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41results about How to "Lower CV value" patented technology

Method for preparing accurate quantitative detection immunochromatographic test strip

The invention provides a method for preparing an accurate quantitative detection immunochromatographic test strip. The preparation of a bonding pad comprises the steps of: S1: cutting a bonding pad: cutting the bonding pad into a size required for assembly into a finished product; S2: preparing a labeling complex: after labeling the antibody or antigen on a marker, storing the marker in a preservation solution; S3: preparing an infusion solution of the labeling complex, and mixing and diluting the diluent with the above-mentioned preservation solution containing the labeled complex to a soaking solution of the labeling complex with a certain concentration; S4: infiltrating the bonding pad: adding the infusion solution of the labeling complex into the bonding pad for infiltrating; S5: performing the bonding pad balancing process: laying the infiltrated bonding pad on a carrier stand in a constant temperature and humidity environment; S6: drying the bonding pad: drying the balanced bonding pad. The method of the invention can effectively improve the precision of the test strip for accurate quantitative detection, and has the advantages of simple preparation method, high production efficiency, low production cost and convenient industrial production.
Owner:DONGGUAN HEC MEDICAL INTELLIGENT DEVICE R&D CO LTD

Immunofluorescence detection light path mechanism, immunofluorescence detection instrument and calibration method of immunofluorescence detection instrument

The invention relates to an immunofluorescence detection light path mechanism. The immunofluorescence detection light path mechanism comprises a light source generation device, an optical two-phase sub-mirror, a first convergent lens component, a second convergent lens component and a fluorescence collection device, the optical two-phase sub-mirror is arranged on a transmission light path of a light source, the first convergent lens component and the second convergent lens component are respectively located the light path of the optical two-phase sub-mirror and a light path of transmission light, the fluorescence collection device is located on one side, far from the optical two-phase sub-mirror, of the second convergent lens component; and the first convergent lens component is configuredto do rectilinear motion along the direction of a light path of reflection light of the optical two-phase sub-mirror according to an electric signal applied on the first convergent lens component. Therefore, the fluorescence values of fluorescence test papers of a same to-be-detected sample detected by multiple immunofluorescence detection instruments are unified, the influence on the numerical value of the test result caused by the difference of parts in the instrument and the difference produced in the production and assembly is reduced, and the testing accuracy is improved. The invention further provides the immunofluorescence detection instrument and a calibration method of the immunofluorescence detection instrument.
Owner:GUANGZHOU WONDFO BIOTECH

Detection method for monomolecular protein

The invention belongs to the field of protein detection and discloses a detection method for monomolecular protein. The detection method for the monomolecular protein comprises the following steps: mixing to-be-detected protein and a target protein antibody to generate a to-be-detected protein and target protein antibody compound, mixing the to-be-detected protein and target protein antibody compound and a detection antibody marked by a marker, performing dissociation and collecting a to-be-detected protein and detection antibody compound; directly dispersing the to-be-detected protein and detection antibody compound into a micro-fluidic chip, or mixing the to-be-detected protein and detection antibody compound and a substrate corresponding to an enzyme marker, carrying out a reaction anddispersing reaction liquid into the micro-fluidic chip; performing fluorescent signal scanning by a laser confocal detection system and performing monomolecule counting. According to the detection method, the monomolecular protein can be detected quantitatively and rapidly; the detection method is high in detection sensitivity and suitable for protein with too low expression abundance. The detection method is convenient to operate, accurate in detection result and small in CV value deviation; furthermore, the flux is high and multiple samples can be detected at a time.
Owner:PILOT GENE TECH HANGZHOU CO LTD

High-speed carding machine

The invention belongs to the technical field of non-woven machines and relates to a high-speed carding machine. The high-speed carding machine comprises a main carding roll, an upper cotton discharge roll and a lower cotton discharge roll, a pair of main carding roll wallboards is fixed on a rack and at the positions respectively corresponding to the front end face and the rear end face of the main carding roll, a space between the main carding roll and the upper cotton discharge roll forms an upper triangle, and a space between the main carding roll and the lower cotton discharge roll forms a lower triangle. The high-speed carding machine is characterized in that an upper triangle airflow dissolving mechanism is arranged at the position corresponding to the upper triangle and used for dissolving airflow of the upper triangle and enabling negative pressure to be formed in the upper triangle; a lower triangle airflow dissolving mechanism is arranged at the position corresponding to the lower triangle and used for dissolving airflow of the lower triangle and enabling negative pressure to be formed in the lower triangle; the upper triangle airflow dissolving mechanism and the lower triangle airflow dissolving mechanism are respectively supported between the main carding roll wallboards. Carding rings are reduced, CV value is lowered, and lapping quality of follow-up stations is improved.
Owner:JIANGSU YINGYANG NONWOVEN MASCH CO LTD

High speed carding machine

The invention belongs to the technical field of non-woven machines and relates to a high-speed carding machine. The high-speed carding machine comprises a main carding roll, an upper cotton discharge roll and a lower cotton discharge roll, a pair of main carding roll wallboards is fixed on a rack and at the positions respectively corresponding to the front end face and the rear end face of the main carding roll, a space between the main carding roll and the upper cotton discharge roll forms an upper triangle, and a space between the main carding roll and the lower cotton discharge roll forms a lower triangle. The high-speed carding machine is characterized in that an upper triangle airflow dissolving mechanism is arranged at the position corresponding to the upper triangle and used for dissolving airflow of the upper triangle and enabling negative pressure to be formed in the upper triangle; a lower triangle airflow dissolving mechanism is arranged at the position corresponding to the lower triangle and used for dissolving airflow of the lower triangle and enabling negative pressure to be formed in the lower triangle; the upper triangle airflow dissolving mechanism and the lower triangle airflow dissolving mechanism are respectively supported between the main carding roll wallboards. Carding rings are reduced, CV value is lowered, and lapping quality of follow-up stations is improved.
Owner:JIANGSU YINGYANG NONWOVEN MASCH CO LTD

Rubber gasket lower pin

The invention discloses a rubber gasket lower pin. The shapes of any sections of the rubber gasket lower pin are the same, and the sections of the rubber gasket lower pin are basically in a T shape. The upper surface of the transversely linear part of the T shape is in a combination shape in which a straight line and a curved line are connected, the curved line part is higher than the straight line part, a height difference exists, the straight line part has a certain thickness, and the length of the straight line part is 4-4.9 millimeters. Correspondingly, the length of the curved line part which is connected with the straight line part is 20-19.1 millimeters, the distance from the highest point of the curved line part to the front edge of the straight line part is 9-9.9 millimeters, and correspondingly the distance C1 from the highest point to the tail end of the rubber gasket lower pin is 15-14.1 millimeters. The thickness of the straight line part is 2-5.3 millimeters, and correspondingly the height difference of the curved line part and the straight line part is 3.1-3.4 millimeters. The rubber gasket lower pin is simple in structure, convenient to mount and suitable for all existing ring spinning frames using curved face stair lower pins, the traction parts of the spinning frames do not need to be changed any more, and therefore the rubber gasket lower pin has wide applicability and economical efficiency.
Owner:韦莉

Water-soluble fiber and high-count cashmere yarn spinning process

The invention relates to a water-soluble fiber and high-count cashmere yarn spinning process. The process comprises the following specific steps of (1) firstly opening cashmere, adding oil and water,then preliminarily mixing the cashmere and water-soluble fibers, and feeding a mixture into a mixing willow for full mixing; (2) processing the mixture obtained in the step (1) into rough yarns by using a carding machine, and controlling the temperature of a carding workshop to be 24-26 DEG C and the humidity to be 60-65%; (3) drafting and twisting the rough yarns obtained in the step (2) by a spinning frame to form spun yarns, winding the spun yarns into yarn ears, controlling the temperature of the carding workshop to be 20-30 DEG C and the humidity to be 60-65%, and controlling the draft multiple of the spun yarns to be 1.30-1.35; and (4) performing bobbin replacement and winding on the spun yarns by using a bobbin winder, and removing relatively thin or thick slivers at the same time.According to the process, on one hand, the spinning process is improved, and on the other hand, the water-soluble fibers are modified, so that the cashmere and the water-soluble fibers can be uniformly mixed in the wool mixing stage, after the water-soluble fibers are removed through water washing, the slivers can be uniform, and the wearability of a product is good.
Owner:ZHEJIANG MAGLIA CASHMERE FAMILY CO LTD

A detection method for single molecular protein

The invention belongs to the field of protein detection, and discloses a detection method of single-molecule protein. The protein to be tested is mixed with the target protein antibody to form a complex of the protein to be tested and the target protein antibody, which is mixed with the detection antibody marked with a marker, dissociated and collected to be tested. The complex of the protein to be tested and the detection antibody; the label is a fluorescein label or an enzyme label; the complex of the protein to be tested and the detection antibody is directly dispersed on the microfluidic chip or the protein to be tested is first The complex with the detection antibody and the substrate corresponding to the enzyme label are mixed and reacted, and then the reaction solution is dispersed in the microfluidic chip; the laser confocal detection system is used for fluorescent signal scanning and single molecule counting. The detection method of the invention can quickly perform quantitative detection on single-molecule proteins, has high detection sensitivity, and is suitable for proteins whose expression abundance is too low. The detection method of the invention has the advantages of convenient operation, accurate detection result, small deviation of CV value, high throughput, and can detect multiple samples at one time.
Owner:PILOT GENE TECH HANGZHOU CO LTD

A preparation method for accurate quantitative detection of immunochromatographic test strips

ActiveCN109541234BFull infiltration diffusionEvenly distributedBiological testingAntigenEngineering
The present invention provides a method for preparing an immunochromatographic test strip for accurate quantitative detection, wherein the preparation of the binding pad includes: S1: cutting the binding pad: cutting the binding pad into the size required for assembling into a finished product; S2: Preparation of labeling complexes: after the markers are labeled with antibodies or antigens, store them in preservation solution; S3: Preparation of soaking solution for labeling complexes: dilute the diluent with the above-mentioned preservation solution containing the labeling complexes A certain concentration of the soaking solution of the labeled complex; S4: Infiltration of the binding pad: adding the soaking solution of the labeled complex to the binding pad for infiltration; S5: The process of balancing the binding pad: spreading the soaked binding pad on the carrier, Stand still in a constant temperature and humidity environment; S6: dry the bonded pad: dry the bonded pad that has been balanced. The method of the invention can effectively improve the precision of the test strip for accurate quantitative detection, and has simple preparation method, high production efficiency, low production cost, and is convenient for industrialized production.
Owner:DONGGUAN HEC MEDICAL INTELLIGENT DEVICE R&D CO LTD
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