Preparation method of tissue engineered bone with regeneration ability

A technology of tissue engineering bone and regenerative ability, applied in the field of medical materials, can solve the problems such as difficulty in angiogenesis, bone marrow reconstruction and formation of blood vessels, and achieve the effects of avoiding the synchronization of body growth and degeneration, low cost, and ensuring nutrition supply

Active Publication Date: 2020-10-27
弗元(上海)生物科技有限公司 +1
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the development of technology, absorbable and tissue-compatible materials are also used in the treatment of bone defects, but at present, it is difficult for tissue engineering materials and tissue-engineered bone to form blood vessels, and to form a balanced state of bone formation and resorption, and regenerate tissue There are still great differences in structure and function from normal tissues, and it is difficult to complete angiogenesis and bone marrow reconstruction in the treatment of bone loss, and cannot completely replace the missing function

Method used

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Embodiment Construction

[0020] The present invention will be described in detail below in combination with specific embodiments.

[0021] The embodiment of the present invention discloses a preparation method of tissue engineered bone with regenerative ability, comprising the following steps:

[0022] S1. Preparation of collagen matrix: Add 200 mg of sterile, biologically active and undenatured type I collagen, type II collagen or composite type collagen into a 5 mL sterile tube, and add low-sugar pre-cooled at 4 °C to it. 1mL of DMEM medium, after being fully mixed at 4°C, let it stand for later use;

[0023] S2. Preparation of mesenchymal stem cells: select human mesenchymal stem cells in good condition obtained from patient fat 7 cells / cm 2 The concentration is suspended in the induction medium to obtain a cell suspension;

[0024] S3. Preparation of bone marrow: extract human bone marrow with EDTA anticoagulant to obtain fresh bone marrow, suspend the fresh bone marrow with 5 times the amount ...

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Abstract

The invention discloses a preparation method of tissue engineered bone with a regeneration ability. The preparation method comprises the following steps of S1, preparation of collagen matrix, S2, preparation of mesenchymal stem cells, S3, preparation of bone marrow, S4, cultivation and preparation of semi-finished tissue engineered bone and S5, cultivation and preparation of the tissue engineeredbone with the regeneration ability. The method is short in osteogenic induction time, high in safety, low in cost, and suitable for simple bone tissue production, the prepared tissue engineered bone can be completely fused with body tissues after being embedded in a body and then grow into a part of the body, so that a purpose of tissue regeneration is achieved, the problem that fixed tissue materials cannot be synchronized with the growth and degeneration of the body is completely solved, and the tissue engineered bone can further be shaped, spliced and matured in the body and is an ideal regenerative medicine final product and material product.

Description

technical field [0001] The invention relates to the technical field of medical materials, in particular to a method for preparing tissue-engineered bone with regenerative capacity. Background technique [0002] Due to certain factors such as trauma, infection, tumor resection or congenital diseases, the bone loses some bone mass and forms a large gap, which is called bone defect. Bone defects are relatively common clinically, and their treatment has always been a difficult point in clinical treatment. Bone transplantation provides the possibility to solve the above problems. At present, autologous bone is still the first choice of bone graft source, but its supply is limited, and it is prone to the dilemma of "removing the east wall to fill the west wall" in application, which is difficult to meet the clinical needs. Therefore, finding a suitable bone graft substitute has become the primary problem in the research and repair of bone defects. [0003] Tissue engineering bon...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38A61L27/36A61L27/24A61L27/50
CPCA61L27/3834A61L27/3847A61L27/3608A61L27/3687A61L27/3691A61L27/365A61L27/24A61L27/50A61L2430/02
Inventor 李文放董永聘江伟伟冯荣
Owner 弗元(上海)生物科技有限公司
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