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Glycyrrhizin-glycol chitosan conjugate-coated iron oxide nanoparticles and use thereof

A technology of glycyrrhizin and nanoparticles, applied in nanotechnology, nanotechnology, nanomedicine, etc., can solve the problems of islet cell transplantation and other problems

Active Publication Date: 2021-02-12
IUCF HYU (IND UNIV COOP FOUND HANYANG UNIV)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Restricted by organ donors, this is also a factor that makes the islet cell transplantation itself difficult

Method used

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  • Glycyrrhizin-glycol chitosan conjugate-coated iron oxide nanoparticles and use thereof
  • Glycyrrhizin-glycol chitosan conjugate-coated iron oxide nanoparticles and use thereof
  • Glycyrrhizin-glycol chitosan conjugate-coated iron oxide nanoparticles and use thereof

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0082] Example 1: Preparation of Glycyrrhizin-Ethylene Glycol Chitosan Coated Iron Oxide Nanoparticles

[0083] 1-1. Synthesis of glycyrrhizin-ethylene glycol chitosan

[0084] pass figure 1 Glycyrrhizin-ethylene glycol chitosan (GC) was prepared by the synthetic method disclosed in . 411.47mg glycyrrhizin ammonium salt (Sigma Aldrich (Sigma Aldrich), the United States) and 205.74mg ethylene glycol chitosan (Wako Pure Chemical Industry Co., Ltd. (WAKO PURE CHEMICAL INDUSTRIES), Japan) respectively at 4 ℃ Added to 10 ml and 20 ml of carbonate buffer pH 9.5 and dissolved, and 214 mg of sodium periodate (Sigma Aldrich) was dissolved in 20 ml of triple distilled water (DW). When the sodium periodate was completely dissolved, the resulting solution was added to 10 ml of the glycyrrhizin solution, and the resulting mixture was allowed to react at 4° C. for 90 minutes under light-shielding conditions. When the ethylene glycol chitosan was completely dissolved, the resulting soluti...

example 2

[0094] Example 2: Confirmation of GC-SPIO Uptake by Islet Cells

[0095] 2-1. Isolation of pancreatic islet cells (islets)

[0096] Collagenase P was dissolved in Hanks' balanced salt solution (HBSS) at a concentration of 1 mg / kg, and the resulting solution was intraductally injected into male SD rats. Afterwards, the pancreas was isolated and stored in water at 37°C for 15 minutes, and the isolated islet cells were washed with Medium 199. Washed islet cells were purified and further purified by centrifugation with Histopaque (Sigma, USA). The purified islet cells were cultured in RPMI-1640 (Invitrogen, USA) containing 10% fetal bovine serum and 1% antibiotics for 24 hours.

[0097] 2-2. Confirmation of GC-SPIO uptake by islet cells

[0098] Although GC-SPIO can be absorbed into islet cells through endocytosis, GC-SPIO has the disadvantage of low uptake efficiency and random uptake. Therefore, a new method for efficient uptake of GC-SPIO in islet cells was devised. The four...

example 3

[0107] Example 3: Confirmation of Optimum Conditions for a Magnetic Open / Close System

[0108] 3-1. Optimizing GC-SPIO treatment concentration

[0109] After culturing the islet cells (200 IEQ) in 96-well plates, the islet cells were treated with various concentrations of GC-SPIO (0, 2.5, 5, 10, 20 and 45 μg / ml) by the on / off system method. Specifically, a total of 12 times (1 cycle × 12) were used to treat 35π Petri dishes including islet cells and 3ml RPMI (PBS 10%, PS 1%) medium at corresponding concentrations with GC-SPIO, and apply Magnetic force was applied for 1 minute, pipetting was performed for 1 minute, and the islet cells were incubated for 1 minute without any magnetic treatment. Afterwards, the GC-SPIO not taken up in the islet cells was separated from the islet cells by a cell sieve. Next, according to the method of Example 2-2, the iron absorbance analysis kit was used for analysis.

[0110] Therefore, if Figure 7 As shown, it can be seen that the amount t...

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Abstract

The present invention relates to glycyrrhizin-glycol chitosan conjugate-coated nanoparticles, islet cells, prepared using same, for transplantation, and an MRI imaging composition comprising same. Iftransplanted, the islet cells comprising the nanoparticles can suppress a post-transplantation immune response. The present invention can provide islet cells for transplantation that can be transplanted to a certain region by magnetic force induction and can be tracked by MRI.

Description

technical field [0001] The present invention relates to glycyrrhizin-ethylene glycol chitosan conjugate-coated nanoparticles and islet cell composition for transplantation using the nanoparticles. Background technique [0002] Type 1 diabetes results from a decrease in the number of normally functioning beta cells in the pancreas, resulting in insufficient insulin secretion. Therefore, blood sugar is not regulated normally, and hyperglycemia occurs, which causes various complications. [0003] As a method for treating this type 1 diabetes, the most widely used so far is to use an insulin syringe to artificially inject insulin to temporarily regulate blood sugar. In order to overcome the temporary blood sugar regulation problem of insulin injection, the transplantation method of heterogeneous islet cells has been extensively studied. Although most cells have been clinically transplanted into the liver site, the disadvantage is that the cells are injected through the portal ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/51A61K9/50A61K49/18A61P3/10A61L27/36
CPCA61L27/36A61P3/10A61K49/1863A61K49/1833A61K47/6929A61K47/6923A61K9/5115B82Y5/00A61K9/5123A61K9/5161A61K9/5094A61L27/3683A61L2430/40A61L2400/12A61K47/554A61K35/39
Inventor 李东玧张秀彬李相俊
Owner IUCF HYU (IND UNIV COOP FOUND HANYANG UNIV)
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