Kit for constant-temperature detection of respiratory tract infection bacterial pathogens by enzyme digestion probes

Abstract

The invention provides a kit for constant-temperature detection of respiratory tract infection bacterial pathogens by enzyme digestion probes. Primer probes of 12 pathogen targets are combined into four triple nucleic acid reaction solutions, and the probe for detecting each bacterial pathogen is a probe containing an RNA base and comprises 6 constant-temperature amplification primers. By utilizing the nucleic acid detection kit, detection of 12 bacterial pathogens with high frequency in respiratory tract infection can be achieved through four-single-tube multi-isothermal amplification, the kit is simple and convenient to operate, bedside detection can be achieved, the whole detection time is within 30 minutes, and screening of various bacterial pathogens in a sample to be detected can be quickly and accurately achieved.

Description

technical field [0001] The invention relates to the technical field of constant temperature detection of nucleic acids, in particular to a kit for constant temperature detection of bacterial pathogens of respiratory tract infection by enzyme-cutting probes. Background technique [0002] Respiratory tract infection is a common and high incidence clinically. Bacterial infection is a common cause of respiratory tract disease. There are many pathogenic bacteria that cause respiratory tract bacterial infection. Among adult respiratory tract bacterial infections, Staphylococcus aureus, Pseudomonas aeruginosa, Acinetobacter baumannii, Klebsiella pneumoniae, Haemophilus influenzae, Legionella pneumophila, Enterococcus, Streptococcus pneumoniae, Stenophagous maltophilia, Burkholderia cepacia, Escherichia coli, These 12 species of Nocardia astrocytes are the most common. Therefore, the realization of specific and rapid screening and detection of these 12 common bacterial pathogens in...

AI Technical Summary

Problems solved by technology

[0004] However, most of the current nucleic acid constant temperature amplification methods are difficult to perform single-tube multiple detection, or the sensitivity and specificity of multiple detection are difficult to achieve the effect of single multiple detection.

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