Method for preparing accurate quantitative detection immunochromatographic test strip
An immunochromatographic test paper and quantitative detection technology, applied in the field of medical testing, can solve the problems of inaccurate quantitative detection, poor test precision of immunochromatographic test paper, etc., and achieve the effects of easy reuse, low imprecision, and easy cleaning.
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[0033] The invention provides a preparation method of an immunochromatographic test strip for accurate quantitative detection, which can effectively improve the compactness for precise quantitative detection, and has a simple preparation method, high production efficiency, low production cost, and is convenient for industrialized production. Including steps: preparation of nitrocellulose membrane, preparation of sample pad, preparation of binding pad, assembly of test strip; wherein, the preparation of the binding pad includes the following steps:
[0034] S1: Cutting the bonding pad: cutting the bonding pad to the size required for assembling the finished product;
[0035] S2: Preparation of labeling complex: after labeling the antibody or antigen with the label, store it in the preservation solution;
[0036] S3: Prepare the soaking solution of the labeled complex: mix and dilute the diluent with the above-mentioned preservation solution containing the labeled complex to obt...
Embodiment 1
[0065] Embodiment 1 Alpha-fetoprotein AFP fluorescent immunochromatography test strip and preparation method thereof
[0066] Lap the sample pad, bonding pad, NC film, and water-absorbing pad on the polyvinyl chloride PVC base plate in sequence, then cut the PVC base plate and the attached materials into test strips with a width of 3.5mm, and put them into the clamping case, and open the There are sample loading area and testing area.
[0067] Wherein, the sample pad is a glass fiber membrane CB08; the binding pad contains a complex of europium ion fluorescent microspheres labeled AFP monoclonal antibody, the material of the PVC bottom plate is polyvinyl chloride PVC plate, and the material of the NC membrane is UniSart 150 With a backing, the membrane contains: T line (test line) coated with another strain of AFP monoclonal antibody that can specifically bind to the AFP antigen to be tested, and C line (quality control line) coated with goat anti-mouse IgG antibody , the det...
Embodiment 2
[0097] Example 2 Carbohydrate antigen CA199 fluorescent immunochromatography test strip and its preparation method
[0098] Lap the sample pad, binding pad, NC film, and water-absorbing pad on the polyvinyl chloride PVC base plate in sequence, then cut the PVC base plate and the attached material into test strips with a width of 4mm, and put them into the clamping case, which has a Sample loading area and testing area.
[0099] Wherein, the sample pad is a glass fiber membrane RB45; the binding pad contains a complex of quantum dot nanospheres labeled CA199 monoclonal antibody, the PVC bottom plate is made of polyvinyl chloride PVC board, and the NC membrane material is UniSart 140 tape Backing, the membrane contains: T line (test line) coated with another strain of CA199 monoclonal antibody that can specifically bind to the CA199 antigen to be tested, C line (quality control line) coated with goat anti-mouse IgG antibody, The detection line is parallel to the quality control...
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