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Rapid and ultra-sensitive ATP detection method and application thereof

A detection method and ultra-sensitive technology, applied in the fields of diagnosis and biological cell molecule detection, can solve the problems of less ATP distribution, large background interference and rapid loss, and achieve the effect of less time-consuming, simple operation and ultra-sensitive detection.

Inactive Publication Date: 2021-02-05
XUCHANG UNIV
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  • Abstract
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  • Claims
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Problems solved by technology

However, most of the above methods are based on the average value of the overall events of the target molecule, and the background interference is relatively large, and ATP has less extracellular distribution, low concentration, and rapid loss, making it difficult to detect

Method used

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  • Rapid and ultra-sensitive ATP detection method and application thereof
  • Rapid and ultra-sensitive ATP detection method and application thereof
  • Rapid and ultra-sensitive ATP detection method and application thereof

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Embodiment

[0027] The present invention provides a kind of fast, supersensitive ATP detection method and application thereof, and described ATP detection method comprises three basic steps, is respectively:

[0028] Step 1: Preparation of tapered polymer nanochannels; exposing a PET film with a thickness of 8-20 microns containing a single ion bombardment track to ultraviolet light with a wavelength of 365nm overnight, so that the original particles on the PET film The small track of the irradiated PET film is pasted on two polytetrafluoroethylene semi-electrolytic cells, so that the electrolytic cells form two mutually isolated cavities, and 9M NaOH is added to one side of the cavity as an etching method. 1M HCOOH and 1M KCl were added to the other side as the etching stop solution, and Pt electrodes were respectively inserted into the solutions on both sides, the Pt electrode on one side of the etching solution was connected to the positive electrode of the power supply, The Pt electro...

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Abstract

The invention provides a rapid and ultra-sensitive ATP detection method and application thereof, and the ATP detection method comprises three basic steps: preparation of a conical polymer nano-channel; ATP detection; and data processing. The detection method has the characteristics of simple operation, less time consumption and low cost when used for detecting ATP molecules of biological cells; the method realizes specific detection of the ATP molecules by designing DNA sequences of capture probes DNA (H1 and H2) and auxiliary probes DNA (H3 and H4), has good anti-interference ability, does not need molecular markers when used for detecting the ATP molecules of the biological cells, and can realize high-flux and ultra-sensitive detection. The detection limit can reach the pM level, and meanwhile, single-molecule fingerprint information can be observed.

Description

technical field [0001] The invention belongs to the technical field of biological cell molecular detection and diagnosis, and in particular relates to a fast and ultrasensitive ATP detection method and its application. Background technique [0002] Adenosine triphosphate (ATP) is the main provider of intracellular energy source, it participates in intracellular metabolic process, and plays an important role in maintaining the normal function of organisms. In recent years, various diseases have been confirmed to be related to abnormal ATP content, such as cardiovascular disease, Parkinson's disease and Alzheimer's disease. And because the process of cell proliferation, differentiation and death will cause the change of ATP content, so by monitoring the change of ATP content, it can be used to evaluate drug sensitivity and cellular immune activity, and then promote the research of cell physiology and pathology, cancer regulation mechanism, etc. . In addition, ATP is also a c...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/30G01N27/327
CPCG01N27/26G01N27/30G01N27/3278
Inventor 孙红姚川许志红张时星杨玲李会张羽翔
Owner XUCHANG UNIV
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