Glycyrrhizin-glycol chitosan conjugate-coated iron oxide nanoparticles and use thereof
A technology of glycyrrhizin and nanoparticles, applied in nanotechnology, nanotechnology, nanomedicine, etc., can solve the problems of islet cell transplantation and other problems
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example 1
[0082] Example 1: Preparation of Glycyrrhizin-Ethylene Glycol Chitosan Coated Iron Oxide Nanoparticles
[0083] 1-1. Synthesis of glycyrrhizin-ethylene glycol chitosan
[0084] pass figure 1 Glycyrrhizin-ethylene glycol chitosan (GC) was prepared by the synthetic method disclosed in . 411.47mg glycyrrhizin ammonium salt (Sigma Aldrich (Sigma Aldrich), the United States) and 205.74mg ethylene glycol chitosan (Wako Pure Chemical Industry Co., Ltd. (WAKO PURE CHEMICAL INDUSTRIES), Japan) respectively at 4 ℃ Added to 10 ml and 20 ml of carbonate buffer pH 9.5 and dissolved, and 214 mg of sodium periodate (Sigma Aldrich) was dissolved in 20 ml of triple distilled water (DW). When the sodium periodate was completely dissolved, the resulting solution was added to 10 ml of the glycyrrhizin solution, and the resulting mixture was allowed to react at 4° C. for 90 minutes under light-shielding conditions. When the ethylene glycol chitosan was completely dissolved, the resulting soluti...
example 2
[0094] Example 2: Confirmation of GC-SPIO Uptake by Islet Cells
[0095] 2-1. Isolation of pancreatic islet cells (islets)
[0096] Collagenase P was dissolved in Hanks' balanced salt solution (HBSS) at a concentration of 1 mg / kg, and the resulting solution was intraductally injected into male SD rats. Afterwards, the pancreas was isolated and stored in water at 37°C for 15 minutes, and the isolated islet cells were washed with Medium 199. Washed islet cells were purified and further purified by centrifugation with Histopaque (Sigma, USA). The purified islet cells were cultured in RPMI-1640 (Invitrogen, USA) containing 10% fetal bovine serum and 1% antibiotics for 24 hours.
[0097] 2-2. Confirmation of GC-SPIO uptake by islet cells
[0098] Although GC-SPIO can be absorbed into islet cells through endocytosis, GC-SPIO has the disadvantage of low uptake efficiency and random uptake. Therefore, a new method for efficient uptake of GC-SPIO in islet cells was devised. The four...
example 3
[0107] Example 3: Confirmation of Optimum Conditions for a Magnetic Open / Close System
[0108] 3-1. Optimizing GC-SPIO treatment concentration
[0109] After culturing the islet cells (200 IEQ) in 96-well plates, the islet cells were treated with various concentrations of GC-SPIO (0, 2.5, 5, 10, 20 and 45 μg / ml) by the on / off system method. Specifically, a total of 12 times (1 cycle × 12) were used to treat 35π Petri dishes including islet cells and 3ml RPMI (PBS 10%, PS 1%) medium at corresponding concentrations with GC-SPIO, and apply Magnetic force was applied for 1 minute, pipetting was performed for 1 minute, and the islet cells were incubated for 1 minute without any magnetic treatment. Afterwards, the GC-SPIO not taken up in the islet cells was separated from the islet cells by a cell sieve. Next, according to the method of Example 2-2, the iron absorbance analysis kit was used for analysis.
[0110] Therefore, if Figure 7 As shown, it can be seen that the amount t...
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