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Application of L-alanine in preparation of medicine for preventing and treating tuberculosis

A technology of alanine and drugs, applied in the field of application of L-alanine in the preparation of drugs for the prevention and treatment of tuberculosis, which can solve the problems of little knowledge about the impact of tuberculosis disease outcome

Inactive Publication Date: 2020-10-23
SHANGHAI PULMONARY HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in tuberculosis caused by Mycobacterium tuberculosis infection, small molecular metabolites have been used in the research of disease diagnosis, and little is known about their impact on the outcome of tuberculosis

Method used

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  • Application of L-alanine in preparation of medicine for preventing and treating tuberculosis
  • Application of L-alanine in preparation of medicine for preventing and treating tuberculosis
  • Application of L-alanine in preparation of medicine for preventing and treating tuberculosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Infection models of C57BL / 6 mice and SCID mice were used to analyze the effect of L-alanine on tuberculosis.

[0025] (1) C57BL / 6 mouse infection model experiment: wild-type 6-8-week-old C57BL / 6 female mice, 8 in each group, were infected with Mycobacterium tuberculosis H37Rv bacteria through the respiratory tract, about 200 CFU / mouse. The control group was given drinking water, the L-alanine group added L-alanine (30mg / mL) to the drinking water, and the D-alanine group added D-alanine (30mg / mL) to the drinking water as a drug control. ), the mice were sacrificed by neck dislocation 30 days after infection, and the lung tissue was isolated to detect the following indicators: 1) Lung CFU: the lung tissue was collected under sterile conditions, diluted 10 times and 100 times after homogenization, and each 0.1ml was inoculated in Michaelis 7H10 medium was cultured at 37°C for 4 weeks, the growth of MTB was observed, the number of colonies (CFU) was counted, and the bacteri...

Embodiment 2

[0029] In vitro infection model of macrophages to analyze the effect of L-alanine on the immune function of macrophages.

[0030] (1) The effect of antimicrobial peptides: Take mouse peritoneal macrophages or mouse immortalized bone marrow-derived macrophages (Immortalized bone marrow-derived macrophages, iBMDM), add L at final concentrations of 0, 0.1 and 1 mM - H37Rv infection was administered 12 hours after alanine treatment, MOI=5. After 0, 12, and 24 hours of infection, remove the supernatant, lyse the cells with 1ml TRIZOL, extract RNA, detect the changes in the expression levels of antimicrobial peptides and other related molecules by RT-PCR, and determine the effect of L-alanine on the production of antimicrobial peptides in macrophages. Impact. Experimental results such as image 3 shown.

[0031] (2) Intracellular survival experiment: take the primary macrophages in the peritoneal cavity of wild-type mice, culture them with complete 1640 medium (containing 10% FBS...

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Abstract

The invention discloses application of L-alanine in preparation of a medicine for preventing and treating tuberculosis. By means of a C57BL / 6 mouse and SCID mouse infection model, a macrophage in-vitro infection model, an exogenous metabolite adding mode and the like, it is proved that L-alanine resists tubercle bacillus infection through natural immune cell protection host, L-alanine promotes macrophage antibacterial peptide generation and limits tubercle bacillus intracellular survival; and the L-alanine is expected to assist the current antituberculous drugs, can shorten the course of treatment and improve the curative effect, and provides reference for clinical treatment.

Description

technical field [0001] The invention relates to the field of tuberculosis prevention and control, in particular to the application of L-alanine in the preparation of drugs for preventing and treating tuberculosis. Background technique [0002] Tuberculosis caused by Mycobacterium Tuberculosis (MTB) infection is one of the top ten causes of death in the world, and it is also the infectious disease that causes the largest number of deaths. my country is also in a severe situation and is the country with the second highest burden of tuberculosis in the world. The recommended treatment plan for tuberculosis is to use multiple anti-tuberculosis drugs in combination for 6-9 months, which leads to poor drug compliance of patients and further promotes the development of multidrug-resistant tuberculosis (MDR-TB). At present, MDR-TB has become a threat to global public health. The cure rate of MDR-TB patients after treatment with conventional regimens is less than 50%, and some patie...

Claims

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Application Information

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IPC IPC(8): A61K31/198A61P31/06A61P11/00
CPCA61K31/198A61P11/00A61P31/06
Inventor 戈宝学王琳彭程
Owner SHANGHAI PULMONARY HOSPITAL
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