Microfluidic impedance cytometer and preparation method thereof

Abstract

The invention provides a microfluidic impedance cytometer and a preparation method thereof. The microfluidic impedance cytometer comprises an upper electrode layer, a flow channel layer and a lower electrode layer, the flow channel layer is provided with a focusing flow channel, the upper electrode layer is provided with an excitation electrode, and the lower electrode layer is provided with an induction electrode. Both the upper electrode layer and the lower electrode layer are made of ITO conductive film materials with PET as a substrate, the flow channel layer is made of AB double-sided tape materials with PET as a substrate, and the upper surface and the lower surface of the flow channel layer are bonded with the upper electrode layer and the lower electrode layer respectively to seal the focusing flow channel. An ITO conductive film is cut, an upper insulation groove is cut in an upper electrode layer, a lower insulation groove is cut in a lower electrode layer, the upper insulation groove surrounds to form an excitation electrode independent of the rest part of the upper electrode layer, and the lower insulation groove surrounds to form an induction electrode independent of the rest part of the lower electrode layer. The excitation electrode and the induction electrode are in butt joint to form a three-dimensional differential electrode, functions of cell counting, volume measurement, focusing position monitoring and the like can be achieved, the preparation process is simple, materials are easy to obtain, and cost is low.

Description

technical field [0001] The invention relates to the technical field of microfluidic cell detection, in particular to a microfluidic impedance cytometer and a preparation method thereof. Background technique [0002] Tumor cells in peripheral blood (also known as circulating tumor cells) are often used to predict the survival period of cancer patients, and can also be used to guide cancer diagnosis and prognosis assessment, providing ideas for the development of anticancer drugs. Therefore, obtaining tumor cells quickly and efficiently from peripheral blood will be of great significance for cancer diagnosis and treatment. [0003] Existing circulating tumor cell sorting and detection devices often use immunomagnetic bead labeling and fluorescent staining to capture and detect tumor cells. Among them, the captured circulating tumor cells will lose their biological activity and cannot be used for subsequent clinical diagnosis and drug resistance detection. In addition, due to...

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Problems solved by technology

[0005] The invention provides a microfluidic impedance cytometer and a preparation method thereof, which can realize functions such as cell counting, volume measurement, and focus position monitoring. The preparation process is simple, the materials are easy to obtain and the cost is low, and the tedious preparation process in the prior art is solved. , high-cost technical problems

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