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Microbial preparation containing multiple lactic acid bacteria and application of microbial preparation in corn silage

A technology of microbial preparation and lactic acid bacteria, applied in the field of microorganisms and silage fermentation, can solve the problems of less lactic acid production and less application, etc.

Active Publication Date: 2020-11-27
黑龙江省农业科学院畜牧兽医分院 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to the current research, the fermentation process of silage mainly depends on a variety of lactic acid bacteria, especially homofermentative lactic acid bacteria such as Lactobacillus plantarum, heterofermentative lactic acid bacteria such as Lactobacillus Brucella, Bifidobacterium bifidum, Lactobacillus brevis, etc. Less and undesired acetic acid product, less application

Method used

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  • Microbial preparation containing multiple lactic acid bacteria and application of microbial preparation in corn silage
  • Microbial preparation containing multiple lactic acid bacteria and application of microbial preparation in corn silage
  • Microbial preparation containing multiple lactic acid bacteria and application of microbial preparation in corn silage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The effect of embodiment 1 strain combination on corn silage

[0042] The experiments of Example 1 and Example 2 were carried out in multiple batches in the same season using the same raw material, and a variety of screened formulas were not shown due to lack of representativeness.

[0043] Additive 1: 5 parts by weight of bacteria powder, 90 parts by weight of glucose: the contents of Lactobacillus plantarum and Bifidobacterium bifidum are respectively 5×10 9 cfu / g and 1×10 9 cfu / g.

[0044] Additive 2: 5 parts by weight of bacteria powder, 90 parts by weight of glucose: wherein the contents of Lactobacillus plantarum and Bifidobacterium bifidum are respectively 5×10 9 cfu / g and 3×10 9 cfu / g.

[0045] Additive 3: 5 parts by weight of bacteria powder, 90 parts by weight of glucose: including 7×10 Lactobacillus plantarum 9 cfu / g.

[0046] Sensory evaluation:

[0047] All three groups of additives can make the silage reach the excellent level.

[0048] pH:

[004...

Embodiment 2

[0056] Example 2 Effect of Adding Lactic Acid / Lactate and Sodium Bicarbonate on Corn Silage

[0057] Additive 4: 5 parts by weight of bacteria powder, 90 parts by weight of glucose: the contents of Lactobacillus plantarum and Bifidobacterium bifidum are respectively 5×10 9 cfu / g and 1×10 9 cfu / g; another 15 parts by weight of lactic acid.

[0058] Additive 5: 5 parts by weight of bacteria powder, 90 parts by weight of glucose: the contents of Lactobacillus plantarum and Bifidobacterium bifidum are respectively 5×10 9 cfu / g and 1×10 9 cfu / g; another 15 parts by weight of sodium lactate.

[0059] Additive 6: 5 parts by weight of bacteria powder, 80 parts by weight of glucose, 15 parts by weight of sodium bicarbonate: wherein the contents of Lactobacillus plantarum and Bifidobacterium bifidum are respectively 5×10 9 cfu / g and 1×10 9 cfu / g; another 15 parts by weight of sodium lactate.

[0060]Additive 7: 5 parts by weight of bacteria powder, 80 parts by weight of glucose, 2...

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Abstract

The invention provides a microbial preparation containing multiple lactic acid bacteria and application of the microbial preparation in corn silage. The microbial preparation contains Lactobacillus plantarum, Bifidobacterium powder and auxiliary ingredients. The microbial preparation provides good mold inhibition performance and preservation performance through combination of homofermentative lactic acid bacteria and heterofermentative lactic acid bacteria and the addition of sodium lactate.

Description

technical field [0001] The invention belongs to the field of microorganisms and silage fermentation. Specifically, the invention provides a microbial preparation containing a variety of lactic acid bacteria and its application in corn silage, which contains Lactobacillus plantarum and Bifidobacterium bifidum powder and auxiliary ingredients . Background technique [0002] Silage refers to the conversion of carbohydrates into organic acids mainly lactic acid through anaerobic fermentation of lactic acid bacteria attached to silage raw materials under sealed conditions, so that the pH drops and the growth of harmful bacteria is inhibited, so that the feed can be preserved and optimized for a long time process of its nutritional value. Since ancient times, silage has been one of the main sources of animal husbandry feed in cold regions. The feed that can be silaged mainly includes corn, alfalfa, barley, oats, sorghum, milk vetch, sugar beets, potatoes, etc. Corn is the main fe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K30/18C12R1/25C12R1/01
CPCC12N1/20A23K30/18A23V2400/169A23V2400/517
Inventor 李旭业董扬尤海洋郭文凯李莉王敏杨淑萍王佳张超王洪宝刘丽秋刘秀玲郭春晖
Owner 黑龙江省农业科学院畜牧兽医分院
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