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A kind of microbial complex flora for biomineralization and its preparation and application

A technology of microbial composite bacteria and biomineralization, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of low consolidation rate of consolidated loose particles and low strength of consolidated bodies, and achieve cementation The effect is good, the strength of the consolidated body is improved, and the effect of increasing the speed

Active Publication Date: 2022-01-07
HUAZHONG UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at technical defects or improvement needs such as low consolidation rate during biomineralization and low strength of the consolidated body in the prior art, the purpose of the present invention is to provide a microbial composite flora for biomineralization and its preparation and application , wherein the microbial composite flora is obtained by selecting specific first microorganisms and second microorganisms, the microbial composite flora has high urease activity and high carbonic anhydrase activity, and the composite flora liquid obtained by compounding is especially useful for biomineralization, Effectively solve the defects and deficiencies in the prior art that the consolidation rate of loose particles consolidated by natural strains of biocement is low, and the strength of the obtained consolidated body is not high; the microbial composite flora in the present invention is used for biological consolidation, which can improve the The speed of loose particles is also significantly improved.

Method used

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  • A kind of microbial complex flora for biomineralization and its preparation and application
  • A kind of microbial complex flora for biomineralization and its preparation and application
  • A kind of microbial complex flora for biomineralization and its preparation and application

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example 1

[0036] Weigh tryptone 12.0g, yeast extract 4.0g, (NH 4 ) 2 SO 4 3.0g, KH 2 PO 4 7.0g, Na 2 HPO 4 7.3g, glucose 1.0g, lactose 3.0g, MgSO 4 0.1g, 3ml glycerin, dissolved in purified water, dilute to 1L, measure pH 7.5, evenly distribute into ten 500ml Erlenmeyer flasks, sterilize at 118°C for 20min, take out and cool to room temperature for later use. Take a bottle of the above-mentioned culture medium, and add in turn chloramphenicol sterilized by suction to a final concentration of 20.0mg / L and NiCl 2 To a final concentration of 5.0 mg / L, the strain of microorganism (A) with high urease activity was inserted, and cultured with shaking at 200 rpm at 37°C for 30 hours to obtain the bacterial liquid of microorganism (A) with high urease activity. Take another bottle of the above-mentioned sterilized culture medium, and add ampicillin that was sterilized by suction to a final concentration of 50.0mg / L and ZnCl 2 To a final concentration of 5.0mg / L, insert microorganisms...

example 2

[0039] Weigh tryptone 10.0g, yeast extract 5.0g, (NH 4 ) 2 SO 4 3.5g, KH 2 PO 4 6.8g, Na 2 HPO 4 7.1g, glucose 0.5g, lactose 2.0g, MgSO 4 0.2g, 5ml glycerin, add purified water to dissolve, dilute to 1L, evenly distribute into ten 500ml Erlenmeyer flasks, sterilize at 118°C for 20min, take out and cool to room temperature for use. Take a bottle of the above-mentioned culture medium, add in turn chloramphenicol sterilized by suction to a final concentration of 5.0mg / L and NiCl 2 To a final concentration of 10.0 mg / L, the microorganism (A) strain with high urease activity was inserted, and cultured with shaking at 200 rpm at 37° C. for 28 hours to obtain the bacterial liquid of microorganism (A) with high urease activity. Take another bottle of sterilized culture medium, add ampicillin sterilized by suction to a final concentration of 100.0mg / L and ZnCl 2 To a final concentration of 10.0 mg / L, insert microorganisms (B) strains with high carbonic anhydrase activity, sh...

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Abstract

The invention belongs to the field of biomineralization applications, and discloses a microbial composite flora for biomineralization and its preparation and application. The microbial composite flora for biomineralization includes a first microorganism with high urease activity and The second microorganism with high carbonic anhydrase activity, the first microorganism and the second microorganism are all preserved in China Center for Type Culture Collection, and the preservation numbers are CCTCC M 2020469 and CCTCC M 2020468 respectively. The present invention obtains a microbial composite flora by selecting specific first microorganisms and second microorganisms. The microbial composite flora has high urease activity and high carbonic anhydrase activity, and the composite flora liquid obtained by compounding is especially useful for biomineralization. The invention effectively solves the defects and deficiencies in the prior art, such as low consolidation rate of loose particles consolidated by natural strains of biocement, and low strength of the obtained consolidated body.

Description

technical field [0001] The invention belongs to the application field of biomineralization, and more specifically relates to a microbial composite flora for biomineralization and its preparation and application. The microbial composite flora is an enhanced bio-consolidation composite flora, especially having Excellent ability to consolidate loose particles. Background technique [0002] Calcium carbonate precipitation induced by microorganisms is a kind of bioconsolidation phenomenon that is ubiquitous in nature. Some microorganisms can form calcite crystals outside the cells through their own metabolism and the enzymatic action between the surrounding environment medium, so that the loose sand Cementation and mineralization of soil particles. At present, calcium carbonate precipitation induced by microorganisms has been widely used in research fields such as building foundation reinforcement, concrete crack repair, and water conservancy engineering anti-seepage due to its ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C09K17/06C09K17/14C09K103/00C12R1/125C12R1/19
CPCC12N1/20C09K17/06C09K17/14C12R2001/125C12N1/205C12R2001/19
Inventor 闫云君王磊龚梦瑶王绪霞许赟姚敦璠徐莉张后今阎金勇
Owner HUAZHONG UNIV OF SCI & TECH
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