Method for preparing accurate quantitative detection immunochromatographic test strip
An immunochromatographic test paper and quantitative detection technology, applied in the field of medical testing, can solve the problems of inaccurate quantitative detection, poor test precision of immunochromatographic test paper, etc., and achieve the effects of easy reuse, low imprecision, and easy cleaning.
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[0033] The invention provides a method for preparing an immunochromatographic test strip for accurate quantitative detection, which can effectively improve the compactness for accurate quantitative detection, and has a simple preparation method, high production efficiency, low production cost, and convenient industrial production. Including steps: preparation of nitrocellulose membrane, preparation of sample pad, preparation of bonding pad, assembly of test strips; wherein, the preparation of said bonding pad includes the following steps:
[0034] S1: Cutting the bonding pad: cutting the bonding pad to the size required when assembled into a finished product;
[0035] S2: Preparation of labeling complex: After labeling the antibody or antigen on the label, place it in the preservation solution;
[0036] S3: Preparation of the soaking solution of the labeled compound: mixing the diluent and the storage solution containing the labeled compound to dilute to a certain concentration of th...
Example Embodiment
[0065] Example 1 Alpha-fetoprotein AFP fluorescence immunochromatographic test strip and its preparation method
[0066] Lap the sample pad, bonding pad, NC film, and absorbent pad on the PVC bottom plate in turn, and then cut the PVC bottom plate and attached materials into test strips with a width of 3.5 mm, and put them into the card shell, and open the card shell. There are sample area and test area.
[0067] Wherein, the sample pad is a glass fiber membrane CB08; the binding pad contains a complex of europium ion fluorescent microspheres labeled AFP monoclonal antibody, the PVC bottom plate is made of polyvinyl chloride PVC board, and the NC membrane is made of UniSart 150 With a backing, the membrane contains: T line (detection line) coated with another AFP monoclonal antibody that specifically binds to the AFP antigen to be tested, and C line (quality control line) coated with goat anti-mouse IgG antibody The detection line is parallel to the quality control line with a dis...
Example Embodiment
[0097] Example 2 Carbohydrate antigen CA199 fluorescent immunochromatographic test strip and its preparation method
[0098] Lap the sample pad, bonding pad, NC film, and absorbent pad on the PVC bottom plate in turn, and then cut the PVC bottom plate and the attached materials into test strips with a width of 4 mm, and put them into the card shell. Sample area and test area.
[0099] Wherein, the sample pad is glass fiber membrane RB45; the binding pad contains a composite of quantum dot nanospheres labeled CA199 monoclonal antibody, the PVC bottom plate is made of polyvinyl chloride PVC board, and the NC membrane is made of UniSart 140 tape Backing, the membrane contains: T line (test line) coated with another CA199 monoclonal antibody that can specifically bind to the CA199 antigen to be tested, and C line (quality control line) coated with goat anti-mouse IgG antibody, The detection line is parallel to the quality control line at a distance of 4 mm, and the quantum dot nanosph...
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