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Application of acetyltransferase osg2 gene and its encoded protein

A technology of acetyltransferase and gene encoding, applied in the field of genetic engineering

Active Publication Date: 2022-07-15
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are currently 7 kinds of N-terminal acetyltransferases that have been discovered, but the research is mainly concentrated on humans, and there are few studies on the function of N-terminal acetyltransferase genes in plants, especially the role of acetyltransferase in plant stress resistance. Aspects of research

Method used

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  • Application of acetyltransferase osg2 gene and its encoded protein
  • Application of acetyltransferase osg2 gene and its encoded protein
  • Application of acetyltransferase osg2 gene and its encoded protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: Acquisition of transgenic rice material

[0034] 1. Obtainment of transgenic mutant plants

[0035] The plant materials used in this study include wild-type Nipponbare rice and two mutant plants, and the two mutant plants are named osg2-1 and osg2-2 respectively. After gene editing by CRISPRCas9 technology, the result of gene editing is to produce two allelic mutants with different mutation sites. The editing method is as follows: figure 1 shown.

[0036] The full length of OsG2 gene is 4256bp, with figure 1 Gene structure diagram shown. Among them, NIP represents wild-type Nipponbare rice, which is used as a control; in order to construct mutant plants of OsG2 gene, CRISPR-Cas9 gene editing technology was used to create them. The main methods are as follows:

[0037] The transformed plants of the T0 generation of rice were detected by PCR sequencing, and the double-stranded homozygous edited mutants were screened and named osg2-1 and osg2-2, respectively....

Embodiment 2

[0058] Embodiment 2: the response situation of analysis OsG2 of salt stress treatment

[0059] First, the wild-type Nipponbare was used for salt stress treatment, and the response of OsG2 was analyzed. The specific process is as follows: select wild-type Nipponbare rice seeds in good development condition, put them into a petri dish containing a small amount of sterile water without shelling, and lay a layer of absorbent paper on the bottom of the petri dish. Put the petri dish containing the seeds in a 30-35 ℃ incubator for two days, and then transfer the seeds to an incubator with a temperature of about 25 ℃ after the seeds are white and broken to achieve the purpose of promoting germination and rooting. It should be noted that in the process of seed soaking and germination, it is necessary to diligently replace the sterile water in the petri dish to ensure the normal respiration and growth of the seeds.

[0060] The rice seedlings with the same growth status after germinat...

Embodiment 3

[0068] Example 3: Detection of salt sensitivity of transgenic mutant plants

[0069] Experimental materials: wild-type Nipponbare, OsG2 gene allelic mutant

[0070] Treatment method: The germination method of rice seeds is as described in Example 2. The wild-type Nipponbare and mutant seedlings with the same growth state were selected, transferred to Hoagland nutrient solution containing 150 mM NaCl, and treated for 0 days, 5 days and 7 days respectively. The morphological changes of leaves in the control group and the experimental group were recorded by phenotype. like image 3 shown, the mutant plants were more sensitive to salt stress than the wild-type Nipponbare.

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Abstract

The invention discloses the application of an acetyltransferase OsG2 gene and the encoded protein thereof. The amino acid sequence of the protein is shown in SEQ ID No: 2. The invention improves the salt stress resistance of crops such as rice by overexpressing the OsG2 gene. The invention provides an important way for cultivating rice with enhanced salt stress resistance, and can be applied to cultivate rice with enhanced salt stress resistance in agricultural production. , for energy saving and water saving, utilization of saline-alkali land, increase of grain output, etc.

Description

technical field [0001] The invention relates to the application of an acetyltransferase OsG2 gene and the encoded protein thereof, and belongs to the field of genetic engineering. Background technique [0002] The way of life of plant sessile growth determines that in the entire life cycle of growth and development, when encountering an unfavorable external environment, it cannot choose to move to avoid it like animals. External conditions that adversely affect plant growth, development and reproduction are collectively referred to as adversity. Plants are mainly affected by two major adversities: biotic and abiotic adversities. Biological stress refers to the damage caused by plants, animals and microorganisms to plants, such as weeds, insects, and diseases; abiotic stress refers to excessive or insufficient physical or chemical conditions that cause damage to plant growth, development and reproduction. Adverse effects mainly include salinity, drought, high temperature, lo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/10C12N15/54C12N15/82A01H5/10A01H6/46
CPCC12N9/1029C12N15/8273C12N15/8261Y02A40/146
Inventor 侯昕杨晓霞
Owner WUHAN UNIV
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