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Tissue transparentizing reagent and preparation method and application thereof

A clearing agent and tissue technology, applied in the field of biomedicine, can solve the problems of difficult identification of blood vessels in mice, affecting injection, delaying the experimental process, etc., to achieve the effect of clearing effect, simple preparation method and convenient operation.

Pending Publication Date: 2021-03-16
SHENZHEN INST OF ADVANCED TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When injecting mice, the blood vessels of the mice are not easy to identify, which affects the injection and follow-up observation, and even delays the entire experimental process.

Method used

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  • Tissue transparentizing reagent and preparation method and application thereof
  • Tissue transparentizing reagent and preparation method and application thereof
  • Tissue transparentizing reagent and preparation method and application thereof

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preparation example Construction

[0058] The present application also provides a preparation method for the above-mentioned tissue clearing agent, which includes: uniformly mixing the depilatory agent, the clearing agent, the penetration enhancer and the auxiliary agent to obtain the tissue clearing agent, wherein the mass proportion of the depilatory agent is 1%- 10%, the mass proportion of the transparent agent is 5%-70%, and the mass proportion of the penetration enhancer is 2%-30%. The preparation method of the tissue clearing reagent provided in the present application is simple and convenient to operate, which is conducive to its wide application.

[0059] The present application also provides the application of the above-mentioned tissue clearing reagent in clearing treatment of biological tissue and / or tissue imaging. In this application, the tissue clearing reagent can make biological tissue transparent in a short time, so as to meet the requirements of tissue clearing treatment, tissue imaging, etc.,...

Embodiment 1

[0063] Select sodium sulfide as depilatory agent, select glycerin as transparent agent, select thilone as penetrating agent, auxiliary agent includes water and vaseline, the mass ratio of water and vaseline is 2:1, weigh the above-mentioned content according to the mass percentage shown in Table 1 Various substances are uniformly mixed to obtain multiple groups of tissue clearing reagents.

[0064] Table 1 Example tissue clearing reagent formula table

[0065] Numbering Depilatory (%) Clarifying agent (%) Penetration enhancer (%) Auxiliary (%) 1 1 70 25 4 2 3 70 2 25 3 5 5 30 60 4 7 65 28 - 5 5 60 15 20 6 6 40 19 35 7 9 50 21 18 8 8 25 25 42 9 10 60 10 20

Embodiment 2

[0067] Calcium thioglycolate is selected as the depilatory agent, mannose and butylene glycol are selected as the transparent agent, the mass ratio of mannose and butylene glycol is 1:1, azone is selected as the penetration enhancer, and water and dodecylbenzenesulfonic acid are selected as auxiliary agents The mass ratio of sodium, water and sodium dodecylbenzenesulfonate is 1:1, and the above-mentioned substances are weighed according to the mass percentages shown in Table 1, and multiple groups of tissue clearing reagents are obtained after uniform mixing.

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Abstract

The invention provides a tissue transparentizing reagent which comprises the following components in percentage by mass: 1%-10% of a depilating agent, 5%-70% of a transparentizing agent, 2%-30% of a penetration enhancer and the balance of an auxiliary agent. The tissue transparentizing reagent can make biological tissues transparent in a short time, so that experiments such as injection, imaging and the like are facilitated. The invention further provides a preparation method and application of the tissue transparentizing reagent.

Description

technical field [0001] The application relates to the field of biomedical technology, in particular to tissue clearing reagents and their preparation methods and applications. Background technique [0002] Mice are commonly used experimental animals in biomedical research, and their injection is a commonly used experimental method in the experimental fields of pharmacology, toxicology, genetics, tumors, and immunology. When injecting mice, the blood vessels of the mice are not easy to identify, which affects the injection and follow-up observation, and even delays the entire experimental process. Contents of the invention [0003] In view of this, the application provides a tissue clearing reagent and a preparation method thereof. The tissue clearing reagent can make biological tissues transparent in a short period of time, thereby facilitating experiments such as injection and imaging. The field has broad application prospects. [0004] In the first aspect, the applicat...

Claims

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Application Information

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IPC IPC(8): G01N1/30
CPCG01N1/30G01N2001/305
Inventor 郑海荣胡德红盛宗海刘新
Owner SHENZHEN INST OF ADVANCED TECH
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