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A Large Field of View Digital Scanning Light Sheet Illumination System Based on Phase Conjugate Mirrors

A digital scanning and lighting system technology, applied in optics, microscopes, optical components, etc., can solve the problem of not being able to real-time imaging, and achieve the effect of improving energy utilization, improving fluorescence excitation efficiency, and improving imaging contrast.

Active Publication Date: 2022-08-02
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This method requires subsequent image processing and cannot be imaged in real time

Method used

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  • A Large Field of View Digital Scanning Light Sheet Illumination System Based on Phase Conjugate Mirrors
  • A Large Field of View Digital Scanning Light Sheet Illumination System Based on Phase Conjugate Mirrors
  • A Large Field of View Digital Scanning Light Sheet Illumination System Based on Phase Conjugate Mirrors

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Embodiment Construction

[0031] The technical content of the present invention is specifically described below by citing examples and in conjunction with the listed drawings, as follows:

[0032] like figure 1 As shown, a large field of view digital scanning light sheet illumination system based on a phase conjugate mirror, adding a laser gain medium and a self-pumping phase conjugate mirror to the other end of the illumination optical path of a traditional light sheet microscope, coaxial with the illumination optical path , the excitation light signal transmitted through the sample cell will form a phase conjugate light wave with gain of the excitation light. The phase-conjugated light wave with gain is time-reversed with the incident excitation light, and returns to the sample cell again with the same waveform of the incident light, forming an overlapping light sheet with the incident light to excite the fluorescence information of the sample. Compared with the traditional light sheet illumination ...

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Abstract

The invention proposes a large field of view digital scanning light sheet illumination system based on a phase conjugate mirror, including a light source module, a spatial light modulation module, a scanning module, an illumination module and a phase conjugate module, the illumination module and the phase conjugate module Coaxially arranged, the illumination module includes an illumination objective lens and a sample cell arranged in sequence, the phase conjugate module includes a laser amplifier, an optical phase conjugate mirror and a first mirror arranged in sequence, and the light emitted by the light source module is reflected to the spatial light modulation module. After passing through the scanning module, it is incident on the entrance pupil surface of the illumination objective lens of the illumination module and then focused in the sample cell to form a thin slice of light, which excites the fluorescence signal, the transmitted light passes through the laser amplifier, and the gain transmitted light passes through the optical phase conjugate mirror to generate incident light along the incident light. The phase-conjugated reflected light wave of the light is amplified by the laser amplifier again, and returns to the sample cell to focus to form a thin excitation light sheet overlapping with the incident light sheet.

Description

technical field [0001] The invention belongs to the technical field of optical microscopic imaging, and in particular relates to a large field of view digital scanning light sheet illumination system based on a phase conjugate mirror. Background technique [0002] The traditional light sheet microscope illumination light path and imaging detection light path are perpendicular to each other, and the illumination light is scanned by a cylindrical lens or a galvanometer to form a thin sheet of light near the back focal plane of the illumination objective lens. Only the fluorescence information is excited within the slice, and the resolution and contrast are significantly improved relative to epifluorescence microscopy. Traditional light-sheet microscopes limit the development of light-sheet microscopes due to the contradiction between resolution and field of view, imaging speed and phototoxicity. Most of the illumination schemes used by existing light sheet microscopes are Gau...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G02B21/00
CPCG02B21/0072G02B21/0076
Inventor 王吉祥张欣徐欣吴越前吴爽史国华
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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