Method for establishing lycoris longituba regeneration system through callus approach

The technology of a long tube lycoris and a way is applied in the field of tissue culture and reproduction of the long tube lycora, and can solve the problems of low proliferation rate, rapid propagation method of pollution rate culture, and high pollution rate.

Active Publication Date: 2021-02-23
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Previous studies have obtained the proliferation medium and rooting medium of other species of Amaryllis, most of which use bulbs as explants, and often have the problems of high pollution ...

Method used

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Embodiment 1

[0046] 1. Selection and disinfection of explants: At the end of August, the apex of the flower axis of the long-tube Lycoris at the early flowering stage was selected as the explants. The disinfection method was to shake with detergent solution for 15 minutes, rinse with running water for 30 minutes, and use it on the sterile operating table. Disinfect with 75% ethanol for 30 seconds, then disinfect with 0.2% mercuric chloride for 15 minutes, and finally wash with sterile water for 5 times, each time for 1 minute, and treat 100 explants in total;

[0047] 2. Induction of adventitious buds: After detoxification, the explants were cut with the apex of the floral axis as the center and kept 1cm long (the lengths of several branches are equal), and the explants were inoculated in the induction medium MS+6-BA 2mg / L +NAA0.2 mg / L for adventitious bud induction, control the culture temperature at 26°C,

[0048] First cultivate in the dark for 24 hours, then carry out red light cultur...

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Abstract

The invention provides a method for establishing a lycoris longituba regeneration system through a callus approach. The method comprises the following steps of taking healthy leaves of aseptic seedlings of lycoris longituba to be inoculated into an induction culture medium for callus induction, controlling the culture temperature at 24-26 DEG C, and culturing for 30 days under the conditions thatthe illumination time is 10-14 h/day and the illumination intensity is 1400-1800lx; then transferring obtained controlling calluses into a differential medium to be cultured for 30 days; and finally,inoculating aseptic seedlings of colored leaf poplar into a rooting medium to be subjected to rooting culture for 20 days. By means of the method, the problem of in-vitro rapid propagation of the lycoris longituba can be effectively solved.

Description

technical field [0001] The invention relates to a method for tissue culture and propagation of Lycoris longifolia, in particular to a method for establishing a regeneration system of Lycoris longifolia through a callus approach, and belongs to the technical field of seedling propagation of herbaceous ornamental plants. Background technique [0002] Long tube Lycoris ( Lycoris radiata (L'Her.) Herb.) is a kind of Amaryllidaceae Lycoris. Perennial herb. Underground stems are thick. Ligule-shaped, pumped from base after flowering. The scape is 30-60 cm high, the umbel is terminal, the flower is bright red, the flower tube is short, the perianth is narrowly oblanceolate, rolled outward, the stamens and styles protrude, the posture is beautiful, the flowering period is from July to September, Blooms in July and grows in summer. Sexually likes a damp environment, afraid of direct sunlight, and should grow in loose and fertile sandy loam. Native to China and Japan, it is wide...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 束晓春王忠张凤姣庄维兵王涛王宁
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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