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A preparation method for accurate quantitative detection of immunochromatographic test strips

An immunochromatography test paper and quantitative detection technology, which is applied in the field of medical testing, can solve the problems of poor test precision and inaccurate quantitative detection of immunochromatography test paper, and achieve the effects of easy reuse, low precision and easy cleaning

Active Publication Date: 2022-04-29
DONGGUAN HEC MEDICAL INTELLIGENT DEVICE R&D CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] For this reason, the technical problem to be solved by the present invention is that in the prior art, the test precision of the immunochromatographic test paper is relatively poor, and cannot be used for the problem of accurate quantitative detection, and then a kind of preparation method of the immunochromatographic test paper is provided, which can effectively Improve the precision of the test strip for accurate quantitative detection, and the preparation method is simple, the production efficiency is high, the production cost is low, and it is convenient for industrial production

Method used

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  • A preparation method for accurate quantitative detection of immunochromatographic test strips
  • A preparation method for accurate quantitative detection of immunochromatographic test strips
  • A preparation method for accurate quantitative detection of immunochromatographic test strips

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preparation example Construction

[0033] The invention provides a preparation method of an immunochromatographic test strip for accurate quantitative detection, which can effectively improve the compactness for precise quantitative detection, and has a simple preparation method, high production efficiency, low production cost, and is convenient for industrialized production. Including steps: preparation of nitrocellulose membrane, preparation of sample pad, preparation of binding pad, assembly of test strip; wherein, the preparation of the binding pad includes the following steps:

[0034] S1: Cutting the bonding pad: cutting the bonding pad to the size required for assembling the finished product;

[0035] S2: Preparation of labeling complex: after labeling the antibody or antigen with the label, store it in the preservation solution;

[0036] S3: Prepare the soaking solution of the labeled complex: mix and dilute the diluent with the above-mentioned preservation solution containing the labeled complex to obt...

Embodiment 1

[0065] Embodiment 1 Alpha-fetoprotein AFP fluorescent immunochromatography test strip and preparation method thereof

[0066] Lap the sample pad, bonding pad, NC film, and water-absorbing pad on the polyvinyl chloride PVC base plate in sequence, then cut the PVC base plate and the attached materials into test strips with a width of 3.5mm, and put them into the clamping case, and open the There are sample loading area and testing area.

[0067] Wherein, the sample pad is a glass fiber membrane CB08; the binding pad contains a complex of europium ion fluorescent microspheres labeled AFP monoclonal antibody, the material of the PVC bottom plate is polyvinyl chloride PVC plate, and the material of the NC membrane is UniSart 150 With a backing, the membrane contains: T line (test line) coated with another strain of AFP monoclonal antibody that can specifically bind to the AFP antigen to be tested, and C line (quality control line) coated with goat anti-mouse IgG antibody , the det...

Embodiment 2

[0097] Example 2 Carbohydrate antigen CA199 fluorescent immunochromatography test strip and its preparation method

[0098] Lap the sample pad, binding pad, NC film, and water-absorbing pad on the polyvinyl chloride PVC base plate in sequence, then cut the PVC base plate and the attached material into test strips with a width of 4mm, and put them into the clamping case, which has a Sample loading area and testing area.

[0099] Wherein, the sample pad is a glass fiber membrane RB45; the binding pad contains a complex of quantum dot nanospheres labeled CA199 monoclonal antibody, the PVC bottom plate is made of polyvinyl chloride PVC board, and the NC membrane material is UniSart 140 tape Backing, the membrane contains: T line (test line) coated with another strain of CA199 monoclonal antibody that can specifically bind to the CA199 antigen to be tested, C line (quality control line) coated with goat anti-mouse IgG antibody, The detection line is parallel to the quality control...

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Abstract

The present invention provides a method for preparing an immunochromatographic test strip for accurate quantitative detection, wherein the preparation of the binding pad includes: S1: cutting the binding pad: cutting the binding pad into the size required for assembling into a finished product; S2: Preparation of labeling complexes: after the markers are labeled with antibodies or antigens, store them in preservation solution; S3: Preparation of soaking solution for labeling complexes: dilute the diluent with the above-mentioned preservation solution containing the labeling complexes A certain concentration of the soaking solution of the labeled complex; S4: Infiltration of the binding pad: adding the soaking solution of the labeled complex to the binding pad for infiltration; S5: The process of balancing the binding pad: spreading the soaked binding pad on the carrier, Stand still in a constant temperature and humidity environment; S6: dry the bonded pad: dry the bonded pad that has been balanced. The method of the invention can effectively improve the precision of the test strip for accurate quantitative detection, and has simple preparation method, high production efficiency, low production cost, and is convenient for industrialized production.

Description

technical field [0001] The invention relates to the technical field of medical detection, in particular to a preparation method of an immunochromatographic test strip for accurate quantitative detection. Background technique [0002] The test strip technology is an immunological detection technology that is easy to operate, does not require professional training, is convenient and quick, and has intuitive results. At present, it is widely used in the fields of rapid diagnosis of diseases, analysis of environmental pollutants and detection of pathogenic biological factors. The detection principle is to immobilize a specific antibody (or antigen) as a capture reagent on a specific area of ​​the nitrocellulose membrane. After adding the sample to be tested, the sample to be tested moves forward along the membrane under the push of capillary force. When moving to the area where the capture reagent is immobilized, the antigen (or antibody) in the sample will specifically bind to...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/577G01N33/558G01N33/533
CPCG01N33/533G01N33/558G01N33/577G01N33/68
Inventor 郑兰花熊亮洪礼清蒋奎胜庾琼刘仁源刘勇娥李建霖
Owner DONGGUAN HEC MEDICAL INTELLIGENT DEVICE R&D CO LTD
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