Tissue transparentizing reagent and preparation method and application thereof

Abstract

The invention provides a tissue transparentizing reagent which comprises the following components in percentage by mass: 1%-10% of a depilating agent, 5%-70% of a transparentizing agent, 2%-30% of a penetration enhancer and the balance of an auxiliary agent. The tissue transparentizing reagent can make biological tissues transparent in a short time, so that experiments such as injection, imaging and the like are facilitated. The invention further provides a preparation method and application of the tissue transparentizing reagent.

Description

technical field

[0001] The application relates to the field of biomedical technology, in particular to tissue clearing reagents and their preparation methods and applications. Background technique

[0002] Mice are commonly used experimental animals in biomedical research, and their injection is a commonly used experimental method in the experimental fields of pharmacology, toxicology, genetics, tumors, and immunology. When injecting mice, the blood vessels of the mice are not easy to identify, which affects the injection and follow-up observation, and even delays the entire experimental process. Contents of the invention

[0003] In view of this, the application provides a tissue clearing reagent and a preparation method thereof. The tissue clearing reagent can make biological tissues transparent in a short period of time, thereby facilitating experiments such as injection and imaging. The field has broad application prospects.

[0004] In the first aspect, the applicat...

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