Method for extracting and collecting peripheral blood mononuclear cells
A technology for nuclear cells and peripheral blood, applied in the field of extraction and collection of peripheral blood mononuclear cells, can solve the problems of unfavorable application, unstable cell yield and activity rate, etc., and achieve the effect of high activity and high cell yield
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Embodiment 1
[0021] A method for extracting and collecting peripheral blood mononuclear cells, comprising the following steps:
[0022] 1) Peripheral blood collection: Collect 100ml of human peripheral blood in a blood collection bag containing sodium citrate anticoagulant. After the collection is completed, the blood collection bag is refrigerated and transported in a sealed sterile transport box at a temperature of 4-8°C;
[0023] 2) Peripheral blood pretreatment: sterilize the blood collection bag containing peripheral blood transported in refrigerated storage in step 1), then transfer the peripheral blood in the blood collection bag to a 50ml centrifuge tube, take samples for counting, and perform corresponding detection;
[0024] 3) Centrifugation: Place the centrifuge tube containing the peripheral blood described in step 2) in a centrifuge, centrifuge at 2500 rpm for 10 minutes at 4°C, adjust the centrifuge to increase speed by 2 and decrease speed by 1, and the blood in the centrifu...
Embodiment 2
[0032] A method for extracting and collecting peripheral blood mononuclear cells, comprising the following steps:
[0033] 1) Peripheral blood collection: Collect 50ml of human peripheral blood in a blood collection bag containing sodium citrate anticoagulant. After the collection is completed, the blood collection bag is refrigerated in a sealed sterile transport box at a temperature of 4-8°C;
[0034] 2) Peripheral blood pretreatment: sterilize the blood collection bag containing peripheral blood transported in refrigerated storage in step 1), then transfer the peripheral blood in the blood collection bag to a 50ml centrifuge tube, take samples for counting, and perform corresponding detection;
[0035] 3) Centrifugation: place the centrifuge tube containing the peripheral blood described in step 2) in a centrifuge, and centrifuge at 2100rpm for 15min at 4°C. Respectively, the upper layer of plasma and the lower layer of blood cells;
[0036] 4) Plasma extraction: Aspirate ...
Embodiment 3
[0043] A method for extracting and collecting peripheral blood mononuclear cells, comprising the following steps:
[0044] 1) Peripheral blood collection: Collect 80ml of human peripheral blood in a blood collection bag containing sodium citrate anticoagulant. After the collection is completed, the blood collection bag is refrigerated and transported in a sealed sterile transport box, and the refrigerated temperature is 4-8 °C;
[0045] 2) Peripheral blood pretreatment: Sterilize the blood collection bag containing peripheral blood transported by refrigeration in step 1), then transfer the peripheral blood in the blood collection bag to a 50ml centrifuge tube, take samples for counting, and do corresponding detection;
[0046] 3) Centrifugation: the centrifuge tube containing peripheral blood described in step 2) was placed in a centrifuge, centrifuged at 2500rpm for 10min at 4°C, the centrifuge was adjusted to increase speed 2, decrease speed 1, and the blood in the centrifuge...
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