Black radish germplasm resource breeding and utilizing method

Abstract

The invention discloses a black radish germplasm resource breeding and utilizing method which is easier to breed sterile lines compared with radish Ogura CMS, an NWB CMS sterile source created by black radishes has a wider maintainer line breeding range, almost all radish materials (including Ogura CMS hybrid varieties) without orf463 specific genes can breed corresponding maintainer lines and sterile lines, and a male sterile hybrid variety can be easily prepared from the NWB CMS sterile lines, so that the intellectual property of breeders can be better protected. Meanwhile, various restorerlines created by NWB CMS restorer genes contained in the black radishes can be used for breeding hybrid varieties and can also be used for fixing and utilizing excellent characters of NWB CMS hybrid sterile varieties on the market, the germplasm resource loss risk is reduced, and the germplasm resource utilization efficiency is improved.

Description

technical field

[0001] The invention belongs to the technical field of breeding, and in particular relates to a method for breeding and utilizing black radish germplasm resources. Background technique

[0002] my country is the largest producer of radish in the world, with an average annual planting area of ​​about 1.2 million hectares. Radish is a typical cross-pollination plant with serious self-depression and obvious heterosis. Using cytoplasmic male sterile line (cytoplasmic malesterility, CMS) as the female parent for hybrid seed production is currently the main way to utilize heterosis in radish. Cytoplasmic male sterility is a kind of maternal inheritance in which cytoplasmic genes and nuclear genes act together. Intra- or inter-molecular recombination or rearrangement of the mitochondrial genome can form chimeric genes or new open reading frames (ORFs) and cause infertility. This sterility can be restored by the nucleus restorer gene (Restorer fertility, Rf). [0...

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