Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for evaluating activity of immune cells after cryopreservation

An immune cell and cryogenic technology, applied in the field of biotechnology, can solve problems such as unreliable results and effects on immune cell activity, and achieve intuitive and fast evaluation results.

Pending Publication Date: 2020-11-17
赛济检验认证中心有限责任公司
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the activity of immune cells after cryopreservation may be affected. If immune cells with unknown cell activity are used for treatment or clinical trials, the results are unreliable

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for evaluating activity of immune cells after cryopreservation
  • Method for evaluating activity of immune cells after cryopreservation
  • Method for evaluating activity of immune cells after cryopreservation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Detection of NK cells before cryopreservation

[0027] Detection steps:

[0028] 1. Take five 1.8ml EP tubes, numbered 1, 2, 3, 4, and 5 respectively.

[0029] 2. Mix the cells to be detected, take 1ml of the cell solution into No. 1 EP tube, mix and count.

[0030] 3. Take No. 2, No. 3, No. 4, and No. 5 EP tubes, and add 500ul of normal saline to each EP tube.

[0031] 4. Take 500ul of cell liquid from No. 1 EP tube and put it into No. 2 EP tube, and mix well.

[0032] 5. Take 500ul of cell liquid from No. 2 EP tube and put it into No. 3 EP tube, and mix well.

[0033] 6. Take 500ul of cell liquid from No. 3 EP tube and put it into No. 4 EP tube, and mix well.

[0034] 7. Take 500ul of cell liquid from No. 4 EP tube and put it into No. 5 EP tube, and mix well.

[0035] 8. Take 50ul of cell solution from EP tubes 1, 2, 3, 4, and 5 and place them in 96-well opaque white plates, with 3 replicate wells for each group.

[0036] 8. Add 50ul of prepared CellT...

Embodiment 2

[0038] Example 2: Fluorescence monitoring of revived NK cells.

[0039] Detection steps:

[0040] 1. Take five 1.8ml EP tubes, numbered 1, 2, 3, 4, and 5 respectively.

[0041] 2. Mix the cells to be detected, take 1ml of the cell solution into No. 1 EP tube, mix and count.

[0042] 3. Take No. 2, No. 3, No. 4, and No. 5 EP tubes, and add 500ul of normal saline to each EP tube.

[0043] 4. Take 500ul of cell liquid from No. 1 EP tube and put it into No. 2 EP tube, and mix well.

[0044] 5. Take 500ul of cell liquid from No. 2 EP tube and put it into No. 3 EP tube, and mix well.

[0045] 6. Take 500ul of cell liquid from No. 3 EP tube and put it into No. 4 EP tube, and mix well.

[0046] 7. Take 500ul of cell liquid from No. 4 EP tube and put it into No. 5 EP tube, and mix well.

[0047] 8. Take 50ul of cell solution from EP tubes 1, 2, 3, 4, and 5 and place them in 96-well opaque white plates, with 3 replicate wells for each group.

[0048] 8. Add 50ul of prepared CellTi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for evaluating the activity of immune cells after cryopreservation, which is characterized by comprising a test reagent reaction solution preparation step and a cell sap dilution detection step. According to the method, the ATP value of the resuscitated immune cells can be detected through fluorescence, so that the cell activity of the immune cells after cryopreservation can be quickly and visually evaluated, so that the evaluation is more intuitive and faster.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for evaluating the activity of immune cells after cryopreservation. Background technique [0002] In recent years, many immunotherapies for treating various diseases have been established, and it is necessary to grow high-quality immune cells. Patients with various diseases have an increasing demand for immune cells. We must cryopreserve immune cells so that they can supply patients' needs for immune cells at any time after recovery. [0003] However, the activity of immune cells after cryopreservation may be affected. If immune cells with unknown cell activity are used for treatment or clinical trials, the results will be unreliable. Therefore, it is necessary to evaluate the cellular viability of immune cells after cryopreservation. Contents of the invention [0004] In order to overcome the above-mentioned defects of the prior art, the object of the present invention ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76G01N21/64G01N1/38
CPCG01N21/76G01N21/6486G01N1/38
Inventor 雷颖王瑛胡晓婷
Owner 赛济检验认证中心有限责任公司