A kind of 5-alkylthiazolamine derivative and its antidepressant use
A technology of aniline and compounds, applied in the field of medicinal chemistry, to achieve good antidepressant activity, mild conditions, and simple preparation routes
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Embodiment 13
[0039] Example 13-[(5-ethyl-4-(3,4-difluorophenyl)thiazol-2-yl)amino]benzoic acid
[0040] Step 1: 3-[(-4-phenylthiazol-2-yl)amino]benzoic acid
[0041]
[0042] 2.12g (0.01mol) 3-carboxyphenylthiourea, 2.49g (0.01mol) 2-bromo-1-(3,4-difluorophenyl)acetone and 15 mL of glacial acetic acid, stirred evenly, heated to reflux, TLC (developing solvent: ethyl acetate: petroleum ether=4:1) monitored the reaction progress, and the reaction was carried out for about 24 hours. Remove the insoluble solids in the reaction flask while it is still hot, and rotate part of the solvent. It was put into a fume hood to cool at room temperature, the solid was precipitated, filtered, and the obtained solid was dried and weighed to obtain 2.09 g of powder with a yield of 60.4%. 1 H NMR (DMSO-D 6 , 400MHz), δ: 2.43 (s, 3H, CH 3 ), 7.31-8.26 (m, 7H, C 6 H 4 , C 6 H 3 ), 10.35 (s, 1H, COOH).
[0043] Step 2: Preparation of the compound of formula I
[0044]
[0045] Add (0.28g, 3mmol) ...
Embodiment 2
[0047] Example 2 Antidepressant activity test
[0048] In this experiment, the high-throughput selection method of fluorescent substance ASP is used to find compounds that directly act on or produce reuptake inhibitory effects through adenosine receptors. Fluorescent dye ASP+ is a neurotoxic compound that can bind to monoamine transporters into cells and emit yellow fluorescence (the order of binding is dopamine transporter>adrenaline transporter>5-HT transporter). When there are other compounds that can bind to the transporter, it competes with ASP, thereby reducing the number of cells entering the cell, and the yellow fluorescence is weakened.
[0049] RBL cells are a cell line that can secrete histamine and 5-HT, and the cells are immortalized mast cells that can release the transmitters quantumly. And has the ability to re-uptake 5-HT.
[0050] CACO-2 cells are adenocarcinoma cell lines derived from human small intestine. Studies have shown that after a period of culture...
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