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Method for producing conjugated linoleic acid by adsorbing and immobilizing permeabilized lactic acid bacteria to transform rapeseed oil waste

A conjugated linoleic acid, adsorption and immobilization technology, applied in microorganism-based methods, biochemical equipment and methods, immobilized on or in inorganic carriers, etc., can solve complex products, long growth cycle, difficult separation and extraction, etc. problems, to achieve the effect of high transformed cell density, less interfering substances, and low cost of raw materials

Active Publication Date: 2017-12-01
常州凯康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional biological fermentation method has problems such as it is difficult to achieve high cell density, long growth cycle, low conversion rate, easy to infect bacteria, complex products, difficult to separate and extract, and bacteria cannot be reused.

Method used

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  • Method for producing conjugated linoleic acid by adsorbing and immobilizing permeabilized lactic acid bacteria to transform rapeseed oil waste

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1) Pretreatment of the adsorption and immobilization carrier: Crush the waste ceramics to obtain porous ceramic particles with a diameter of 4-6 mm. Soak in NaOH solution for 24 h, and finally wash with water until neutral, dry, and sterilize to obtain a sterile adsorption immobilized porous ceramic carrier.

[0017] (2) Preparation of permeabilized lactic acid bacteria: Pick 3-ring Lactobacillus acidophilus CGMCC1.1854 from fresh slant culture medium, inoculate it into 150 mL MRS medium, and incubate anaerobically at 37°C for 20 hours to obtain a lactic acid bacteria suspension , Take the bacterial suspension and continue to expand the culture in the MRS medium. Centrifuge the bacterial suspension at 4°C and 5000 rpm for 20 min, remove the supernatant, freeze the bacterial cells at -20°C, take them out, thaw them at room temperature, and repeat freezing and thawing twice to obtain permeabilized lactic acid bacteria.

[0018] (3) Adsorption and immobilization of perme...

Embodiment 2

[0022] (1) Pretreatment of adsorption and immobilization carrier: Crush waste ceramics to obtain porous ceramic particles with a diameter of 4-6 mm. Soak in NaOH solution for 24 h, and finally wash with water until neutral, dry, and sterilize to obtain a sterile adsorption immobilized porous ceramic carrier.

[0023] (2) Preparation of permeabilized lactic acid bacteria: Pick 2-ring Lactobacillus acidophilus CGMCC1.1854 from the fresh slant medium, inoculate it into 100 mL MRS medium, and culture it anaerobically at 35°C for 24 hours to obtain a lactic acid bacteria suspension , Take the bacterial suspension and continue to expand the culture in the MRS medium. Centrifuge the bacterial suspension at 4°C and 5000 rpm for 20 min, remove the supernatant, freeze the bacterial cells at -20°C, take them out, thaw them at room temperature, and repeat freezing and thawing 3 times to obtain permeabilized lactic acid bacteria.

[0024] (3) Adsorption and immobilization of permeabilized...

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Abstract

The invention relates to a method for producing conjugated linoleic acid by converting rape oil leftovers with adsorptive-immobilized permeabilized lactobacillus, which comprises the following steps: (1) pretreatment of adsorptive-immobilization carrier; (2) preparation of permeabilized lactobacillus; (3) adsorptive-immobilization of permeabilized lactobacillus; (4) emulsification and enzymolysis treatment on rape oil leftovers; and (5) conversion of rape oil leftover emulsification and enzymolysis solution with adsorptive-immobilized permeabilized lactobacillus. The method can recover the agricultural product waste rape oil leftovers and waste ceramics, and has the advantages of low raw material cost, high transformant density, high conversion rate (up to 40-60%, which is about 20% higher than that of the free permeabilized lactobacillus), low tendency to microbiological contamination in the conversion process, fewer interfering materials, easy product separation, recoverable thallus and the like.

Description

technical field [0001] The invention belongs to the technical field of agricultural product waste utilization. Background technique [0002] Conjugated linoleic acid (CLA), a natural active substance mainly found in ruminant fat and milk products, is a class of octadecadienoic acid (linoleic acid) containing conjugated double bonds The mixture of isomers, especially c-9, t-11 CLA and t-10, c-12 CLA, is considered to be the most biologically active, of which c-9, t-11 CLA is the only one that can be absorbed into phospholipids by animal cells layer of conjugated linoleic acid isomers, and t-10, c-12 CLA play an important role in anti-cancer, anti-atherosclerosis, immune function regulation. At present, expensive raw materials such as sunflower oil and safflower oil are usually used as substrates to produce conjugated linoleic acid. Rapeseed oil is one of the main edible oils in my country, and its total output accounts for more than 1 / 3 of the total output of vegetable oil....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/64C12N11/14C12R1/225
CPCC12N11/14C12P7/6427
Inventor 巫小丹徐尔尼徐颖宣刘志凤杨欣胡冰彬范相振黎紫含
Owner 常州凯康生物科技有限公司
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