Joint detection kit for procalcitonin, interleukin 6 and heparin binding protein

A technology of heparin-binding protein and procalcitonin, which is applied in biological tests, measuring devices, material inspection products, etc., can solve the problem of no simultaneous detection kit, etc., and achieve fast and simple detection, less antibodies and raw materials, and high precision. Effect

Inactive Publication Date: 2021-06-11
RELIA BIOTECH JIANGSU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the prior art, there is no kit for simultaneous detection of PCT, IL-6 and HBP

Method used

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  • Joint detection kit for procalcitonin, interleukin 6 and heparin binding protein
  • Joint detection kit for procalcitonin, interleukin 6 and heparin binding protein
  • Joint detection kit for procalcitonin, interleukin 6 and heparin binding protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Procalcitonin, interleukin 6 and heparin binding protein combined detection kit

[0024] 1. Composition and preparation method of the kit

[0025] Procalcitonin, interleukin 6 and heparin-binding protein joint detection kit, including: test card, U disk with standard curve. This kit is referred to as the kit of the present invention. Among them, procalcitonin is abbreviated as PCT, interleukin-6 is abbreviated as IL-6, and heparin-binding protein is abbreviated as HBP.

[0026] (1) The structure of the test card

[0027] combined figure 1 and figure 2 , illustrating the structure of the test card.

[0028] The test card includes a card shell and a test strip inside the card shell.

[0029] The test strip includes a substrate 6 and a sample pad 7 , a CP pad 8 , an NC film 9 and an absorbent pad 10 pasted on the substrate in sequence. Adjacent ends of the sample pad, CP pad, NC membrane, and absorbent pad overlap each other.

[0030] The cartridge inclu...

Embodiment 3

[0072] Embodiment 3 The comparison of test card of the present invention and control test card

[0073] Control test cards 1-4 were prepared separately.

[0074]The NC film of the control test strip 1 is provided with T1 detection lines and quality control lines at equal intervals. The T1 detection line is coated with procalcitonin monoclonal antibody (manufacturer: GenScript, product number: 16A1), and the coating concentration is 1mg / mL. The quality control line was coated with rabbit anti-chicken IgY monoclonal antibody (purchased from Wuhan Yuangu Biotechnology Co., Ltd., product number AB-038B), and the coating concentration was 0.05mg / ml. According to the same method as in Example 1, procalcitonin antibody (manufacturer: GenScript, product number: 18D2) and chicken IgY (purchased from Wuhan Yuangu Biotechnology Co., Ltd., product number BCB005) were respectively labeled with biotin and mixed with fluorescein Streptavidin phase-coupled. According to the same method as ...

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Abstract

The invention provides a joint detection kit for procalcitonin, interleukin 6 and heparin binding protein, and belongs to the technical field of in-vitro diagnosis. The joint detection kit comprises a test strip, and the test strip comprises a substrate, and a sample pad, a CP pad, an NC membrane and an absorption pad which are sequentially pasted on the substrate. The CP pad is coated with a fluorescein labeled conjugate of a PCT antibody, an IL-6 antibody, an HBP antibody and chicken IgY; a detection line coated with a PCT monoclonal antibody, an IL-6 monoclonal antibody and an HBP monoclonal antibody and a quality control line coated with a rabbit anti-chicken IgY monoclonal antibody are respectively arranged on the NC membrane. According to the kit disclosed by the invention, the procalcitonin, the interleukin 6 and the heparin binding protein can be accurately and quantitatively detected at high sensitivity through one-time sampling.

Description

technical field [0001] The invention relates to a combined detection kit for procalcitonin, interleukin 6 and heparin binding protein, and belongs to the technical field of in vitro diagnosis. Background technique [0002] Procalcitonin (PCT) is a functional protein, an intermediate product in the synthesis of calcitonin, and a calcitonin propeptide substance without hormone activity. The content of PCT in plasma of healthy people is extremely low, lower than 0.5ng / ml in normal blood, and its level in plasma increases when severe bacteria, fungi, parasitic infections, sepsis and multiple organ failure occur. PCT reflects the active degree of systemic inflammatory response, is a specific indicator of severe bacterial inflammation and fungal infection, and is also a reliable indicator of sepsis and multiple organ failure syndrome related to inflammatory activities. [0003] Interleukin 6 (IL-6) is a cytokine, a type of interleukin, that can stimulate the proliferation, differ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/533G01N33/74G01N33/68
CPCG01N33/533G01N33/558G01N33/68G01N33/6869G01N33/74G01N2333/585
Inventor 沈诚刘辉荣窦彩云孙姗姗刘建源
Owner RELIA BIOTECH JIANGSU
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