc. minerals Calcium hydrogen phosphate, stone powder, iron, copper, manganese, zinc, etc.

[0054] The guaranteed nutritional value of the above-mentioned feed is as follows:

[0055]

[0056]

[0057] All laboratory animals were cared for and handled in accordance with th...

Embodiment 1

[0059] A method for establishing a non-obese nonalcoholic fatty liver disease model in rats, comprising the steps of:

[0060] 1. Feed configuration: Prepare dry-fried soybeans. The dry-fried soybeans are prepared by Beijing Speifu Biotechnology Co., Ltd. The specific method is: dry-fry the soybeans with a temperature-controlled stir-fry machine, dry-fry them at 150°C for 15 minutes, and cool down to 70°C , pulverized; 60wt% of the above-mentioned dry-fried and pulverized soybeans were uniformly mixed with 40wt% of commercial standard rat feed to obtain human diet simulated feed.

[0061] 2. Establishment of rat model:

[0062] (1) Selection of experimental animals: 18 healthy male SD rats, 200-220g, 6-8 weeks, SPF grade;

[0063] (2) Experimental animal preparation: Maintain the experimental animals at 20-26°C for 2 weeks; feed them with a light / dark cycle of 12h:12h, and feed them with commercial standard rat feed and tap water. During the feeding period, the experimental a...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for establishing a non-obese rat model of the non-alcoholic fatty liver disease. The method comprises a step of feeding with a human diet simulation feed containing soybeans having been subjected to high-temperature treatment, wherein the high-temperature treatment refers to dry hot processing at 140-160 DEG C for 12-18 min. The soybeans processed at a high temperature are added into the rat feed so as to successfully construct the non-obese rat model of the non-alcoholic fatty liver disease model. The modeling method disclosed by the invention is simple, simulates the diet mode of Chinese people, avoids obvious obesity or emaciation of rats, overcomes the defects of existing models, and provides a research basis for the pathogenesis of the non-alcoholic fatty liver disease in non-obesity human beings. In addition, the pathological manifestation of the model is mainly microvesicular lipid change, so certain reference significance is provided for diagnosis of NAFLD.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for establishing a non-obese non-alcoholic fatty liver disease model in rats. Background technique [0002] Non-alcoholic fatty liver disease (NAFLD) refers to a clinicopathological syndrome characterized by diffuse hepatic fatty change caused by excluding alcohol and other definite liver damage factors. The disease spectrum includes Simple hepatic steatosis, non-alcoholic steatohepatitis (NASH), cirrhosis and hepatocellular carcinoma. NAFLD has now become one of the most common chronic liver diseases in the world, with a global prevalence of about 25.24% among adults. In 2018, the prevalence of NAFLD in my country was 29.6%. NAFLD has surpassed viral hepatitis to become the number one liver disease in my country, but there is still a lack of safe and effective therapeutic drugs for NAFLD in clinical practice. NAFLD not only brings serious economic burden to i...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01K67/02A23K10/30A23K50/50
CPCA01K67/02A23K10/30A23K50/50A01K2207/25A01K2227/105A01K2267/03
Inventor 薛丽君姚树坤李沣骥周元琛韩聚强张锋
Owner BEIHANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap