Application of MicroRNA-455-3p in treatment of osteoarthritis

A technology for osteoarthritis and therapeutic targets, applied in the field of application of MicroRNA-455-3p in the treatment of osteoarthritis, can solve problems such as cartilage degeneration

Active Publication Date: 2020-10-20
THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Mesenchymal stem cells (mesenchymal stem cells, MSCs) have the ability to differentiate into chondrocytes, etc., but the cartilage induced by differentiation currently has the disadvantage of early degeneration of cartilage, so the solution to prevent cartilage degeneration and maintain cartilage stability The state becomes the key point to prevent the occurrence and development of OA

Method used

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  • Application of MicroRNA-455-3p in treatment of osteoarthritis
  • Application of MicroRNA-455-3p in treatment of osteoarthritis
  • Application of MicroRNA-455-3p in treatment of osteoarthritis

Examples

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Effect test

Embodiment 1

[0023] Example 1 Differentially expressed miR-455-3p was screened out from hADSCs during the induction of chondrogenic differentiation

[0024] During the differentiation of human adipose-derived stem cells (hADSCs) into cartilage, we found that miR-455-3p was significantly higher at 14 days of hADSCs-induced chondrogenic differentiation than at 0 days (3Versus 3) by using miRNA chip (average increase multiple: 2.973569), thus screening out the differentially expressed miR-455-3p, which suggests that miR-455-3p may be closely related to chondrogenic differentiation and found that miR-455-3p through histone The regulation of acetylation and DNA methylation promotes cartilage differentiation and delays cartilage degeneration, especially can significantly promote the differentiation of hADSCs into cartilage and inhibit cartilage degeneration.

Embodiment 2

[0025] Example 2 Animal model verification of relationship between deletion of miR-455-3p and OA progression

[0026] Construction of miR-455-3p knockout mice

[0027] We use the Transcription Activator-Like Effector Nuclease (TALEN) technology system to interrupt the endonuclease activity of FokI to disrupt the target gene miR-455-3p. FokI forms a dimer and exerts its activity. Under the guidance of the TALEN binding arm, it exerts specific endonuclease activity, cleaves DNA between two target sites, forms a DNA double-strand break (DSB), and induces a DNA damage repair mechanism. . Cells repair DNA damage through non-homologous end joining (NHEJ). During this modification process, a certain number of bases (not multiples of 3) are deleted or inserted, resulting in a frame shift and forming a specific gene for the target gene miR-455-3p. Sexual KO mutants. We have successfully constructed miR-455-3p gene knockout (KO) mice, and have obtained stable bred miR-455-3p(- / -)C5...

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Abstract

The invention provides an application of MicroRNA-455-3p as an osteoarthritis treatment target and an application of an exosome for overexpression of MicroRNA-455-3p in preparation of a drug for treating osteoarthritis. According to the invention, the MicroRNA-455-3p is used as an osteoarthritis treatment target, and the exosome vector for overexpression of the MicroRNA-455-3p is constructed to carry out early intervention on osteoarthritis so as to realize the purpose of treating OA.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to the application of MicroRNA-455-3p in the treatment of osteoarthritis. Background technique [0002] Osteoarthritis (OA) is the most common chronic joint disease. Lancet et al. reported that about 250 million people around the world suffer from OA. The aging society and the lack of effective early intervention have caused OA to cause more and more heavy social and economic costs. burden. The current treatment methods for OA are mainly anti-inflammatory and analgesic drugs (NSAID) and glucosamine and other symptomatic treatments in the early stage, intra-articular injection of sodium hyaluronate, platelet-rich plasma (PRP) treatment, and rehabilitation physiotherapy. Endoscopic debridement often requires joint replacement in the advanced stage. [0003] Since the pathogenesis and therapeutic targets of OA have not yet been fully clarified, NSAIDs, glucosamine, so...

Claims

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Application Information

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IPC IPC(8): A61K45/00A61K48/00A61P19/02A61P29/00C12Q1/6883
CPCA61K45/00A61P19/02A61P29/00A61K48/0066A61K48/0008A61K48/0075C12Q1/6883C12Q2600/158C12Q2600/178
Inventor张志奇廖威明赵潇艺利洪艺文星钊孙浩陈蔚深胡舒毛谷平江宗睿杜雪
OwnerTHE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV