Primer group, kit and method for detecting head and neck tumor gene survival correlation
A technology for head and neck tumors and detection methods, applied in the field of primers and kits for detection of gene survival in head and neck tumors, which can solve the problems of low research significance and low frequency of non-synonymous mutations, so as to avoid Effect of Potential Toxic Effects, Good Guidance Effects
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[0027] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.
[0028] The present invention includes the following methods:
[0029] S1: Take out the kit, equilibrate to room temperature, fully dissolve each component, and centrifuge quickly for 10s;
[0030] S2: Calculate the number of reactions (n) required for the current experiment, and distribute each reaction solution into n reaction tubes according to the dispensing volume of 23 μL / well:
[0031] n = number of samples + blank control + weak positive control
[0032] S3: Extract DNA for sample addition, add the genomic DNA of the sample to be tested, the weak positive control, and the blank control to the reaction tubes that have been filled with two kinds of PCR reaction mixtures, that is, each sample to be tested is used separately. The two kinds of PCR reaction mixtures are tested, and the amount added is 2 μL / well; the DNA concentration of the ...
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