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Method for preparing accurate quantitative detection immunochromatographic test strip

An immunochromatographic test paper and quantitative detection technology, applied in the field of medical testing, can solve the problems of inaccurate quantitative detection, poor test precision of immunochromatographic test paper, etc., and achieve the effects of easy reuse, low imprecision, and easy cleaning.

Active Publication Date: 2019-03-29
DONGGUAN HEC MEDICAL INTELLIGENT DEVICE R&D CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] For this reason, the technical problem to be solved by the present invention is that in the prior art, the test precision of the immunochromatographic test paper is relatively poor, and cannot be used for the problem of accurate quantitative detection, and then a kind of preparation method of the immunochromatographic test paper is provided, which can effectively Improve the precision of the test strip for accurate quantitative detection, and the preparation method is simple, the production efficiency is high, the production cost is low, and it is convenient for industrial production

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  • Method for preparing accurate quantitative detection immunochromatographic test strip
  • Method for preparing accurate quantitative detection immunochromatographic test strip
  • Method for preparing accurate quantitative detection immunochromatographic test strip

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preparation example Construction

[0033] The invention provides a preparation method of an immunochromatographic test strip for accurate quantitative detection, which can effectively improve the compactness for precise quantitative detection, and has a simple preparation method, high production efficiency, low production cost, and is convenient for industrialized production. Including steps: preparation of nitrocellulose membrane, preparation of sample pad, preparation of binding pad, assembly of test strip; wherein, the preparation of the binding pad includes the following steps:

[0034] S1: Cutting the bonding pad: cutting the bonding pad to the size required for assembling the finished product;

[0035] S2: Preparation of labeling complex: after labeling the antibody or antigen with the label, store it in the preservation solution;

[0036] S3: Prepare the soaking solution of the labeled complex: mix and dilute the diluent with the above-mentioned preservation solution containing the labeled complex to obt...

Embodiment 1

[0065] Embodiment 1 Alpha-fetoprotein AFP fluorescent immunochromatography test strip and preparation method thereof

[0066] Lap the sample pad, bonding pad, NC film, and water-absorbing pad on the polyvinyl chloride PVC base plate in sequence, then cut the PVC base plate and the attached materials into test strips with a width of 3.5mm, and put them into the clamping case, and open the There are sample loading area and testing area.

[0067] Wherein, the sample pad is a glass fiber membrane CB08; the binding pad contains a complex of europium ion fluorescent microspheres labeled AFP monoclonal antibody, the material of the PVC bottom plate is polyvinyl chloride PVC plate, and the material of the NC membrane is UniSart 150 With a backing, the membrane contains: T line (test line) coated with another strain of AFP monoclonal antibody that can specifically bind to the AFP antigen to be tested, and C line (quality control line) coated with goat anti-mouse IgG antibody , the det...

Embodiment 2

[0097] Example 2 Carbohydrate antigen CA199 fluorescent immunochromatography test strip and its preparation method

[0098] Lap the sample pad, binding pad, NC film, and water-absorbing pad on the polyvinyl chloride PVC base plate in sequence, then cut the PVC base plate and the attached material into test strips with a width of 4mm, and put them into the clamping case, which has a Sample loading area and testing area.

[0099] Wherein, the sample pad is a glass fiber membrane RB45; the binding pad contains a complex of quantum dot nanospheres labeled CA199 monoclonal antibody, the PVC bottom plate is made of polyvinyl chloride PVC board, and the NC membrane material is UniSart 140 tape Backing, the membrane contains: T line (test line) coated with another strain of CA199 monoclonal antibody that can specifically bind to the CA199 antigen to be tested, C line (quality control line) coated with goat anti-mouse IgG antibody, The detection line is parallel to the quality control...

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Abstract

The invention provides a method for preparing an accurate quantitative detection immunochromatographic test strip. The preparation of a bonding pad comprises the steps of: S1: cutting a bonding pad: cutting the bonding pad into a size required for assembly into a finished product; S2: preparing a labeling complex: after labeling the antibody or antigen on a marker, storing the marker in a preservation solution; S3: preparing an infusion solution of the labeling complex, and mixing and diluting the diluent with the above-mentioned preservation solution containing the labeled complex to a soaking solution of the labeling complex with a certain concentration; S4: infiltrating the bonding pad: adding the infusion solution of the labeling complex into the bonding pad for infiltrating; S5: performing the bonding pad balancing process: laying the infiltrated bonding pad on a carrier stand in a constant temperature and humidity environment; S6: drying the bonding pad: drying the balanced bonding pad. The method of the invention can effectively improve the precision of the test strip for accurate quantitative detection, and has the advantages of simple preparation method, high production efficiency, low production cost and convenient industrial production.

Description

technical field [0001] The invention relates to the technical field of medical detection, in particular to a preparation method of an immunochromatographic test strip for accurate quantitative detection. Background technique [0002] The test strip technology is an immunological detection technology that is easy to operate, does not require professional training, is convenient and quick, and has intuitive results. At present, it is widely used in the fields of rapid diagnosis of diseases, analysis of environmental pollutants and detection of pathogenic biological factors. The detection principle is to immobilize a specific antibody (or antigen) as a capture reagent on a specific area of ​​the nitrocellulose membrane. After adding the sample to be tested, the sample to be tested moves forward along the membrane under the push of capillary force. When moving to the area where the capture reagent is immobilized, the antigen (or antibody) in the sample will specifically bind to...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/577G01N33/558G01N33/533
CPCG01N33/533G01N33/558G01N33/577G01N33/68
Inventor 郑兰花熊亮洪礼清蒋奎胜庾琼刘仁源刘勇娥李建霖
Owner DONGGUAN HEC MEDICAL INTELLIGENT DEVICE R&D CO LTD
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