Anti-inflammatory tryptophan enema preparation as well as preparation method and application thereof
A tryptophan and preparation technology, applied in the field of medicine, can solve the problems of not being able to completely and effectively control the development of inflammation and affecting the quality of life of patients
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[0087] 1. Dextran sodium sulfate (DSS) modeling and animal grouping
[0088] C57BL / 6 female mice were modeled after two weeks of adaptive feeding (six weeks old). Control group: normal drinking water, lasted for 7 days and then killed; model group: 2.5% DSS drinking water, continued for 7 days, and the mice were replaced with DSS drinking water every 2-3 days during the water feeding stage. Trp intervention group: 2.5% DSS drinking water, continued for 7 days, the DSS drinking water of mice was replaced every 2-3 days during the water feeding stage, and Trp enema intervention was carried out on the 2nd, 4th, and 6th day of the experiment (the specific process is as follows: figure 1 shown);
[0089] DSS was purchased from Meiwei MP Biomedical Company, and was prepared with double-distilled water to make 2.5% DSS solution; use a 0.22 μm filter to remove impurities and bacteria, and prepare and use immediately;
[0090] During the experiment, observe and record the general con...
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