Fingerprint spectrum construction method of traditional Chinese medicine preparation for treating cervical spondylosis and application
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A technology of traditional Chinese medicine preparations and fingerprints, applied in the field of medical testing, can solve the problems of weak representation, inability to fully represent the quality control ability of preparations, and narrow coverage.
Active Publication Date: 2021-05-14
SHANDONG MINGREN FURUIDA PHARMA
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[0007] Invention patent CN111999423A discloses a method for establishing the fingerprint of neck pain particles and its application. The method uses notoginseng saponin R1, tetrahydropalmatine and oleanolic acid as mixed standards to construct the fingerprint o
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preparation example Construction
[0035] (1) Preparation of the test solution: take an appropriate amount of traditional Chinese medicine preparation for the treatment of cervical spondylosis, grind it into a fine powder, first use pure water for ultrasonic extraction, then use methanol for ultrasonic extraction, and combine the extracts to obtain the test solution;
[0036] (2) Preparation of mixed reference substance solution: Precisely weigh ginsenoside Rg1, ginsenoside Rb1, notoginsenoside R1, ferulic acid, tetrahydropalmatine, notopterygium alcohol, paeoniflorin, oleanolic acid, phenylethyl ferulic acid , isoimperatorin, and 11 kinds of reference substances of falcarindiol, add methanol to configure a mixed reference solution;
[0037] (3) Determination: Accurately measure the need testing solution that step (1) prepares and the reference substance solution that step (2) prepares respectively, inject high-performance liquid chromatography and measure, mobile phase is 0.1% phosphoric acid aqueous solution-a...
Embodiment 1
[0055] 1. Instruments and reagents:
[0056] Shimadzu (LC-2040C 3D) high performance liquid chromatograph; Sartorius 1 / 100,000 electronic analytical balance; fingerprint similarity evaluation software is "Chinese medicine chromatographic fingerprint similarity evaluation system" (National Pharmacopoeia Commission 2004A version); KQ-1000DE CNC ultrasonic instrument; Waters Symmetry Shield RP18 (4.6mm×250mm, 5μm) chromatographic column; The reference substances of oleanolic acid, phenylethyl ferulate, isoimperatorin, and falcarindiol were all purchased from China National Institutes for Food and Drug Control; phosphoric acid, methanol (chromatographically pure, Tianjin Concord); acetonitrile ( Chromatographically pure, Tianjin Concord); experimental water is ultrapure water (18MΩ); neck pain series samples (5 batches of neck pain granules, 5 batches of neck pain tablets, and 5 batches of neck pain capsules) were obtained from Shandong Mingrenfu Ruida Pharmaceutical Co., Ltd.
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Abstract
The invention relates to the technical field of medicine detection and particularly relates to a fingerprint spectrum construction method of a traditional Chinese medicine preparation for treating cervical spondylosis and an application. The method comprises the following steps of preparing a test solution, taking a proper amount of the traditional Chinese medicine preparation for treating cervical spondylosis, performing ultrasonic extraction with pure water and methanol in sequence, and combining extracting solutions to obtain the test solution; mixing a reference substance solution: precisely weighing 11 reference substances such as ginsenoside Rg1, ginsenoside Rb1 and notoginsenoside R1, and adding methanol to prepare the mixed reference substance solution; measurement, respectively and precisely measuring the test solution and the mixed reference substance solution, injecting into a high performance liquid chromatograph for measuring, adopting gradient elution, and recording a chromatogram; and processing the obtained spectrum by using fingerprint spectrum software to obtain the fingerprint spectrum of the traditional Chinese medicine preparation for treating cervical spondylosis. The method is advantaged in that the constructed fingerprint spectrum can more comprehensively reflect quality information of the traditional Chinese medicine preparation for treating cervical spondylosis, has advantages of rapidness, stability, high precision, strong reproducibility and the like, and can be used for quality control of the preparation; the method is simple and has good reproducibility.
Description
technical field [0001] The invention relates to the technical field of medical detection, in particular to a fingerprint construction method and application of traditional Chinese medicine preparations for treating cervical spondylosis. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] The current quantitative evaluation methods of traditional Chinese medicine use spectral or chromatographic methods to identify and measure one or several active ingredients, active ingredients or index ingredients, and most varieties only have general inspection items. Traditional Chinese medicine has always been the focus and difficulty of quality evaluation because of its complex component...
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