A method for accelerating the induction of apple callus and its growth based on LED light quality
A callus and callus induction technology, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of high concentration of hormones, long time consumption, etc., and achieve the goal of reducing the amount of use and shortening the induction time. Effect
Active Publication Date: 2022-03-25
云南省农业科学院园艺作物研究所
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[0006] However, there are differences in the culture conditions required to induce callus of woody plants. In terms of induction time, it takes a long time. For Malus plants, starting from explant induction, 24-25 ° C, dark culture for 14 The callus will appear in about 10 days. After being transferred to the proliferation medium, it will generally take 60-80 days to induce an appropriate amount of callus under the same conditions. It can be seen that it takes a long time and the concentration of hormones is high.
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[0021] The method provided by the present invention is to utilize new-type light source LED, under the condition of red, green light quality ratio 1:1, the method for rapidly inducing the callus tissue of Malus plant, take apple rootstock JM7 spring leaf callus induction culture as example, specifically The process is as follows:
[0022] Callus induction stage: use the young leaves from JM7 in spring, wash them with detergent, wash them with alcohol for 30 seconds in an ultra-clean workbench, wash them twice with sterilized water, and shake them with 0.1% mercury liter for 10-12 minutes Minutes, washed 3-4 times with sterile water, cut the leaves into 1-1.5 cm squares, the back of the leaves contacted with the medium, placed 5-8 pieces in each bottle, and inoculated in the callus induction medium LS+6- BA 2.0mg / ml+2,4-D 1.0mg / ml+IAA 0.5mg / ml, pH 5.7-5.8, agar 7g / L, sucrose 30g / L, placed in the tissue culture rack with patent number ZL 2019 21299069.3, The ratio of LED red li...
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The invention provides a method for accelerating the induction of apple callus and its growth based on LED light quality, comprising: using young leaves issued in spring to inoculate the callus induction medium for cultivation, and placing it under LED red light and green light 1: 1. Illumination is carried out under the lighting conditions with a ratio of 1; the explants and the generated callus are transferred to the callus proliferation medium to continue culturing, and placed under the lighting conditions of LED red light and green light ratio for light. The method provided by the invention can improve the rate of induction and cultivation of calluses of Malus plants. The total culture time is about 40-50 days, and a large number of calluses can be cultivated. Compared with conventional dark condition cultivation, the callus induction is shortened. The time is about 8‑10 days, and the proliferation and culture time is about 40‑50 days.
Description
technical field [0001] The invention relates to the technical field of tissue culture, in particular to a method for accelerating the induction and growth of apple callus based on LED light quality. Background technique [0002] Callus is the newly formed tissue on the surface of the wound after the original plant is partially wounded. It is composed of parenchyma cells, which can be cells of various tissues in any organ of the plant, and is the basic material for cultivating cell suspensions and protoplasts. Plant callus has a variety of uses, and can be used to study plant growth and differentiation mechanisms, genetic variation rules, etc., and has special significance for plant genetics and breeding. Callus culture is not only a new method for rapid plant propagation, but also an ideal way for plant improvement, germplasm preservation and breeding. The dedifferentiation of explants is very different due to the different plant species, organ sources and physiological con...
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IPC IPC(8): A01H4/00A01G7/04
CPCY02P60/14
Inventor 黄文静杨光柱刘丹丹马钧李帆郑丽萍陈瑶
Owner 云南省农业科学院园艺作物研究所