Method for increasing alkaloid content of radix sophorae tonkinensis tissue culture seedlings by utilizing selenium

Abstract

The invention discloses a method for increasing alkaloid content of radix sophorae tonkinensis tissue culture seedlings by utilizing selenium. The radix sophorae tonkinensis tissue culture seedlings are placed in a culture medium containing selenium for culture. The selenium element is added into the culture medium, so that the alkaloid content of radix sophorae tonkinensis is effectively increased, meanwhile the selenium absorption rate of plants is increased, and growth of radix sophorae tonkinensis tissue culture seedlings and accumulation of enzymes, hormones and main active ingredients inbodies can be adjusted.

Description

technical field [0001] The present invention relates to the field of biotechnology. More specifically, the present invention relates to a method for increasing the alkaloid content of the root tissue cultured seedlings of bean root by using selenium. Background technique [0002] Shandougen is a famous authentic medicinal material and a commonly used bulk traditional Chinese medicine in Guangxi, also known as Guangdougen, which is the dried root and rhizome of Sophora tonkinensis Gagnep. Bitter in the mouth, cold. Return lung, stomach warp. It has the effects of clearing away heat and detoxification, reducing swelling and relieving throat, and is used for stagnation of fire and poison, sore throat, sore throat, swollen gums, and aphtha of the mouth. It is the main raw material of nearly 30 kinds of Chinese patent medicine products for the treatment of hepatitis, sore throat, anti-tumor, hemorrhoids, acne, skin diseases, etc. It is used as decoction pieces in many prescrip...

AI Technical Summary

Problems solved by technology

In the prior art, the introduction of alkaloid precursors (amino acids) is often used to increase the alkaloid content inside the plant. The cell and tissue structure of the plant itself is diverse, so that the externally introduced alkaloid precursors (amino acids) may produce other by-products Synthetic reaction, and the plant has a poor absorption rate of externally synthesized alkaloids, which eventually leads to poor regulation of alkaloid content;

[0005] Adding a certain amount of selenium can effectively increase the content of matrine and oxymatrine, but if the concentration of selenium is too high, the absorption and utilization of selenium will decrease, especially if the concentration of inorganic selenium is too high, it will increase the toxicity of plant cells, thereby inhibiting the The growth and development of plants; how to achieve the double superior effect of improving the growth and development of plants and increasing the content of alkaloids in plants by adding selenium is a technical difficulty to be broken through in this field

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