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High-temperature-resistant high-carbohydrate-yield thermal-spring cyanobacteria and application thereof

A carbohydrate and high-temperature tolerance technology, applied in the direction of bacteria, microbe-based methods, microbes, etc., can solve problems such as research lag, achieve wide tolerance range, maintain high-speed growth, and reduce the effect of possibility

Active Publication Date: 2020-11-27
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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Problems solved by technology

However, due to the relatively lagging research on the isolation and purification of hot spring microorganisms, the development of "temperature-type" microalgae resources in terrestrial hot springs still has room for development

Method used

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  • High-temperature-resistant high-carbohydrate-yield thermal-spring cyanobacteria and application thereof
  • High-temperature-resistant high-carbohydrate-yield thermal-spring cyanobacteria and application thereof
  • High-temperature-resistant high-carbohydrate-yield thermal-spring cyanobacteria and application thereof

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Embodiment Construction

[0027] The principles and features of the present invention are described below in conjunction with examples, which are only used to explain the present invention and are not intended to limit the scope of the present invention.

[0028] 1. Acquisition of Thermophylla NK1-22

[0029] The algae strain (Thermoleptolyngbya sp. NK1-22) of the present invention is isolated from a biological mat sample at the mouth of a hot spring in Yunnan. The cell morphology was observed by light microscope and scanning electron microscope. figure 1 As shown, multiple cells strung together into a single row of cell filaments, blue-green in color, without nuclei, the filaments are of equal width up and down, and there are obvious colloidal sheaths outside, which is a typical shape of Thermophyllum spp. Genomic DNA was extracted and 16S rRNA gene sequence was amplified, as shown in SEQ ID NO: 1, compared with the 16S rRNA gene of microalgae in the database and a phylogenetic tree was constructed,...

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Abstract

The invention relates to Thermoleptolyngbya sp. NK1-22 with high carbohydrate yield. The Thermoleptolyngbya sp. is preserved in China General Microbiological Culture Collection Center on December 27,2019, and the preservation number is CGMCC 19155. The invention also relates to an application of the Thermoleptolyngbya sp. in carbohydrate production, and also relates to a method for culturing theThermoleptolyngbya sp. and a culture obtained by the method. The Thermoleptolyngbya sp. provided by the invention has 57% of soluble sugar content, and can secrete soluble sugar with higher concentration into a culture environment. Although the algal strain is separated from a thermal spring environment, but the strain has a very wide temperature tolerance range, grows well in a conventional environment of 30 DEG C and a high-temperature environment of 50 DEG C, has high carbohydrate yield, has high adaptability to high light intensity, provides high-temperature high light intensity during culture, can keep high-speed growth, and can reduce the possibility of contaminating miscellaneous algae and miscellaneous bacteria.

Description

technical field [0001] The invention relates to the field of applied microalgae, and more particularly relates to a hot spring cyanobacterium with high temperature tolerance and high carbohydrate yield and its application. Background technique [0002] Microalgae biotechnology can get rid of the predicament of "competing with people for food" and "competing with food for land" in traditional green chemical industry, and it is a photosynthetic biosynthesis route that has attracted much attention. However, with the rapid development of microalgae large-scale cultivation technology, the bottleneck problems that have not been discovered or estimated in laboratories or Chinese scales such as microorganisms, viruses, and protozoa contamination have become increasingly prominent. Breeding high-quality algae species, discovering new cultivation techniques, and developing high-quality products that meet the needs of the national economy and the people's livelihood are urgent problems...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P19/02C12P19/12C12R1/01
CPCC12N1/20C12P19/02C12P19/12C12R2001/01C12N1/205
Inventor 吕雪峰朱涛尼丁·克什李李辉谢玉曼齐凤霞
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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