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Bacillus velezensis K01 and application thereof

A technology of Bacillus and bacteria agent, applied in Bacillus Velez K01 and its application field

Active Publication Date: 2021-05-04
GENLIDUO BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, strains with broad-spectrum antibacterial activity, multiple disease prevention and control, and plant growth promotion have not been reported, especially strains that can adapt to high-salt and other adverse environments to play a role in promoting growth.

Method used

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  • Bacillus velezensis K01 and application thereof
  • Bacillus velezensis K01 and application thereof
  • Bacillus velezensis K01 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] Isolation screening and identification of embodiment 1 bacterial strain K01

[0075] 1. Isolation and screening of strain K01

[0076] Soil was collected from Xingtai City, Hebei Province in 2012, and 10g of soil sample was added to a triangular flask containing 100ml of sterile normal saline. After standing still for 20min, the shaker was shaken at 28°C and 200rpm for 30min, and 1ml was added to 9ml of sterile normal saline. Then dilute 10 2 , 10 3 , 10 4 Take 100 μl of the above soil suspension and spread it evenly on the plate of LB medium (yeast powder 5g / L, peptone 10g / L, NaCl 5g / L, agar 15g / L) and culture it in an incubator at 28°C for 48h. Bacterial single colonies with different shapes were picked up with a sterile toothpick, streaked and purified, and stored for later use. A total of 892 strains of bacteria were isolated.

[0077] The confrontation culture method was used to detect the biocontrol effect of the isolated strains. First, Botrytis cinerea was ...

Embodiment 2

[0110] Example 2 Determination of Salt Tolerance of Bacterial Strain K01

[0111] First, the bacterial strain K01 was activated on the LB liquid medium, and the activated bacterial strain was inoculated into LB containing 5%, 9%, 13%, 15%, and 17% NaCl (w / v) according to the inoculation amount of 1%. In the liquid medium, shake culture at 28℃, 150rpm for 48h, measure its OD 600 value. At the same time, no LB medium was used as the control (CK), and each treatment was repeated 3 times. The results show that the growth of bacterial strain K01 is not significantly inhibited in the medium of salt concentration below 13%, and it is significantly inhibited in 15% NaCl solution ( Figure 6 ), indicating that strain K01 can grow well in the environment with a salt content of 13% ( Figure 7 ).

Embodiment 3

[0112] Example 3 Detection of Phosphorus Solubilizing Function of Bacterial Strain K01

[0113] First, strain K01 was activated on LB medium. Inoculate the activated bacterial strain into the phosphate-dissolving medium shown in Appendix A of NY / T1847-2010 (glucose 10.00g, (NH 4 ) 2 SO 4 0.50g, MnSO 4 ·7H 2 O 0.3g, NaCl 0.3g, KCl 0.30g, FeSO 4 4H 2 O 0.036g, MnSO 4 .4H 2 O 0.03g, distilled water 1000mL, pH7.0; calcium phytate is selected as the phosphorus source, and the addition amount is 2g; solid medium: add 1.5% agar powder in proportion). Check whether it has the function of dissolving phosphorus. After testing, K01 has the ability to dissolve phosphorus, and the diameter (D) of its dissolution circle reaches 16.07±0.48mm ( Figure 8 ).

[0114] After confirming that the strain has the phosphorus-dissolving function, the phosphorus-dissolving ability of the strain is quantitatively measured, and the available phosphorus content in the fermentation supernatant i...

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Abstract

The invention provides Bacillus velezensis K01 and application thereof. The Bacillus velezensis K01 has the growth promotion functions of dissolving phosphorus and generating indoleacetic acid, cellulase, protease and the like, also can generate anti-bacteria active substances and volatile anti-bacteria substances, can inhibit growth of various pathogenic bacteria, has a good biocontrol function, also can favorably realize colonization on the rhizosphere of a plant and in the plant, can be applied to plant growth promotion and biological control of plant diseases, and has a good application prospect.

Description

technical field [0001] The invention relates to the technical fields of microbiology, biotechnology and biological control, in particular to a strain of Bacillus Velez K01 and its application. Background technique [0002] Bacillus is a kind of aerobic or facultative anaerobic, Gram-positive bacteria that can produce spores. The bacteria are straight rods, arranged in pairs or chains. Compared with other microorganisms, the most prominent feature of Bacillus is that it can produce spores with strong stress resistance in harsh environments to survive adverse environmental conditions, and Bacillus is ubiquitous in nature, harmless to humans and animals, and harmful to humans and animals. Plants are non-pathogenic, and most bacilli are also capable of producing a wide variety of antimicrobial active substances. Therefore, Bacillus is widely studied and applied as a model research species, and has become one of the most in-depth research and most widely used plant growth-promot...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/22A01P1/00A01P3/00A01P21/00C05G3/00A23L3/3571C12R1/07
CPCA23L3/3571A01N63/22C05G3/00
Inventor 郭岩彬邢明振张云鸽王秀艳柴同海吕硕李伟欣
Owner GENLIDUO BIO TECH CO LTD
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