Black radish germplasm resource breeding and utilizing method

A technology of black radish and resources, applied in the field of breeding, can solve problems such as difficulty in utilization, and achieve the effects of improving utilization efficiency, protecting intellectual property rights, and reducing the risk of loss

Active Publication Date: 2021-03-23
RICE & SORGHUM INST SICHUAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Radishes consumed in the Chinese market are mainly white radishes, red radishes, green radishes and other types, and the commercial traits of black radish germplasm resources are difficult to use in breeding

Method used

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  • Black radish germplasm resource breeding and utilizing method
  • Black radish germplasm resource breeding and utilizing method
  • Black radish germplasm resource breeding and utilizing method

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Experimental program
Comparison scheme
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Embodiment

[0025] A method for breeding black radish germplasm resources, comprising the following contents.

[0026] (1) Test materials

[0027] Three black radish germplasm resources introduced from Europe ( figure 1 ) and 8 different types of radishes were used as test materials to carry out relevant research. The details of the materials are shown in Table 1.

[0028] Table 1 Details of test materials

[0029]

[0030]

[0031] (2) Material genomic DNA extraction and PCR amplification

[0032] The young leaves of radish were selected, and the DNA was extracted using the Plant DNA General Kit of Tiangen Biology (Beijing) Co., Ltd. (refer to the kit instructions for the steps).

[0033] Primers were designed according to the specific gene orf463 sequence in the radish NWB CMS mitochondrial genome (Genebank ID: MN056360) published on the NCBI website. The forward primer orf463-F sequence is as follows:

[0034] GTGCCATTGCTGGAGTGAT (5'→3') (SEQ ID NO.1);

[0035] The reverse p...

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Abstract

The invention discloses a black radish germplasm resource breeding and utilizing method which is easier to breed sterile lines compared with radish Ogura CMS, an NWB CMS sterile source created by black radishes has a wider maintainer line breeding range, almost all radish materials (including Ogura CMS hybrid varieties) without orf463 specific genes can breed corresponding maintainer lines and sterile lines, and a male sterile hybrid variety can be easily prepared from the NWB CMS sterile lines, so that the intellectual property of breeders can be better protected. Meanwhile, various restorerlines created by NWB CMS restorer genes contained in the black radishes can be used for breeding hybrid varieties and can also be used for fixing and utilizing excellent characters of NWB CMS hybrid sterile varieties on the market, the germplasm resource loss risk is reduced, and the germplasm resource utilization efficiency is improved.

Description

technical field [0001] The invention belongs to the technical field of breeding, and in particular relates to a method for breeding and utilizing black radish germplasm resources. Background technique [0002] my country is the largest producer of radish in the world, with an average annual planting area of ​​about 1.2 million hectares. Radish is a typical cross-pollination plant with serious self-depression and obvious heterosis. Using cytoplasmic male sterile line (cytoplasmic malesterility, CMS) as the female parent for hybrid seed production is currently the main way to utilize heterosis in radish. Cytoplasmic male sterility is a kind of maternal inheritance in which cytoplasmic genes and nuclear genes act together. Intra- or inter-molecular recombination or rearrangement of the mitochondrial genome can form chimeric genes or new open reading frames (ORFs) and cause infertility. This sterility can be restored by the nucleus restorer gene (Restorer fertility, Rf). [0...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/00C12Q1/6895
CPCA01H1/02A01H1/00C12Q1/6895C12Q2600/13
Inventor 李晓梅冉茂林杨峰雍晓平冉科陈琳
Owner RICE & SORGHUM INST SICHUAN ACAD OF AGRI SCI
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