Primer group, kit and method for detecting head and neck tumor gene survival correlation

A technology for head and neck tumors and detection methods, applied in the field of primers and kits for detection of gene survival in head and neck tumors, which can solve the problems of low research significance and low frequency of non-synonymous mutations, so as to avoid Effect of Potential Toxic Effects, Good Guidance Effects

Inactive Publication Date: 2021-04-27
AMERICA DIAGNOSIS INC
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Problems solved by technology

By counting the occurrence frequency of mutated genes in samples, it is found that the frequency of non-synonymous mutated genes in samples is too low to be of great research significance. Therefore, in exploring the effect of genetic factors o

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  • Primer group, kit and method for detecting head and neck tumor gene survival correlation
  • Primer group, kit and method for detecting head and neck tumor gene survival correlation
  • Primer group, kit and method for detecting head and neck tumor gene survival correlation

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Embodiment Construction

[0027] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.

[0028] The present invention includes the following methods:

[0029] S1: Take out the kit, equilibrate to room temperature, fully dissolve each component, and centrifuge quickly for 10s;

[0030] S2: Calculate the number of reactions (n) required for the current experiment, and distribute each reaction solution into n reaction tubes according to the dispensing volume of 23 μL / well:

[0031] n = number of samples + blank control + weak positive control

[0032] S3: Extract DNA for sample addition, add the genomic DNA of the sample to be tested, the weak positive control, and the blank control to the reaction tubes that have been filled with two kinds of PCR reaction mixtures, that is, each sample to be tested is used separately. The two kinds of PCR reaction mixtures are tested, and the amount added is 2 μL / well; the DNA concentration of the ...

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Abstract

The invention discloses a primer group, a kit and a method for detecting head and neck tumor gene survival correlation. The primer group at least comprises nucleotide sequences of two groups of primers, and a plurality of genes are preferably CDKN2A. When the CDKN2A high amplification condition is detected, patients can be recommended to accept CDKN2A inhibitor medicine treatment, so that the patients suitable for CDKN2A targeted treatment can benefit; if the CDKN2A high amplification does not exist, other treatment medicine needs to be considered in time; the condition that the patients suffer from the potential toxic effect of the medicine and unnecessary economic burden is avoided; the best treatment opportunity is missed; and good guidance effects are achieved.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a primer set, a kit and a method for detecting the survival of head and neck tumor genes. Background technique [0002] According to medical statistics, the occurrence of tumors is not random, and various cancers such as breast cancer, colorectal cancer, prostate cancer, and gastric cancer have significant family aggregation. Activation of proto-oncogenes and inactivation mutations of tumor suppressor genes play a central biological role in carcinogenesis. The main risk factors for human cancer are environmental factors, and the genetic polymorphisms of genes related to this determine the individual's susceptibility to these factors. As the number of cancer patients in the family increases and the age of onset decreases, the susceptibility intensity of tumors also increases. This susceptibility intensity increases the risk of cancer in family members along with the inheritan...

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Application Information

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IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/158
Inventor 王丹玲江勇郭成贤
Owner AMERICA DIAGNOSIS INC
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