NK cell low-temperature preservation re-infusion liquid

A low-temperature preservation and NK cell technology, applied in the biological field, can solve the problems of cumbersome operation, high cost, and reduced cell activity

Inactive Publication Date: 2020-10-30
SHENZHEN PREGENE BIOPHARMA CO LTD
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

[0005] The main purpose of the present invention is to provide a NK cell cryopreservation reinfusion solution, which aims to solve the problems of reduced cell activity, high cost of ultra-low temperature transportation, and cumberso...

Method used

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  • NK cell low-temperature preservation re-infusion liquid
  • NK cell low-temperature preservation re-infusion liquid
  • NK cell low-temperature preservation re-infusion liquid

Examples

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Embodiment 1

[0033] Example 1 Design of different NK cells cryopreserved and returned to infusion

[0034] According to the main function and required content of each component in the low-temperature preservation reinfusion formula in the technical scheme of the present invention, formulate A, B, C, D four kinds of low-temperature preservation reinfusion formulas, each preparing 200ml, in each formula, human serum albumin The volume concentration of human serum albumin in the injection is 20%, the content of vitamin C in the vitamin C injection is 25%, the mass concentration of glucose in the glucose sodium chloride injection is 5%, the human serum albumin injection, vitamin The usage ratio and dosage of C injection and glucose sodium chloride injection are shown in Table 1. According to the formulations in Table 1, four kinds of NK cell cryopreservation back infusions A, B, C, and D were prepared respectively.

[0035] Table 1. Different NK cell cryopreservation back infusion formulations...

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Abstract

The invention provides an NK cell low-temperature preservation re-infusion liquid. According to the invention, a glucose and sodium chloride injection is added into the NK cell low-temperature preservation re-infusion liquid to serve as a diluent/buffer liquid, so that the effects of maintaining the pH value, the ion osmotic pressure and the colloid osmotic pressure and providing energy glucose for cells are achieved; the added human serum albumin can maintain the constant osmotic pressure of plasma colloid and supply nutrition; the added vitamin C can promote antibody and collagen formation and tissue repair, and meanwhile, the vitamin C also has the effects of resisting oxidation, resisting free radicals and inhibiting tyrosinase formation, so that the oxidative damage of cells can be reduced, and the accumulation of peroxides in the cells can be quickly relieved. According to the NK cell low-temperature preservation re-infusion liquid, the survival rate of NK cells can be kept at 80% or above after the NK cells are preserved for 48 h at the temperature of 4 DEG C, and a preparation can be directly re-infused.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a low-temperature preservation reinfusion solution of NK cells. Background technique [0002] Natural killer cells (Natural Killer Cell, NK) are important immune cells in the body, not only related to anti-tumor, anti-viral infection and immune regulation, but also involved in the occurrence of hypersensitivity and autoimmune diseases in some cases, and can recognize Target cells, killing mediators. [0003] Generally, the way of reinfusion of NK cells is to use physiological saline with human serum albumin as the reinfusion solution of the cells, but due to the lack of nutrition and buffer capacity, the cell activity declines rapidly, and the reinfusion can only be performed in a short time. The activity of cells cannot be guaranteed when transported in different places. [0004] In order to improve cell viability, ultra-low temperature cryopreservation preparations are used for s...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0226
Inventor 宋宗培张珊罗燕平包朝乐萌何嵘刘丽美
Owner SHENZHEN PREGENE BIOPHARMA CO LTD
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