Mass spectrum imaging device and method under vacuum

Abstract

The invention discloses a mass spectrum imaging device and method under vacuum. The device comprises a vacuum cavity; an ion guiding device which is located in the vacuum cavity and defines a space extending in the axis direction, wherein an outlet part of the space is communicated with a post-stage device; a power supply device which is used for forming an electric field in the ion guide device and at least can be used for guiding ions to the outlet part to the post-stage device; and a sample stage which carries a sample and is positioned in the vacuum cavity and at the inlet part or the outlet part; the laser irradiates the sample to generate ions to be analyzed to enter the ion guide device. The device extends in the axial direction, but the cross section is kept unchanged, so that large-cross-section receiving of ions is guaranteed; a light path can penetrate through the space along the axial direction to ensure an extremely small incident angle, so that high sensitivity and high spatial resolution are obtained; due to the characteristics of deflection focusing and vertical extraction, ion transmission is separated from a light path, and secondary action of laser and ions is avoided; and the device can be combined with other ion sources for use through an atmospheric pressure sample injection interface.

Description

technical field [0001] The present application relates to the technical field of mass analysis, in particular to a mass spectrometry imaging device and method under vacuum. Background technique [0002] In mass spectrometry imaging (mass spectrometry imaging) technology, especially in mass spectrometry imaging using MALDI (matrix-assisted laser desorption ionization) as an ion source, usually MALDI works in a relatively high vacuum below 10-4Pa, and the laser beam bombards The surface of the thin sample is pre-treated with the matrix, so that the analyte on the sample surface is ionized to generate ions, and the ions enter the mass analyzer for mass analysis. The so-called mass spectrometry imaging is to place a sample (such as a biological tissue sample) on a two-dimensional mobile platform. By moving the sample, the laser scans different positions of the sample point by point, and each position forms at least one mass spectrum. Finally, the These spectrogram data are aggr...

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Problems solved by technology

[0004] In view of the shortcomings of the prior art described above, the purpose of this application is to provide a mass spectrometry imaging device and method under vacuum, aiming to solve the inconvenience and limitations of sample processing in the prior art devices that use MALDI ion sources for mass spectrometry imaging. Various problems such as complex structure and possible destruction of desired ions

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