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The use of the buche gene snp site and the detection kit for the susceptibility analysis and detection kit of hypertgemia in the population exposed to neonicotinoid pesticides

A detection kit and neonicotinoid technology, applied in the determination/testing of microorganisms, recombinant DNA technology, DNA/RNA fragments, etc., can solve problems such as interference with the normal metabolic function of the body, organic and functional damage to the liver, etc.

Active Publication Date: 2021-06-08
BEIJING MUNICIPAL INST OF LABOUR PROTECTION +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Neonicotinoid pesticides not only cause organic and functional damage to the liver, but also have the potential risk of interfering with the normal metabolic function of the body

Method used

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  • The use of the buche gene snp site and the detection kit for the susceptibility analysis and detection kit of hypertgemia in the population exposed to neonicotinoid pesticides
  • The use of the buche gene snp site and the detection kit for the susceptibility analysis and detection kit of hypertgemia in the population exposed to neonicotinoid pesticides
  • The use of the buche gene snp site and the detection kit for the susceptibility analysis and detection kit of hypertgemia in the population exposed to neonicotinoid pesticides

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Materials and Methods

[0043] Research object

[0044] This experiment was included in 72 new nicotine pesticide contacts and 78 non-nicotine pesticides in contact with the controller. All research objects signed an informed consent, complete the corresponding epidemiological survey, and provide 1-3ml peripheral blood specimens.

[0045] 2. Extraction and testing of peripheral blood genome DNA

[0046] Genomic DNA in peripheral blood was extracted with genomic DNA column small amount extraction kit (Kang Yu Century Biotechnology Co., Ltd.). Subsequently, DNA sample quality inspection was performed using a NanoDrop micro-nucleic acid concentration meter (Fisher) to perform DNA sample quality inspection with agarose gel electrophoresis.

[0047] 3.PCR reaction

[0048] 3.1 PCR primer design and synthesis

[0049] PRIMER3 primer design software ( https: / / primer3.ut.ee / ) Design of RS1803274-bit PCR amplification primer. Where: Primer Size: 18-25; Product Size Ranges: 100-300; ...

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Abstract

The application discloses the use of a BuChE gene SNP site and a detection kit for the susceptibility analysis and detection of hyperTGemia in populations exposed to neonicotinoid pesticides; Detection primers. The present application discovered the characteristics of the BuChE gene rs1803274 mutation site in the population exposed to neonicotinoid pesticides and its association with the risk of hyperTGemia. And thus provide a detection kit and primers for the susceptibility analysis of hyperTGemia in the population exposed to neonicotinoid pesticides.

Description

Technical field [0001] The present invention relates to the field of molecular labeling, and in particular to the application of the Buche gene SNP site in the preparation of the high Tg hematomosis of the new nicotine pesticide contact crowd. Background technique [0002] The new nicotine pesticide application has a wide range of 5.3% of the global pesticide market, and the compound annual growth rate of 4.1% to 9.6% in 2014 and 2019, causing a pesticide residue that cannot be ignored. New nicotine pesticides can be roughly divided into three categories of chemical structures, namely N-nitrin, nitro methylene, N-cyanohydridine. Among them, N-nitridin's pyrlin is the largest in my country's agricultural sectors, and the content of the skin and respiratory tract into the body accounts for about 0.21%, and the exposure level can reach 14.71 μg / g creatinine. Health hazards need to be emphasized. [0003] New nicotine pesticides do not only cause liver quality and functional damage...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883C12Q1/6806C12N15/11
CPCC12Q1/6806C12Q1/6883C12Q2600/156C12Q2531/113C12Q2521/319C12Q2521/501C12Q2563/173
Inventor 周兴藩余珉唐仕川王煜倩
Owner BEIJING MUNICIPAL INST OF LABOUR PROTECTION