NK cell low-temperature preservation re-infusion liquid

Abstract

The invention provides an NK cell low-temperature preservation re-infusion liquid. According to the invention, a glucose and sodium chloride injection is added into the NK cell low-temperature preservation re-infusion liquid to serve as a diluent / buffer liquid, so that the effects of maintaining the pH value, the ion osmotic pressure and the colloid osmotic pressure and providing energy glucose for cells are achieved; the added human serum albumin can maintain the constant osmotic pressure of plasma colloid and supply nutrition; the added vitamin C can promote antibody and collagen formation and tissue repair, and meanwhile, the vitamin C also has the effects of resisting oxidation, resisting free radicals and inhibiting tyrosinase formation, so that the oxidative damage of cells can be reduced, and the accumulation of peroxides in the cells can be quickly relieved. According to the NK cell low-temperature preservation re-infusion liquid, the survival rate of NK cells can be kept at 80% or above after the NK cells are preserved for 48 h at the temperature of 4 DEG C, and a preparation can be directly re-infused.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a low-temperature preservation reinfusion solution of NK cells. Background technique [0002] Natural killer cells (Natural Killer Cell, NK) are important immune cells in the body, not only related to anti-tumor, anti-viral infection and immune regulation, but also involved in the occurrence of hypersensitivity and autoimmune diseases in some cases, and can recognize Target cells, killing mediators. [0003] Generally, the way of reinfusion of NK cells is to use physiological saline with human serum albumin as the reinfusion solution of the cells, but due to the lack of nutrition and buffer capacity, the cell activity declines rapidly, and the reinfusion can only be performed in a short time. The activity of cells cannot be guaranteed when transported in different places. [0004] In order to improve cell viability, ultra-low temperature cryopreservation preparations are used for s...

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Problems solved by technology

[0005] The main purpose of the present invention is to provide a NK cell cryopreservation reinfusion solution, which aims to solve the problems of reduced cell activity, high cost of ultra-low temperature transportation, and cumbersome operation during the long-term transportation of NK cells in different places, so that it can be stored at room temperature or low temperature. Maintain cell viability for a longer period of time under certain conditions, reducing costs

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