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A kind of preparation method and application of Polygonum multiflorum extract
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A kind of technology of Shouwuteng and extract, which is applied in the field of preparation of Shouwuteng extract
Active Publication Date: 2022-02-08
CHINA TOBACCO YUNNAN IND
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Problems solved by technology
Existing studies mainly focus on the antibacterial screening of Shouwuteng extract or compounds against common food-borne pathogens, but there are no reports on Shouwuteng extracts for the prevention and treatment of oral pathogens
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preparation example Construction
[0028] The present disclosure provides a preparation method of Radix Polygoni Multiflori extract, comprising the following steps:
[0029] Step (1): pulverize Radix Polygoni Multiflori, soak in petroleum ether for a period of time, and then air-dry to obtain Radix Radix Radix Radix Powder.
[0030] In order to more efficiently extract the active ingredients in Shouwu Teng, in step (1), the Shouwu Teng is crushed to a particle size of 35 mesh to 45 mesh.
[0031] In order to more efficiently extract the active ingredients in Shouwu vine, the soaking time in step (1) is 15h-33h.
[0032] In order to more efficiently extract the active ingredients in Shouwu vine, the air-drying time in step (1) is greater than 6 hours.
[0033] Step (2): Ultrasonic extraction of Shouwu Teng powder with an organic solvent, and filtration to obtain Shouwu Teng extract, wherein the mass volume ratio of Shouwu Teng to the organic solvent is 1:(15-25), and Shouwu Teng's The unit of mass is g, and th...
Embodiment 1
[0046] (1) Weigh 5 g of Shouwu vine (purchased from Yunnan Fulintang Pharmaceutical Co., Ltd.), place it in a constant temperature drying oven, dry it to constant weight, pass through a 40-mesh sieve after crushing, and soak the small-sized powder in petroleum ether (boiling 60-90°C) for 24 hours, and then placed in a fume hood to air-dry for more than 6 hours to obtain Shouwu vine powder;
[0047] (2) According to the solid-liquid ratio of 1:25, use 50% volume fraction of ethanol (mL) to ultrasonically extract Shouwu vine powder, ultrasonic power is 450W, ultrasonic temperature is 40°C, ultrasonic time is 20min, and Shouwu is obtained by filtration Rattan extract;
[0048] (3) Shouwu vine extract was concentrated under reduced pressure at -15in.Hg and 80°C to a density of 1.200g / mL±0.005g / mL, and then freeze-dried at -35°C for 20h to obtain Shouwu Vine extract.
[0049] Determination of the size of the inhibition zone of Radix Polygoni Multiflori extract, the specific steps...
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Abstract
The invention discloses a preparation method and application of an extract of Shouwu vine. The preparation method comprises the following steps: pulverizing Shouwu vine, soaking in petroleum ether for a period of time and air-drying to obtain Shouwu vine powder; using an organic solvent to treat Shouwu vine The powder is ultrasonically extracted, and filtered to obtain the Shouwu vine extract, wherein the mass volume ratio of Shouwu vine to the organic solvent is 1:(15-25), the unit of the quality of Shouwu vine is g, and the unit of the volume of the organic solvent is It is mL; the Shouwu Teng extract is concentrated under reduced pressure to obtain the Shouwu Teng concentrated solution; the Shouwu Teng concentrated solution is subjected to freeze-drying treatment to obtain the Shouwu Teng extract. The preparation method of the Radix Polygonum extract of the present disclosure can quickly and efficiently obtain the Radix Polygonum extract with high purity, and the obtained Radix Radix Radix Extract can be used to inhibit Fusobacterium nucleatum.
Description
technical field [0001] The present invention relates to the field of natural extracts, more specifically, to a preparation method and application of Radix Polygoni Multiflori extract. Background technique [0002] The oral cavity is colonized with a large number and rich variety of microbial communities. The structural characteristics and stability of these microbial communities are closely related to the oral and general health of the host. Their pathological changes can induce dental caries, periodontal disease and other oral infectious diseases , It is also related to systemic diseases such as diabetes and rheumatoid arthritis. The oral microbial community is changing dynamically due to the semi-open ecological environment of the oral cavity. Studies have shown that certain oral microorganisms can induce oral and other systemic diseases of the host, for example, Streptococcus mutans is the main pathogenic bacteria of dental caries, Porphyromonas gingivalis is the main ca...
Claims
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