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Mounting preparation method for observing vein network structure of turfgrass

A technology of network structure and turfgrass, which is applied in the field of film preparation for observation of turfgrass vein network structure, can solve problems such as the method for measuring the turfgrass vein network structure that is not disclosed, and achieve adaptability, avoid damage, and universal adaptability Effect

Active Publication Date: 2020-12-01
TIANJIN AGRICULTURE COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the prior art, the research on the vein network structure mostly focuses on general broad-leaved plants and large monocotyledonous plants, as well as vein bookmarks and vein flowers, etc. There is no disclosed method for measuring the vein network structure of turfgrass

Method used

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  • Mounting preparation method for observing vein network structure of turfgrass
  • Mounting preparation method for observing vein network structure of turfgrass
  • Mounting preparation method for observing vein network structure of turfgrass

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preparation example Construction

[0044] A method for preparing slices for observing the vein network structure of turfgrass, specifically comprising the following method steps:

[0045] ⑴ Leaf collection and pretreatment

[0046]Select turfgrass leaves to be treated, soak them in distilled water for 10 minutes, take out the leaves, and let them stand on the filter paper for 10-20 seconds to remove the water and impurities on the surface of the leaves, and use transparent tape to remove the fluff on the front and back of the leaves. The length of treatment is 6 ~8cm, then apply colorless nail polish evenly on the front and back of the leaves, after it dries, remove the nail polish with scotch tape, and cut leaves 4-6cm long from the treated part;

[0047] ⑵Decolorization treatment

[0048] Soak the treated grass blades in 5% NaOH solution for 2 to 3 days, and change the soaking solution once in the middle;

[0049] ⑶ Vein dyeing and color separation

[0050] According to the type of lawn grass leaves, two m...

Embodiment 1

[0064] The present embodiment relates to a method for preparing slices for observing the vein network structure of bluegrass (Poapratensis), comprising the following steps:

[0065] 1) Leaf collection and pretreatment:

[0066] Select freshly mature bluegrass leaves and soak them in distilled water for 10 minutes; take out the leaves, use filter paper or paper-free toilet paper to deal with the water and impurities on the surface of the leaves, and then place them on the filter paper for 10-20 seconds; Stick the hair on the front and back of the middle part of the leaves, and the length of the treatment is 6-8cm; then apply the nail polish evenly on the front and back of the leaves, and after it dries, remove the nail polish with scotch tape. 4-6 cm long bluegrass leaves were cut from the treated parts as experimental materials.

[0067] 2) Decolorization of leaves: soak the treated bluegrass leaves in 5% NaOH solution for 2 days, and change the soaking solution once in the m...

Embodiment 2

[0072] This embodiment relates to a method for preparing slices for observing the vein network structure of creeping bentgrass (Agrostis stolonifera), the steps of which are the same as those in Embodiment 1. The only difference is that because the leaves of creeping bentgrass are smaller, the whole leaves are used as experimental materials in the leaf pretreatment stage, and the rehydration time is adjusted to 2h. The fabricated mounts were photographed under a LEICADM4000B LED optical microscope, and the leaf vein images were measured with LAS AF Lite image analysis software. Leaf vein images of creeping bentgrass such as figure 2 As shown, the transverse vein density is 0.904mm / mm 2 ;Large longitudinal vein density is 1.897mm / mm 2 ; The density of small longitudinal veins is 2.923mm / mm 2 . The ratios of transverse veins, large longitudinal veins and small longitudinal veins to the total leaf veins were 15.8%, 33.14% and 51.06%, respectively.

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Abstract

The invention relates to a mounting preparation method for observing a vein network structure of turfgrass. The mounting preparation method comprises the following steps: (1) leave collection and pre-treating; (2) decoloring: soaking leaves with 5% NaOH for 2-3 days; (3) vein dyeing and color separation: selecting two modes according to the variety of turfgrass to carry out sharpening treatment onveins: a, carrying out crocus sativus dyeing and color separation dehydration; b, carrying out crocus sativus-solid green dyeing and color separation dehydration; and (4) rehydration: soaking the leaves in water for 2-24 hours (a) or 20-48 hours (b), viewing and shooting images, and, finally, analyzing the vein density and percentage of each level by using software. The mounting preparation method for observing the vein network structure of the turfgrass is improved from multiple steps of early-stage treatment, dyeing, color separation, dehydration and the like of the leaves, and is easy andconvenient to operate, safe, environment-friendly, short in preparation period, good in flaking effect and clear in image. The method has universal adaptability to the turfgrass, can quickly obtain aclear vein image, and is used for the related research of the turfgrass vein network structure.

Description

technical field [0001] The invention belongs to the field of ecology and environmental protection, and relates to the analysis of the vein network structure of turfgrass, in particular to a method for preparing slices for observing the vein network structure of turfgrass. Background technique [0002] Turfgrass has a well-developed root system and high ground coverage. A large number of studies have shown that it plays a significant role in sewage purification and heavy metal absorption, and the lawn has both ornamental and sports performances. Therefore, it is in great demand in urban construction and in the construction of ecological civilization. Today, it is of great ecological significance to screen more excellent turfgrass varieties. In order to reduce evapotranspiration, turfgrass leaves mostly have the characteristics of low water content, rough texture, and waxy or hairy covering. Different turfgrass species have different leaf phenotype characteristics and water c...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N1/30G01N21/84
CPCG01N1/28G01N1/30G01N21/84G01N2021/8466
Inventor 孙小玲宋重升柴旭旭宋午椰崔朝伟
Owner TIANJIN AGRICULTURE COLLEGE
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