Method for detecting TBHQ by surface enhanced Raman spectroscopy

A surface-enhanced Raman and spectral detection technology, applied in the field of chemical analysis, can solve problems such as poor characteristics and detection interference, and achieve the effects of strong selectivity, stable reaction system, and high sensitivity

Pending Publication Date: 2021-03-09
YUNNAN TOBACCO QUALITY SUPERVISION MONITORING STATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] For this reason, the present invention provides a method for detecting TBHQ by surface-enhanced Raman spectroscopy to solve the problems of poor characteristics and serious detection interference in the existing TBHQ common detection methods

Method used

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  • Method for detecting TBHQ by surface enhanced Raman spectroscopy
  • Method for detecting TBHQ by surface enhanced Raman spectroscopy
  • Method for detecting TBHQ by surface enhanced Raman spectroscopy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: the mensuration of TBHQ in the orange essence

[0029] Step S1, preparation of Cu-I / CDs: 0.1g CuCl 2 Dissolve 0.4g of 3-iodo-L-tyrosine in 40mL of purified water, mix and dissolve, add 100μL of ethylenediamine, sonicate for 10min, transfer to a polytetrafluoroethylene reactor, heat at 180°C for 8h, and cool naturally, First filter with a filter membrane with a pore size of 0.22 μm, and then dialyze with a dialysis bag with a molecular weight cut-off of 3000D for 24 hours to obtain water-soluble Cu-I / CDs.

[0030] Step S2, preparation of composite nanozyme: 2mL of 0.25g / mL polypropyleneimine, add 20mL ultrapure water, add 1% chloroauric acid 80μL under stirring, then add 200μL Cu-I / CDs, after stirring for 30min, The solution turned reddish brown, and a composite nanozyme composed of gold nanoparticles and Cu-I / CDs was obtained.

[0031] Step S3, TBHQ oxidation product SERS spectrum detection wave number determination: Add 50-100 μL of composite nanozyme a...

Embodiment 2

[0040] Embodiment 2: the mensuration of TBHQ in peppermint essence

[0041] Step S1, preparation of Cu-I / CDs: same as Example 1;

[0042] Step S2, preparation of composite nanozyme: same as Example 1;

[0043] Step S3, TBHQ oxidation product SERS spectrum detection wavenumber determination: same as Example 1;

[0044] Step S4, TBHQ working curve making: with embodiment 1;

[0045] Step S5. Determination of TBHQ in the peppermint essence sample: Same as in Example 1, the content of TBHQ in the peppermint sample is 30.21 mg / kg.

Embodiment 3

[0046] Embodiment 3: the mensuration of TBHQ in vanilla essence

[0047] Step S1, preparation of Cu-I / CDs: same as Example 1;

[0048] Step S2, preparation of composite nanozyme: same as Example 1;

[0049] Step S3, TBHQ oxidation product SERS spectrum detection wavenumber determination: same as Example 1;

[0050] Step S4, TBHQ working curve making: with embodiment 1;

[0051] Step S5. Determination of TBHQ in the vanilla essence sample: Same as in Example 1, the content of TBHQ in the vanilla essence sample is 9.36 mg / kg.

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Abstract

The invention discloses a method for detecting TBHQ by surface enhanced Raman spectroscopy, which relates to the technical field of chemical analysis, and comprises the following steps: carrying out surface enhanced Raman spectroscopy detection on TBHQ after carrying out catalytic oxidation by using a composite nano-enzyme, making a TBHQ working curve, and determining TBHQ in a sample according tothe working curve. According to the method, Cu-I / CDs are used as a reducing agent, polypropyleneimine is used as a protective agent, chloroauric acid is reduced, a composite nano-enzyme system composed of gold nanoparticles and CuI / CDs is obtained, due to the excellent catalytic characteristic of the composite nano-enzyme system, TBHQ is selectively oxidized into a red oxidized quinones substance, and based on the surface enhanced Raman characteristic of a TBHQ oxidation product, a novel method for detecting TBHQ by surface enhanced Raman spectroscopy with high sensitivity and strong selectivity is established.

Description

technical field [0001] The invention relates to the technical field of chemical analysis, in particular to a method for detecting TBHQ by surface-enhanced Raman spectroscopy. Background technique [0002] In the production process of flavors and fragrances, some synthetic phenolic antioxidants are often added to prevent or delay the oxidative deterioration of flavors, improve their stability and extend the storage period. Adding antioxidants can delay the oxidation speed of flavors and fragrances. Tert-butyl hydroquinone (TBHQ) is a common antioxidant, compared with traditional butyl hydroxyanisole, 2,6-dibutyl hydroxytoluene and other antioxidants, its antioxidant performance is more Strong; in addition, TBHQ is relatively stable to heat, and it does not have any peculiar smell when added to oil samples. However, TBHQ has certain toxicity to the human body. Long-term storage will decompose TBHQ, and its metabolite tert-butyl-p-benzoquinone is highly toxic. GB 2760 2014 cl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 陈丹李秋兰王春琼杨德志李苓杨亚玲张燕张轲
Owner YUNNAN TOBACCO QUALITY SUPERVISION MONITORING STATION
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