Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Fluorescence immunochromatography detection method and test paper and application thereof

A technology of fluorescent immunochromatography and detection test paper, which is applied in the direction of measuring devices, analysis materials, instruments, etc., can solve the problems of detection interference and interference results, etc., and achieve the effect of reducing interference factors, reducing interference substances, and improving accuracy

Pending Publication Date: 2020-11-20
SHANGHAI RONGSHENG BIOLOGICAL PHARM CO LTD
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This kind of one-way chromatography with one sample (i.e. "one-step method") interferes more with the detection, especially in the one-step reaction implemented by the indirect method, the same detection antibody (such as IgG antibody) carried by the sample (such as: blood) may bind to the microspheres and interfere with the results

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fluorescence immunochromatography detection method and test paper and application thereof
  • Fluorescence immunochromatography detection method and test paper and application thereof
  • Fluorescence immunochromatography detection method and test paper and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] figure 1 Fluorescence immunochromatography test paper, including dilution pad 600 and absorbent paper 100, respectively placed on both ends of the test paper, from the dilution pad 600 to the direction of absorbent paper 100, fluorescent pad 500, instant film 400, sample The pad 300 and the NC film 200, and the T line 210 and the C line 220 are arranged on the NC film 200, and the fluorescent microspheres (not shown) are placed on the fluorescent pad 500.

[0024] In use, the sample solution is added to the sample pad 300 , and the diluent is added to the dilution pad 600 . After the sample liquid is added, on the one hand, during the chromatographic process of the sample liquid toward the T-line 210 , the substance to be tested therein is fixed at the T-line 210 . On the other hand, due to the effect of liquid diffusion, a small part of the sample solution and diluent wet and dissolve the instant film 400, so that the diluent drives the fluorescent microspheres in the...

Embodiment 2

[0028] Commercially available test strips and the test strips of this example were used to detect low-concentration hepatitis B surface antibody-positive serum samples with a concentration of 20 mIU, and the specific test results are shown in Table 1.

[0029] Table 1

[0030]

Embodiment 3

[0032] Commercially available test strips and the test strips of this example were used to detect low-concentration hepatitis B surface antibody-positive serum samples with a concentration of 50 mIU, and the specific test results are shown in Table 2.

[0033] Table 2

[0034]

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Thicknessaaaaaaaaaa
Thicknessaaaaaaaaaa
Molecular weightaaaaaaaaaa
Login to View More

Abstract

Disclosed is a fluorescence immunochromatography detection method. A to-be-detected sample solution and a sample diluent are respectively added into a sample pad and a dilution pad, chromatography onthe sample solution towards a T line is carried out under the capillary action, and a to-be-detected object is combined with the T line. Under the action of liquid diffusion, after the sample solutiondissolves an instant film between a sample pad and a fluorescent pad, the fluorescent microspheres carried by the diluent are subjected to chromatography towards the T line together and are combinedwith the sample at the T line, and finally, the fluorescence value at the T line is obtained and serves as the basis of sample detection. Tests prove that by adopting the detection method and the testpaper provided by the invention, results obtained in hepatitis B surface antibody detection show that the variable coefficient (CV) of T / C is remarkably reduced, interference factors are controlled,and the accuracy of fluorescence chromatography detection is remarkably improved.

Description

technical field [0001] The invention relates to a composition, in particular to a composition for fluorescence detection reagents, so as to improve the precision and accuracy of reagents for antibody detection. Background technique [0002] Fluorescence immunochromatography is a new type of membrane detection technology based on antigen-antibody specific immune response. In this technology, the strip-shaped fiber chromatography material immobilized with detection lines (coated antibodies or coated antigens) and quality control lines (anti-antibodies) is used as the stationary phase, the test solution is used as the mobile phase, and fluorescently labeled antibodies or antigens are fixed on the connection pads. , to move the analyte across the strip by capillary action. For macromolecular antigens (proteins, viruses, pathogenic bacteria, etc.) with multiple antigenic determinants, a "sandwich" type double-antibody sandwich immunochromatography method is usually used, that is...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/576G01N33/558
CPCG01N33/5764G01N33/558Y02A50/30
Inventor 杨永伟朱绍荣张成林
Owner SHANGHAI RONGSHENG BIOLOGICAL PHARM CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com